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Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms.
Peptides. 2017 02; 88:80-86.P

Abstract

Three-finger toxins (3FTXs), especially α-neurotoxins, are the most poorly neutralized elapid snake toxins by current antivenoms. In this work, the conserved structural similarity and motif arrangements of long-chain α-neurotoxins led us to design peptides with consensus sequences. Eight long-chain α-neurotoxins (also known as Type II) were used to generate a consensus sequence from which two peptides were chemically synthesized, LCP1 and LCP2. Rabbit sera raised against them were able to generate partially-neutralizing antibodies, which delayed mice mortality in neutralization assays against Naja haje, Dendrospis polylepis and Ophiophagus hannah venoms.

Authors+Show Affiliations

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca Morelos 61500, Mexico.Centro de Investigación en Dinámica Celular, IICBA, UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca Morelos 61500, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca Morelos 61500, Mexico. Electronic address: corzo@ibt.unam.mx.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28010961

Citation

de la Rosa, Guillermo, et al. "Synthetic Peptide Antigens Derived From Long-chain Alpha-neurotoxins: Immunogenicity Effect Against Elapid Venoms." Peptides, vol. 88, 2017, pp. 80-86.
de la Rosa G, Pastor N, Alagón A, et al. Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms. Peptides. 2017;88:80-86.
de la Rosa, G., Pastor, N., Alagón, A., & Corzo, G. (2017). Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms. Peptides, 88, 80-86. https://doi.org/10.1016/j.peptides.2016.12.006
de la Rosa G, et al. Synthetic Peptide Antigens Derived From Long-chain Alpha-neurotoxins: Immunogenicity Effect Against Elapid Venoms. Peptides. 2017;88:80-86. PubMed PMID: 28010961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms. AU - de la Rosa,Guillermo, AU - Pastor,Nina, AU - Alagón,Alejandro, AU - Corzo,Gerardo, Y1 - 2016/12/21/ PY - 2016/10/28/received PY - 2016/12/12/revised PY - 2016/12/13/accepted PY - 2016/12/25/pubmed PY - 2017/12/1/medline PY - 2016/12/25/entrez KW - Elapid antivenom KW - Long-chain α-neurotoxins KW - Snakebites KW - Three-finger toxins SP - 80 EP - 86 JF - Peptides JO - Peptides VL - 88 N2 - Three-finger toxins (3FTXs), especially α-neurotoxins, are the most poorly neutralized elapid snake toxins by current antivenoms. In this work, the conserved structural similarity and motif arrangements of long-chain α-neurotoxins led us to design peptides with consensus sequences. Eight long-chain α-neurotoxins (also known as Type II) were used to generate a consensus sequence from which two peptides were chemically synthesized, LCP1 and LCP2. Rabbit sera raised against them were able to generate partially-neutralizing antibodies, which delayed mice mortality in neutralization assays against Naja haje, Dendrospis polylepis and Ophiophagus hannah venoms. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/28010961/Synthetic_peptide_antigens_derived_from_long_chain_alpha_neurotoxins:_Immunogenicity_effect_against_elapid_venoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(16)30242-X DB - PRIME DP - Unbound Medicine ER -