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Long non-coding RNA RPAIN regulates the invasion and apoptosis of trophoblast cell lines via complement protein C1q.
Oncotarget 2017; 8(5):7637-7646O

Abstract

Long non-coding RNAs (lncRNAs) are key regulatory molecules that are involved in a variety of biological processes and human diseases. Their impact on early onset preeclampsia remains unclear. In this study, we tested the expression of RPAIN (transcript variant 12 of RPA interacting protein, a non-coding RNA, NR_027683.1) in placenta tissues derived from 25 pregnant women with PE and 15 healthy pregnant women using quantitative real-time PCR. The effect of RPAIN on trophoblast proliferation, invasion, and apoptosis and the underlying mechanisms were examined in trophoblast cell lines (HTR-8/SVneo). The results showed that RPAIN expression levels were significantly increased in early onset preeclamptic placentas compared to normal controls. The proliferation and invasive abilities of the trophoblast cells were significantly inhibited, and the apoptosis abilities of the trophoblast cells were significantly promoted when RPAIN was overexpressed. In addition, the overexpression of RPAIN inhibited the expression of complement protein C1q. Furthermore, C1q overexpression rescued the decreased cell invasion and enhanced cell apoptosis in RPAIN-overexpressing trophoblast cells. Our results suggest that increased RPAIN levels may contribute to the development of preeclampsia through regulating trophoblast invasion and apoptosis via C1q. Therefore, we proposed RPAIN as a novel lncRNA molecule, which might contribute to the development of PE (preeclampsia) and might compose a potential diagnostic and therapeutic target for this disease.

Authors+Show Affiliations

Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China. Fourth Clinical Medicine College, Nanjing Medical University, Nanjing, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.Nanjing Maternity and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.Nanjing Maternity and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.State key Laboratory of Reproductive Medicine, Department of Plastic and Cosmetic Surgery, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.State key Laboratory of Reproductive Medicine, Department of Plastic and Cosmetic Surgery, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28032589

Citation

Song, Xuejing, et al. "Long Non-coding RNA RPAIN Regulates the Invasion and Apoptosis of Trophoblast Cell Lines Via Complement Protein C1q." Oncotarget, vol. 8, no. 5, 2017, pp. 7637-7646.
Song X, Rui C, Meng L, et al. Long non-coding RNA RPAIN regulates the invasion and apoptosis of trophoblast cell lines via complement protein C1q. Oncotarget. 2017;8(5):7637-7646.
Song, X., Rui, C., Meng, L., Zhang, R., Shen, R., Ding, H., ... Long, W. (2017). Long non-coding RNA RPAIN regulates the invasion and apoptosis of trophoblast cell lines via complement protein C1q. Oncotarget, 8(5), pp. 7637-7646. doi:10.18632/oncotarget.13826.
Song X, et al. Long Non-coding RNA RPAIN Regulates the Invasion and Apoptosis of Trophoblast Cell Lines Via Complement Protein C1q. Oncotarget. 2017 Jan 31;8(5):7637-7646. PubMed PMID: 28032589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long non-coding RNA RPAIN regulates the invasion and apoptosis of trophoblast cell lines via complement protein C1q. AU - Song,Xuejing, AU - Rui,Can, AU - Meng,Li, AU - Zhang,Rui, AU - Shen,Rong, AU - Ding,Hongjuan, AU - Li,Jun, AU - Li,Jingyun, AU - Long,Wei, PY - 2016/10/09/received PY - 2016/12/01/accepted PY - 2016/12/30/pubmed PY - 2018/3/3/medline PY - 2016/12/30/entrez KW - RPAIN KW - complement protein C1q KW - early onset preeclampsia KW - lncRNA SP - 7637 EP - 7646 JF - Oncotarget JO - Oncotarget VL - 8 IS - 5 N2 - Long non-coding RNAs (lncRNAs) are key regulatory molecules that are involved in a variety of biological processes and human diseases. Their impact on early onset preeclampsia remains unclear. In this study, we tested the expression of RPAIN (transcript variant 12 of RPA interacting protein, a non-coding RNA, NR_027683.1) in placenta tissues derived from 25 pregnant women with PE and 15 healthy pregnant women using quantitative real-time PCR. The effect of RPAIN on trophoblast proliferation, invasion, and apoptosis and the underlying mechanisms were examined in trophoblast cell lines (HTR-8/SVneo). The results showed that RPAIN expression levels were significantly increased in early onset preeclamptic placentas compared to normal controls. The proliferation and invasive abilities of the trophoblast cells were significantly inhibited, and the apoptosis abilities of the trophoblast cells were significantly promoted when RPAIN was overexpressed. In addition, the overexpression of RPAIN inhibited the expression of complement protein C1q. Furthermore, C1q overexpression rescued the decreased cell invasion and enhanced cell apoptosis in RPAIN-overexpressing trophoblast cells. Our results suggest that increased RPAIN levels may contribute to the development of preeclampsia through regulating trophoblast invasion and apoptosis via C1q. Therefore, we proposed RPAIN as a novel lncRNA molecule, which might contribute to the development of PE (preeclampsia) and might compose a potential diagnostic and therapeutic target for this disease. SN - 1949-2553 UR - https://www.unboundmedicine.com/medline/citation/28032589/Long_non_coding_RNA_RPAIN_regulates_the_invasion_and_apoptosis_of_trophoblast_cell_lines_via_complement_protein_C1q_ L2 - http://www.impactjournals.com/oncotarget/misc/linkedout.php?pii=13826 DB - PRIME DP - Unbound Medicine ER -