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Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis.

Abstract

OBJECTIVES

To study the effect of Wenhua Juanbi Recipe (, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA).

METHODS

CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry.

RESULTS

Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups.

CONCLUSION

WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China. liuxide2001@sohu.com.

    ,

    Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.

    ,

    Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China.

    ,

    Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China.

    ,

    Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.

    Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.

    Source

    MeSH

    Animals
    Arthritis, Experimental
    Blotting, Western
    Cattle
    Drugs, Chinese Herbal
    Immunohistochemistry
    Male
    Osteoprotegerin
    RANK Ligand
    Rats, Wistar
    Receptors, Tumor Necrosis Factor, Member 14
    Synovial Membrane

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28004300

    Citation

    Liu, Xi-de, et al. "Effect of Wenhua Juanbi Recipe () On Expression of Receptor Activator of Nuclear Factor Kappa B Ligand, Osteoprotegerin, and Tumor Necrosis Factor Receptor Superfamily Member 14 in Rats With Collagen-induced Arthritis." Chinese Journal of Integrative Medicine, vol. 23, no. 3, 2017, pp. 208-214.
    Liu XD, Wang YQ, Cai L, et al. Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis. Chin J Integr Med. 2017;23(3):208-214.
    Liu, X. D., Wang, Y. Q., Cai, L., Ye, L. H., Wang, F., & Feng, Y. Y. (2017). Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis. Chinese Journal of Integrative Medicine, 23(3), pp. 208-214. doi:10.1007/s11655-016-2532-z.
    Liu XD, et al. Effect of Wenhua Juanbi Recipe () On Expression of Receptor Activator of Nuclear Factor Kappa B Ligand, Osteoprotegerin, and Tumor Necrosis Factor Receptor Superfamily Member 14 in Rats With Collagen-induced Arthritis. Chin J Integr Med. 2017;23(3):208-214. PubMed PMID: 28004300.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis. AU - Liu,Xi-de, AU - Wang,Yun-Qing, AU - Cai,Long, AU - Ye,Li-Hong, AU - Wang,Fang, AU - Feng,Ying-Ying, Y1 - 2016/12/21/ PY - 2013/07/24/received PY - 2016/12/23/pubmed PY - 2017/3/16/medline PY - 2016/12/23/entrez KW - Chinese medicine KW - Wenhua Juanbi Recipe KW - collagen-induced arthritis KW - osteoprotegerin KW - peripheral blood KW - receptor activator of nuclear factor kappa B ligand KW - synovium KW - tumor necrosis factor receptor superfamily member 14 SP - 208 EP - 214 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 23 IS - 3 N2 - OBJECTIVES: To study the effect of Wenhua Juanbi Recipe (, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA). METHODS: CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry. RESULTS: Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups. CONCLUSION: WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/28004300/Effect_of_Wenhua_Juanbi_Recipe____on_expression_of_receptor_activator_of_nuclear_factor_kappa_B_ligand_osteoprotegerin_and_tumor_necrosis_factor_receptor_superfamily_member_14_in_rats_with_collagen_induced_arthritis_ L2 - https://dx.doi.org/10.1007/s11655-016-2532-z DB - PRIME DP - Unbound Medicine ER -