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Major triterpenoids in Chinese hawthorn "Crataegus pinnatifida" and their effects on cell proliferation and apoptosis induction in MDA-MB-231 cancer cells.
Food Chem Toxicol. 2017 Feb; 100:149-160.FC

Abstract

The cytotoxicity and antiproliferative effect of phytochemicals presenting in the fruits of Chinese hawthorn (Crataegus pinnatifida) were evaluated. Shanlihong (Crataegus pinnatifida Bge. var. major N.E.Br.) variety possessed significant levels of flavonoids and triterpenoids, and showed potent antiproliferative effect against HepG2, MCF-7 and MDA-MB- 231 human cancer cells lines. Triterpenoids-enriched fraction (S9) prepared by Semi-preparative HPLC, and its predominant ingredient ursolic acid (UA) demonstrated remarkably antiproliferative activities for all the tested cancer cell lines. DNA flow cytometric analysis showed that S9 fraction and UA significantly induced G1 arrest in MDA-MB-231 cells in a dose-dependent manner. Western blotting analysis revealed that S9 fraction and UA significantly induced PCNA, CDK4, and Cyclin D1 downregulation in MDA-MB-231 cells, followed by p21Waf1/Cip1 up-regulation. Additionally, flow cytometer and DNA ladder assays indicated that S9 fraction and UA significantly induced MDA-MB-231 cells apoptosis. Mitochondrial death pathway was involved in this apoptosis as significantly induced caspase-9 and caspase-3 activation. These results suggested that triterpenoids-enriched fraction and UA exhibited antiproliferative activity through the cell cycle arrest and apoptosis induction, and was majorly responsible for the potent anticancer activity of Chinese hawthorn.

Authors+Show Affiliations

School of Food Science and Technology, South China University of Technology, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province, 510640, China.School of Food Science and Technology, South China University of Technology, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province, 510640, China.School of Food Science and Technology, South China University of Technology, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province, 510640, China. Electronic address: feyoulijun@scut.edu.cn.School of Food Science and Technology, South China University of Technology, Guangzhou 510640, China; Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan.Department of Food Science, Cornell University, Ithaca, NY 14853, United States.School of Food Science and Technology, South China University of Technology, Guangzhou 510640, China; Center of Guangdong Food Green Processing and Nutrition Regulation Engineering Technology, South China University of Technology, Guangzhou, Guangdong Province, 510640, China.Department of Food Science, Cornell University, Ithaca, NY 14853, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28025124

Citation

Wen, Lingrong, et al. "Major Triterpenoids in Chinese Hawthorn "Crataegus Pinnatifida" and Their Effects On Cell Proliferation and Apoptosis Induction in MDA-MB-231 Cancer Cells." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 100, 2017, pp. 149-160.
Wen L, Guo R, You L, et al. Major triterpenoids in Chinese hawthorn "Crataegus pinnatifida" and their effects on cell proliferation and apoptosis induction in MDA-MB-231 cancer cells. Food Chem Toxicol. 2017;100:149-160.
Wen, L., Guo, R., You, L., Abbasi, A. M., Li, T., Fu, X., & Liu, R. H. (2017). Major triterpenoids in Chinese hawthorn "Crataegus pinnatifida" and their effects on cell proliferation and apoptosis induction in MDA-MB-231 cancer cells. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 100, 149-160. https://doi.org/10.1016/j.fct.2016.12.032
Wen L, et al. Major Triterpenoids in Chinese Hawthorn "Crataegus Pinnatifida" and Their Effects On Cell Proliferation and Apoptosis Induction in MDA-MB-231 Cancer Cells. Food Chem Toxicol. 2017;100:149-160. PubMed PMID: 28025124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Major triterpenoids in Chinese hawthorn "Crataegus pinnatifida" and their effects on cell proliferation and apoptosis induction in MDA-MB-231 cancer cells. AU - Wen,Lingrong, AU - Guo,Ruixue, AU - You,Lijun, AU - Abbasi,Arshad Mehmood, AU - Li,Tong, AU - Fu,Xiong, AU - Liu,Rui Hai, Y1 - 2016/12/23/ PY - 2016/06/12/received PY - 2016/12/20/revised PY - 2016/12/22/accepted PY - 2016/12/28/pubmed PY - 2017/4/5/medline PY - 2016/12/28/entrez KW - Apoptosis KW - Cell cycle KW - Corosolic acid (PubChem CID: 6918774) KW - Crataegus pinnatifida KW - Epicatechin (PubChem CID: 72276) KW - MDA-MB-231 KW - Maslinic acid (PubChem CID: 73659) KW - Oleanolic acid (PubChem CID: 10494) KW - Procyanidine B(2) (PubChem CID: 122738) KW - Semi-preparative HPLC KW - Ursolic acid KW - Ursolic acid (PubChem CID: 64945) SP - 149 EP - 160 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 100 N2 - The cytotoxicity and antiproliferative effect of phytochemicals presenting in the fruits of Chinese hawthorn (Crataegus pinnatifida) were evaluated. Shanlihong (Crataegus pinnatifida Bge. var. major N.E.Br.) variety possessed significant levels of flavonoids and triterpenoids, and showed potent antiproliferative effect against HepG2, MCF-7 and MDA-MB- 231 human cancer cells lines. Triterpenoids-enriched fraction (S9) prepared by Semi-preparative HPLC, and its predominant ingredient ursolic acid (UA) demonstrated remarkably antiproliferative activities for all the tested cancer cell lines. DNA flow cytometric analysis showed that S9 fraction and UA significantly induced G1 arrest in MDA-MB-231 cells in a dose-dependent manner. Western blotting analysis revealed that S9 fraction and UA significantly induced PCNA, CDK4, and Cyclin D1 downregulation in MDA-MB-231 cells, followed by p21Waf1/Cip1 up-regulation. Additionally, flow cytometer and DNA ladder assays indicated that S9 fraction and UA significantly induced MDA-MB-231 cells apoptosis. Mitochondrial death pathway was involved in this apoptosis as significantly induced caspase-9 and caspase-3 activation. These results suggested that triterpenoids-enriched fraction and UA exhibited antiproliferative activity through the cell cycle arrest and apoptosis induction, and was majorly responsible for the potent anticancer activity of Chinese hawthorn. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/28025124/Major_triterpenoids_in_Chinese_hawthorn_"Crataegus_pinnatifida"_and_their_effects_on_cell_proliferation_and_apoptosis_induction_in_MDA_MB_231_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(16)30493-8 DB - PRIME DP - Unbound Medicine ER -