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Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to OGD/R.
J Neurol Sci. 2017 Jan 15; 372:171-177.JN

Abstract

BACKGROUND

As an indispensable clinical inhalation anesthetic, sevoflurane is widely used for peri-operative sedation. The neuroprotective effect of sevoflurane pre-conditioning against cerebral ischemia/reperfusion has been gradually realized, but the underlying mechanism during the early reperfusion period has not been established.

METHOD

Primary cultured cortical neurons were treated with 2% sevoflurane pre-conditioning for 30min, exposed to oxygen-glucose deprivation for 90min, and followed by 60min of reperfusion (OGD/R). Additionally, neuronal cells were treated with an inhibitor of extracellular signal-related kinases 1 and 2 (Erk1/2) phosphorylation (PD98059), a mPTP opener (atractyloside), or a mPTP opening inhibitor (cyclosporine A) before sevoflurane pre-conditioning.

RESULT

Sevoflurane pre-conditioning decreased neuronal apoptosis (assessed by TUNEL), oxidative stress (assessed by malondialdehyde [MDA], superoxide dismutase [SOD], and heme oxygenase [HO]-1), and opening of mitochondrial permeability transition pores [mPTPs] (assessed by calcein-cobalt), but increased neuronal viability (assessed by MTT) and mitochondrial membrane potential (assessed by JC-1) after OGD/R exposure compared with OGD/R treatment alone. Pre-treatment with the mPTP opener and inhibitor of Erk1/2 phosphorylation abolished the protective effect induced by sevoflurane pre-conditioning. Pre-treatment with the mPTP opener attenuated the phosphorylation of Erk1/2 in mitochondria of neuronal cultures exposed to OGD/R induced by sevoflurane pre-conditioning. The mPTP opening inhibitor, like sevoflurane pre-conditioning, increased phosphorylation of Erk1/2 after OGD/R exposure, while PD98059 failed to reverse inhibition of mPTP opening in cultures exposed to OGD/R induced by sevoflurane pre-conditioning.

CONCLUSION

The neuroprotective mechanism of sevoflurane pre-conditioning might be associated with increased Erk1/2 phosphorylation in mitochondria via inhibition of mPTP opening in the early reperfusion period.

Authors+Show Affiliations

Department of Anesthesiology, Cangzhou Central Hospital, Cangzhou, China. Electronic address: azai2010@126.com.Department of Gerontology, Cangzhou Central Hospital, Cangzhou, China.Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China.Department of Anesthesiology, Cangzhou Central Hospital, Cangzhou, China.Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28017206

Citation

Zhang, Li-Min, et al. "Sevoflurane Pre-conditioning Increases Phosphorylation of Erk1/2 and HO-1 Expression Via Inhibition of mPTP in Primary Rat Cortical Neurons Exposed to OGD/R." Journal of the Neurological Sciences, vol. 372, 2017, pp. 171-177.
Zhang LM, Zhang DX, Zhao XC, et al. Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to OGD/R. J Neurol Sci. 2017;372:171-177.
Zhang, L. M., Zhang, D. X., Zhao, X. C., Sun, W. B., & Jiang, X. J. (2017). Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to OGD/R. Journal of the Neurological Sciences, 372, 171-177. https://doi.org/10.1016/j.jns.2016.11.055
Zhang LM, et al. Sevoflurane Pre-conditioning Increases Phosphorylation of Erk1/2 and HO-1 Expression Via Inhibition of mPTP in Primary Rat Cortical Neurons Exposed to OGD/R. J Neurol Sci. 2017 Jan 15;372:171-177. PubMed PMID: 28017206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to OGD/R. AU - Zhang,Li-Min, AU - Zhang,Dong-Xue, AU - Zhao,Xiao-Chun, AU - Sun,Wen-Bo, AU - Jiang,Xiao-Jing, Y1 - 2016/11/23/ PY - 2016/09/06/received PY - 2016/11/03/revised PY - 2016/11/22/accepted PY - 2016/12/27/entrez PY - 2016/12/27/pubmed PY - 2017/7/1/medline KW - Extracellular signal-related kinases 1/2 KW - Inhalation anesthetics KW - Mitochondria KW - Pre-conditioning SP - 171 EP - 177 JF - Journal of the neurological sciences JO - J. Neurol. Sci. VL - 372 N2 - BACKGROUND: As an indispensable clinical inhalation anesthetic, sevoflurane is widely used for peri-operative sedation. The neuroprotective effect of sevoflurane pre-conditioning against cerebral ischemia/reperfusion has been gradually realized, but the underlying mechanism during the early reperfusion period has not been established. METHOD: Primary cultured cortical neurons were treated with 2% sevoflurane pre-conditioning for 30min, exposed to oxygen-glucose deprivation for 90min, and followed by 60min of reperfusion (OGD/R). Additionally, neuronal cells were treated with an inhibitor of extracellular signal-related kinases 1 and 2 (Erk1/2) phosphorylation (PD98059), a mPTP opener (atractyloside), or a mPTP opening inhibitor (cyclosporine A) before sevoflurane pre-conditioning. RESULT: Sevoflurane pre-conditioning decreased neuronal apoptosis (assessed by TUNEL), oxidative stress (assessed by malondialdehyde [MDA], superoxide dismutase [SOD], and heme oxygenase [HO]-1), and opening of mitochondrial permeability transition pores [mPTPs] (assessed by calcein-cobalt), but increased neuronal viability (assessed by MTT) and mitochondrial membrane potential (assessed by JC-1) after OGD/R exposure compared with OGD/R treatment alone. Pre-treatment with the mPTP opener and inhibitor of Erk1/2 phosphorylation abolished the protective effect induced by sevoflurane pre-conditioning. Pre-treatment with the mPTP opener attenuated the phosphorylation of Erk1/2 in mitochondria of neuronal cultures exposed to OGD/R induced by sevoflurane pre-conditioning. The mPTP opening inhibitor, like sevoflurane pre-conditioning, increased phosphorylation of Erk1/2 after OGD/R exposure, while PD98059 failed to reverse inhibition of mPTP opening in cultures exposed to OGD/R induced by sevoflurane pre-conditioning. CONCLUSION: The neuroprotective mechanism of sevoflurane pre-conditioning might be associated with increased Erk1/2 phosphorylation in mitochondria via inhibition of mPTP opening in the early reperfusion period. SN - 1878-5883 UR - https://www.unboundmedicine.com/medline/citation/28017206/Sevoflurane_pre_conditioning_increases_phosphorylation_of_Erk1/2_and_HO_1_expression_via_inhibition_of_mPTP_in_primary_rat_cortical_neurons_exposed_to_OGD/R_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-510X(16)30758-4 DB - PRIME DP - Unbound Medicine ER -