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From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis elegans, in Comparison with ZnCl2.
Toxicol Sci. 2017 04 01; 156(2):336-343.TS

Abstract

Effects of ZnO NPs and ionic Zn on germline apoptosis and the regulation of genes in the apoptosis pathway were investigated in vivo using the model organism Caenorhabditis elegans.Age synchronized Bristol N2 worms were exposed to ZnO NPs and ZnCl2 at concentrations of 6.14 × 10-1, 61.4, and 614 μM form larval stage 1 (L1) to early adulthood. Possible ZnO nanoparticles were observed under the worm cuticle and also in the gonadal region by transmission electron microscopy (TEM). ZnO NPs and ZnCl2 both significantly increased the number of apoptotic cells as compared with controls in the 61.4 and 614 μM treatment groups (P < .05). However, ZnO NPs induced more apoptotic cells in the 61.4 μM treatment than ZnCl2 (P < .05), suggesting ZnO NP is more potent in inducing apoptosis at specific exposure concentration. Findings using the MD701 (bcIs39 [(lim-7)ced-1p::GFP + lin-15(+)]) strain further confirmed the observations in N2 strain. Genes involved in the apoptosis pathway (ced-13, ced-3, ced-4, ced-9, cep-1, dpl-1, efl-1, efl-2, egl-1, egl-38, lin-35, pax-2, and sir-2.1) were in general upregulated in response to ZnO NP exposure. The cep-1/p53 gene was up-regulated in gene expression assay. In the cep-1 loss of function mutant, no significant increase in apoptosis was observed. Therefore, the increased apoptosis resulting from ZnO NPs exposure is likely cep-1/p53 dependent. This study provides evidence that ZnO nanoparticles affect germ cell apoptotic machinery as a potential mechanism of reproductive toxicity.

Authors+Show Affiliations

Department of Biology, East Carolina University, Greenville, North Carolina. West Pharmaceutical Services, Inc. Kinston, North Carolina 28504.Junius H Rose High School, Greenville, North Carolina 27858.Department of Pharmaceutical Sciences, SUNY-Buffalo, New York, 14260.College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.Department of Pediatrics, East Carolina University, Greenville, North Carolina 27858.Department of Biology, East Carolina University, Greenville, North Carolina.Department of Biology, East Carolina University, Greenville, North Carolina.

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28003440

Citation

O'Donnell, Brittany, et al. "From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis Elegans, in Comparison With ZnCl2." Toxicological Sciences : an Official Journal of the Society of Toxicology, vol. 156, no. 2, 2017, pp. 336-343.
O'Donnell B, Huo L, Polli JR, et al. From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis elegans, in Comparison with ZnCl2. Toxicol Sci. 2017;156(2):336-343.
O'Donnell, B., Huo, L., Polli, J. R., Qiu, L., Collier, D. N., Zhang, B., & Pan, X. (2017). From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis elegans, in Comparison with ZnCl2. Toxicological Sciences : an Official Journal of the Society of Toxicology, 156(2), 336-343. https://doi.org/10.1093/toxsci/kfw258
O'Donnell B, et al. From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis Elegans, in Comparison With ZnCl2. Toxicol Sci. 2017 04 1;156(2):336-343. PubMed PMID: 28003440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - From the Cover: ZnO Nanoparticles Enhanced Germ Cell Apoptosis in Caenorhabditis elegans, in Comparison with ZnCl2. AU - O'Donnell,Brittany, AU - Huo,Lily, AU - Polli,Joseph R, AU - Qiu,Li, AU - Collier,David N, AU - Zhang,Baohong, AU - Pan,Xiaoping, PY - 2016/12/23/pubmed PY - 2018/2/16/medline PY - 2016/12/23/entrez KW - C. elegans KW - ZnO KW - apoptosis KW - cep-1/p53 pathway. KW - gene expression KW - nanoparticles SP - 336 EP - 343 JF - Toxicological sciences : an official journal of the Society of Toxicology JO - Toxicol Sci VL - 156 IS - 2 N2 - Effects of ZnO NPs and ionic Zn on germline apoptosis and the regulation of genes in the apoptosis pathway were investigated in vivo using the model organism Caenorhabditis elegans.Age synchronized Bristol N2 worms were exposed to ZnO NPs and ZnCl2 at concentrations of 6.14 × 10-1, 61.4, and 614 μM form larval stage 1 (L1) to early adulthood. Possible ZnO nanoparticles were observed under the worm cuticle and also in the gonadal region by transmission electron microscopy (TEM). ZnO NPs and ZnCl2 both significantly increased the number of apoptotic cells as compared with controls in the 61.4 and 614 μM treatment groups (P < .05). However, ZnO NPs induced more apoptotic cells in the 61.4 μM treatment than ZnCl2 (P < .05), suggesting ZnO NP is more potent in inducing apoptosis at specific exposure concentration. Findings using the MD701 (bcIs39 [(lim-7)ced-1p::GFP + lin-15(+)]) strain further confirmed the observations in N2 strain. Genes involved in the apoptosis pathway (ced-13, ced-3, ced-4, ced-9, cep-1, dpl-1, efl-1, efl-2, egl-1, egl-38, lin-35, pax-2, and sir-2.1) were in general upregulated in response to ZnO NP exposure. The cep-1/p53 gene was up-regulated in gene expression assay. In the cep-1 loss of function mutant, no significant increase in apoptosis was observed. Therefore, the increased apoptosis resulting from ZnO NPs exposure is likely cep-1/p53 dependent. This study provides evidence that ZnO nanoparticles affect germ cell apoptotic machinery as a potential mechanism of reproductive toxicity. SN - 1096-0929 UR - https://www.unboundmedicine.com/medline/citation/28003440/From_the_Cover:_ZnO_Nanoparticles_Enhanced_Germ_Cell_Apoptosis_in_Caenorhabditis_elegans_in_Comparison_with_ZnCl2_ L2 - https://academic.oup.com/toxsci/article-lookup/doi/10.1093/toxsci/kfw258 DB - PRIME DP - Unbound Medicine ER -