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Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds.
Theor Appl Genet. 2017 Mar; 130(3):609-619.TA

Abstract

KEY MESSAGE

New leaf rust adult plant resistance (APR) QTL QLr.cim - 6BL was mapped and confirmed the known pleotropic APR gene Lr46 effect on leaf rust in durum wheat line Bairds. CIMMYT-derived durum wheat line Bairds displays an adequate level of adult plant resistance (APR) to leaf rust in Mexican field environments. A recombinant inbred line (RIL) population developed from a cross of Bairds with susceptible parent Atred#1 was phenotyped for leaf rust response at Ciudad Obregon, Mexico, during 2013, 2014, 2015 and 2016 under artificially created epidemics of Puccinia triticina (Pt) race BBG/BP. The RIL population and its parents were genotyped with the 50 K diversity arrays technology (DArT) sequence system and simple sequence repeat (SSR) markers. A genetic map comprising 1150 markers was used to map the resistance loci. Four significant quantitative trait loci (QTLs) were detected on chromosomes 1BL, 2BC (centromere region), 5BL and 6BL. These QTLs, named Lr46, QLr.cim-2BC, QLr.cim-5BL and QLr.cim-6BL, respectively, explained 13.5-60.8%, 9.0-14.3%, 2.8-13.9%, and 11.6-29.4%, respectively, of leaf rust severity variation by the inclusive composite interval mapping method. All of these resistance loci were contributed by the resistant parent Bairds, except for QLr.cim-2BC, which came from susceptible parent Atred#1. Among these, the QTL on chromosome 1BL was the known pleiotropic APR gene Lr46, whereas QLr.cim-6BL, a consistently detected locus, should be a new leaf rust resistance locus in durum wheat. The mean leaf rust severity of RILs carrying all four QTLs ranged from 8.0 to 17.5%, whereas it ranged from 10.9 to 38.5% for three QTLs (Lr46 + 5BL + 6BL) derived from the resistant parent Bairds. Two RILs with four QTLs combinations can be used as sources of complex APR in durum wheat breeding.

Authors+Show Affiliations

International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Texcoco, México D.F., Mexico. c.lan@cgiar.org.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Texcoco, México D.F., Mexico.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Texcoco, México D.F., Mexico.Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, Texcoco, Edo. de México, Mexico.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Texcoco, México D.F., Mexico.Mianyang Institute of Agricultural Science/Mianyang Branch of National Wheat Improvement Center, 8 Songjiang Road, Mianyang, 621023, Sichuan, People's Republic of China.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Texcoco, México D.F., Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28004134

Citation

Lan, Caixia, et al. "Genetic Analysis and Mapping of Adult Plant Resistance Loci to Leaf Rust in Durum Wheat Cultivar Bairds." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 3, 2017, pp. 609-619.
Lan C, Basnet BR, Singh RP, et al. Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds. Theor Appl Genet. 2017;130(3):609-619.
Lan, C., Basnet, B. R., Singh, R. P., Huerta-Espino, J., Herrera-Foessel, S. A., Ren, Y., & Randhawa, M. S. (2017). Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(3), 609-619. https://doi.org/10.1007/s00122-016-2839-3
Lan C, et al. Genetic Analysis and Mapping of Adult Plant Resistance Loci to Leaf Rust in Durum Wheat Cultivar Bairds. Theor Appl Genet. 2017;130(3):609-619. PubMed PMID: 28004134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds. AU - Lan,Caixia, AU - Basnet,Bhoja R, AU - Singh,Ravi P, AU - Huerta-Espino,Julio, AU - Herrera-Foessel,Sybil A, AU - Ren,Yong, AU - Randhawa,Mandeep S, Y1 - 2016/12/21/ PY - 2016/07/01/received PY - 2016/12/01/accepted PY - 2016/12/23/pubmed PY - 2017/3/7/medline PY - 2016/12/23/entrez SP - 609 EP - 619 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 130 IS - 3 N2 - KEY MESSAGE: New leaf rust adult plant resistance (APR) QTL QLr.cim - 6BL was mapped and confirmed the known pleotropic APR gene Lr46 effect on leaf rust in durum wheat line Bairds. CIMMYT-derived durum wheat line Bairds displays an adequate level of adult plant resistance (APR) to leaf rust in Mexican field environments. A recombinant inbred line (RIL) population developed from a cross of Bairds with susceptible parent Atred#1 was phenotyped for leaf rust response at Ciudad Obregon, Mexico, during 2013, 2014, 2015 and 2016 under artificially created epidemics of Puccinia triticina (Pt) race BBG/BP. The RIL population and its parents were genotyped with the 50 K diversity arrays technology (DArT) sequence system and simple sequence repeat (SSR) markers. A genetic map comprising 1150 markers was used to map the resistance loci. Four significant quantitative trait loci (QTLs) were detected on chromosomes 1BL, 2BC (centromere region), 5BL and 6BL. These QTLs, named Lr46, QLr.cim-2BC, QLr.cim-5BL and QLr.cim-6BL, respectively, explained 13.5-60.8%, 9.0-14.3%, 2.8-13.9%, and 11.6-29.4%, respectively, of leaf rust severity variation by the inclusive composite interval mapping method. All of these resistance loci were contributed by the resistant parent Bairds, except for QLr.cim-2BC, which came from susceptible parent Atred#1. Among these, the QTL on chromosome 1BL was the known pleiotropic APR gene Lr46, whereas QLr.cim-6BL, a consistently detected locus, should be a new leaf rust resistance locus in durum wheat. The mean leaf rust severity of RILs carrying all four QTLs ranged from 8.0 to 17.5%, whereas it ranged from 10.9 to 38.5% for three QTLs (Lr46 + 5BL + 6BL) derived from the resistant parent Bairds. Two RILs with four QTLs combinations can be used as sources of complex APR in durum wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28004134/Genetic_analysis_and_mapping_of_adult_plant_resistance_loci_to_leaf_rust_in_durum_wheat_cultivar_Bairds_ L2 - https://dx.doi.org/10.1007/s00122-016-2839-3 DB - PRIME DP - Unbound Medicine ER -