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Virulence factors and antimicrobial susceptibility profile of extraintestinal Escherichia coli isolated from an avian colisepticemia outbreak.
Microb Pathog. 2017 Feb; 103:119-122.MP

Abstract

In this study an avian colisepticemia outbreak was investigated. Two isolates from a chicken with colisepticemia were characterized for antimicrobial susceptibility and virulence factors profile. For this purpose 7 antimicrobial and 29 genes (fimH, hrlA/hek, iha, papC, sfa/focCD, tsh, mat, tia, gimB, ibeA, chuA, fyuA, ireA, iroN, irp2, iucD, sitD. chr., sitD. ep., iss, neuC, ompA, traT, astA, hlyA, sat, vat, pic, malX, cvi/cva) were tested. The outbreak happened in a hick chicken breeding located in the northwestern region of Rio Grande do Sul state in South of Brazil and caused 28.3% (102 deads of a total of 360 chickens) of mortality rate. Escherichia coli isolates obtained from the avian spleen and liver belong to the same phylogenetic group A and present resistance to all antimicrobials tested (ampicillin, tetracycline, gentamicin, neomycin, sulfa + trimethoprim, enrofloxacin, and norfloxacin). Both isolates harbor virulence factors related to adhesion (fimH, papC, mat), invasion (tia), iron acquisition system (iroN) and serum resistance (iss, ompA, traT), showing that these groups are important for Avian Pathogenic E. coli (APEC). However, they present different virulence profiles for some genes, whereas liver-isolate carries more hrlA/hek (adhesin), gimB (invasin), sitD ep. (iron acquisition system), sat (toxin) and hylA (toxin) genes, the spleen-isolate harbors fyuA (iron acquisition system) gene. Here, we highlight a coinfection by different strains of APEC in the same animal with colisepticemia, the great antimicrobial resistance of these bacterial isolates and the genetic traits that modulate the virulence for high mortality rate of chickens for human consumption.

Authors+Show Affiliations

Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil; Departamento de Ciências da Saúde, Curso de Farmácia, Universidade Regional Integrada do Alto Uruguai e das Missões, campus Santo Ângelo, 98802-470, Rua Universidade das Missões, 464, Santo Ângelo, Rio Grande do Sul, Brazil. Electronic address: lmatter2006@yahoo.com.br.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil. Electronic address: agueda.vargas@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28012984

Citation

Maciel, Jonas Fernandes, et al. "Virulence Factors and Antimicrobial Susceptibility Profile of Extraintestinal Escherichia Coli Isolated From an Avian Colisepticemia Outbreak." Microbial Pathogenesis, vol. 103, 2017, pp. 119-122.
Maciel JF, Matter LB, Trindade MM, et al. Virulence factors and antimicrobial susceptibility profile of extraintestinal Escherichia coli isolated from an avian colisepticemia outbreak. Microb Pathog. 2017;103:119-122.
Maciel, J. F., Matter, L. B., Trindade, M. M., Camillo, G., Lovato, M., de Ávila Botton, S., & Castagna de Vargas, A. (2017). Virulence factors and antimicrobial susceptibility profile of extraintestinal Escherichia coli isolated from an avian colisepticemia outbreak. Microbial Pathogenesis, 103, 119-122. https://doi.org/10.1016/j.micpath.2016.12.020
Maciel JF, et al. Virulence Factors and Antimicrobial Susceptibility Profile of Extraintestinal Escherichia Coli Isolated From an Avian Colisepticemia Outbreak. Microb Pathog. 2017;103:119-122. PubMed PMID: 28012984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulence factors and antimicrobial susceptibility profile of extraintestinal Escherichia coli isolated from an avian colisepticemia outbreak. AU - Maciel,Jonas Fernandes, AU - Matter,Letícia Beatriz, AU - Trindade,Michele Martins, AU - Camillo,Giovana, AU - Lovato,Maristela, AU - de Ávila Botton,Sônia, AU - Castagna de Vargas,Agueda, Y1 - 2016/12/21/ PY - 2016/11/04/received PY - 2016/12/18/revised PY - 2016/12/19/accepted PY - 2016/12/26/pubmed PY - 2017/4/7/medline PY - 2016/12/26/entrez KW - Antimicrobial susceptibility KW - Avian colisepticemia KW - Escherichia coli KW - Virulence factors SP - 119 EP - 122 JF - Microbial pathogenesis JO - Microb Pathog VL - 103 N2 - In this study an avian colisepticemia outbreak was investigated. Two isolates from a chicken with colisepticemia were characterized for antimicrobial susceptibility and virulence factors profile. For this purpose 7 antimicrobial and 29 genes (fimH, hrlA/hek, iha, papC, sfa/focCD, tsh, mat, tia, gimB, ibeA, chuA, fyuA, ireA, iroN, irp2, iucD, sitD. chr., sitD. ep., iss, neuC, ompA, traT, astA, hlyA, sat, vat, pic, malX, cvi/cva) were tested. The outbreak happened in a hick chicken breeding located in the northwestern region of Rio Grande do Sul state in South of Brazil and caused 28.3% (102 deads of a total of 360 chickens) of mortality rate. Escherichia coli isolates obtained from the avian spleen and liver belong to the same phylogenetic group A and present resistance to all antimicrobials tested (ampicillin, tetracycline, gentamicin, neomycin, sulfa + trimethoprim, enrofloxacin, and norfloxacin). Both isolates harbor virulence factors related to adhesion (fimH, papC, mat), invasion (tia), iron acquisition system (iroN) and serum resistance (iss, ompA, traT), showing that these groups are important for Avian Pathogenic E. coli (APEC). However, they present different virulence profiles for some genes, whereas liver-isolate carries more hrlA/hek (adhesin), gimB (invasin), sitD ep. (iron acquisition system), sat (toxin) and hylA (toxin) genes, the spleen-isolate harbors fyuA (iron acquisition system) gene. Here, we highlight a coinfection by different strains of APEC in the same animal with colisepticemia, the great antimicrobial resistance of these bacterial isolates and the genetic traits that modulate the virulence for high mortality rate of chickens for human consumption. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/28012984/Virulence_factors_and_antimicrobial_susceptibility_profile_of_extraintestinal_Escherichia_coli_isolated_from_an_avian_colisepticemia_outbreak_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(16)30739-2 DB - PRIME DP - Unbound Medicine ER -