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The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice.
Mol Genet Genomics. 2017 Apr; 292(2):385-395.MG

Abstract

Ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1) plays major roles in photorespiration and primary nitrogen assimilation. However, due to no mutant or knockdown lines of OsFd-GOGAT have been reported in rice (Oryza sativa L.), the contribution of OsFd-GOGAT to rice foliar nitrogen metabolism remains little up-to-date. Here, we isolated a rice premature leaf senescence mutant named gogat1, which was reduced in 67% of the total GOGAT enzyme activity in leaves. The gogat1 mutant exhibited chlorosis under natural condition, while showed less extent premature leaf senescence under low light treatment. The gogat1 locus was mapped to a 54.1 kb region on chromosome 7, and the sequencing of OsFd-GOGAT showed one substitution (A to T) at the 3017th nucleotide of the open reading frame, leading to the amino-acid substitution of leucine changed to histidine. The gogat1 mutant showed reduced seed setting rate, while the grain protein content in gogat1 mutant was significantly higher than that in wild type. Meanwhile, during the grain-filling stage, total amino acids in the up three leaves and the upmost internode were increased dramatically. The results in this study suggested that OsFd-GOGAT might participate in nitrogen remobilization during leaf senescence, which provides a potential way to improve nitrogen use efficiency in rice.

Authors+Show Affiliations

Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou, 310058, China.Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.Department of Agronomy, Zhejiang University, Hangzhou, 310058, China. chhshi@zju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28012016

Citation

Zeng, Dong-Dong, et al. "The Ferredoxin-dependent Glutamate Synthase (OsFd-GOGAT) Participates in Leaf Senescence and the Nitrogen Remobilization in Rice." Molecular Genetics and Genomics : MGG, vol. 292, no. 2, 2017, pp. 385-395.
Zeng DD, Qin R, Li M, et al. The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice. Mol Genet Genomics. 2017;292(2):385-395.
Zeng, D. D., Qin, R., Li, M., Alamin, M., Jin, X. L., Liu, Y., & Shi, C. H. (2017). The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice. Molecular Genetics and Genomics : MGG, 292(2), 385-395. https://doi.org/10.1007/s00438-016-1275-z
Zeng DD, et al. The Ferredoxin-dependent Glutamate Synthase (OsFd-GOGAT) Participates in Leaf Senescence and the Nitrogen Remobilization in Rice. Mol Genet Genomics. 2017;292(2):385-395. PubMed PMID: 28012016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice. AU - Zeng,Dong-Dong, AU - Qin,Ran, AU - Li,Mei, AU - Alamin,Md, AU - Jin,Xiao-Li, AU - Liu,Yu, AU - Shi,Chun-Hai, Y1 - 2016/12/23/ PY - 2016/04/30/received PY - 2016/11/21/accepted PY - 2016/12/25/pubmed PY - 2017/3/30/medline PY - 2016/12/25/entrez KW - Leaf senescence KW - N remobilization KW - N use efficiency KW - OsFd-GOGAT SP - 385 EP - 395 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 292 IS - 2 N2 - Ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1) plays major roles in photorespiration and primary nitrogen assimilation. However, due to no mutant or knockdown lines of OsFd-GOGAT have been reported in rice (Oryza sativa L.), the contribution of OsFd-GOGAT to rice foliar nitrogen metabolism remains little up-to-date. Here, we isolated a rice premature leaf senescence mutant named gogat1, which was reduced in 67% of the total GOGAT enzyme activity in leaves. The gogat1 mutant exhibited chlorosis under natural condition, while showed less extent premature leaf senescence under low light treatment. The gogat1 locus was mapped to a 54.1 kb region on chromosome 7, and the sequencing of OsFd-GOGAT showed one substitution (A to T) at the 3017th nucleotide of the open reading frame, leading to the amino-acid substitution of leucine changed to histidine. The gogat1 mutant showed reduced seed setting rate, while the grain protein content in gogat1 mutant was significantly higher than that in wild type. Meanwhile, during the grain-filling stage, total amino acids in the up three leaves and the upmost internode were increased dramatically. The results in this study suggested that OsFd-GOGAT might participate in nitrogen remobilization during leaf senescence, which provides a potential way to improve nitrogen use efficiency in rice. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/28012016/The_ferredoxin_dependent_glutamate_synthase__OsFd_GOGAT__participates_in_leaf_senescence_and_the_nitrogen_remobilization_in_rice_ L2 - https://doi.org/10.1007/s00438-016-1275-z DB - PRIME DP - Unbound Medicine ER -