Tags

Type your tag names separated by a space and hit enter

Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7.
Biosens Bioelectron. 2017 May 15; 91:225-231.BB

Abstract

E. coli O157:H7 is an enterohemorrhagic bacteria responsible for serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans. Recent foodborne E. coli outbreaks has left a serious concern to public health. Therefore, there is an increasing demand for a simple, rapid and sensitive method for pathogen detection in contaminated foods. In this study, we developed a label-free electrical biosensor interfaced with graphene for sensitive detection of pathogenic bacteria. This biosensor was fabricated by interfacing graphene with interdigitated microelectrodes of capacitors that were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathogenic bacteria detection. Here, graphene nanostructures on the sensor surface provided superior chemical properties such as high carrier mobility and biocompatibility with antibodies and bacteria. The sensors transduced the signal based on changes in dielectric properties (capacitance) through (i) polarization of captured cell-surface charges, (ii) cells' internal bioactivity, (iii) cell-wall's electronegativity or dipole moment and their relaxation and (iv) charge carrier mobility of graphene that modulated the electrical properties once the pathogenic E. coli O157:H7 captured on the sensor surface. Sensitive capacitance changes thus observed with graphene based capacitors were specific to E. coli O157:H7 strain with a sensitivity as low as 10-100 cells/ml. The proposed graphene based electrical biosensor provided advantages of speed, sensitivity, specificity and in-situ bacterial detection with no chemical mediators, represents a versatile approach for detection of a wide variety of other pathogens.

Authors+Show Affiliations

Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.Faculty of Engineering and Natural Science, Sabanci University, Orhanli, Tuzla, 34956 Istanbul, Turkey.Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey. Electronic address: javed@sabanciuniv.edu.Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey. Electronic address: anjum@sabanciuniv.edu.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28012318

Citation

Pandey, Ashish, et al. "Graphene-interfaced Electrical Biosensor for Label-free and Sensitive Detection of Foodborne Pathogenic E. Coli O157:H7." Biosensors & Bioelectronics, vol. 91, 2017, pp. 225-231.
Pandey A, Gurbuz Y, Ozguz V, et al. Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7. Biosens Bioelectron. 2017;91:225-231.
Pandey, A., Gurbuz, Y., Ozguz, V., Niazi, J. H., & Qureshi, A. (2017). Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7. Biosensors & Bioelectronics, 91, 225-231. https://doi.org/10.1016/j.bios.2016.12.041
Pandey A, et al. Graphene-interfaced Electrical Biosensor for Label-free and Sensitive Detection of Foodborne Pathogenic E. Coli O157:H7. Biosens Bioelectron. 2017 May 15;91:225-231. PubMed PMID: 28012318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7. AU - Pandey,Ashish, AU - Gurbuz,Yasar, AU - Ozguz,Volkan, AU - Niazi,Javed H, AU - Qureshi,Anjum, Y1 - 2016/12/16/ PY - 2016/10/20/received PY - 2016/12/01/revised PY - 2016/12/15/accepted PY - 2016/12/25/pubmed PY - 2017/3/28/medline PY - 2016/12/25/entrez KW - Biochip KW - Biosensors KW - Dielectric KW - E. coli O157:H7 KW - Foodborne pathogen KW - Graphene SP - 225 EP - 231 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 91 N2 - E. coli O157:H7 is an enterohemorrhagic bacteria responsible for serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans. Recent foodborne E. coli outbreaks has left a serious concern to public health. Therefore, there is an increasing demand for a simple, rapid and sensitive method for pathogen detection in contaminated foods. In this study, we developed a label-free electrical biosensor interfaced with graphene for sensitive detection of pathogenic bacteria. This biosensor was fabricated by interfacing graphene with interdigitated microelectrodes of capacitors that were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathogenic bacteria detection. Here, graphene nanostructures on the sensor surface provided superior chemical properties such as high carrier mobility and biocompatibility with antibodies and bacteria. The sensors transduced the signal based on changes in dielectric properties (capacitance) through (i) polarization of captured cell-surface charges, (ii) cells' internal bioactivity, (iii) cell-wall's electronegativity or dipole moment and their relaxation and (iv) charge carrier mobility of graphene that modulated the electrical properties once the pathogenic E. coli O157:H7 captured on the sensor surface. Sensitive capacitance changes thus observed with graphene based capacitors were specific to E. coli O157:H7 strain with a sensitivity as low as 10-100 cells/ml. The proposed graphene based electrical biosensor provided advantages of speed, sensitivity, specificity and in-situ bacterial detection with no chemical mediators, represents a versatile approach for detection of a wide variety of other pathogens. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28012318/Graphene_interfaced_electrical_biosensor_for_label_free_and_sensitive_detection_of_foodborne_pathogenic_E__coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -