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[The effects of P - glycoprotein expression induced by ulinastatin on HK - 2 cells damage induced by paraquat].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016 Nov 20; 34(11):805-809.ZL

Abstract

Objective:

To investigate the protective effect of P-glycoprotein up-regulated by ulinastatin (UTI) on HK-2 cells during paraquat (PQ) -induced injury and its underlying mechanisms.

Methods:

The re- search was divided into two parts. The first part of the research was divided into normal control group, PQ group, UTI+PQ group, UTI control group. The second part of the research was divided into negative virus group (including control group, PQ group, PQU+TI group, UTI group) and P-gp siRNA group (including control group, PQ group, PQU+TI group, UTI group) . Negative virus group: the cells were transfected into the blank virus; siRNA P-gp group: the cells were transfected with P-gp siRNA virus. HK-2 cells were routinely cultured. After 800 μmol/L PQ treatment, the changes of P-gp protein levels in the HK-2 cells were determined by West-ern-blot (WB) . Then, transfected lentivirus bringing P-gp silent gene, the cell viability was determined by CCK-8 assay, the expression of P-gp in the cells after transfection was detected by WB and the concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC) .

Results:

Compared with the normal control group, the P-gp expression of PQ group had no significantly changes (P>0.05) . Compared with the PQ group, the P-gp expression of UTI+PQ group significantly increased (P>0.05) . Compared with the corre-sponding control siRNA group, the P-gp siRNA group had no significantly changes in cell viability (P>0.05) . and significantly decreased in P-gp expression. Compared with the corresponding control siRNA group, the P-gp siRNA group had no significantly changes in PQ concentration in HK-2 cell (P>0.05) , but compared with P-gp siRNA PQ group, the PQ concentration of P-gp siRNA PQ+UTI group significantly decrease (P<0.05) .

Conclusion:

UTI significantly reduced the accumulation of PQ in HK-2 cells and increased the viability of HK-2 cells in vitro may be not by increased P-gp activity. UTI could significantly reduce HK-2 cell injury induced by PQ in vitro and improve the survival rate of HK-2 cells. It may not be related to the up regulation of P-gp expres-sion.

Authors+Show Affiliations

The First College of Clinical Medical Science, China Three Gorges University, Yichang 443000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

28043264

Citation

She, X R., et al. "[The Effects of P - Glycoprotein Expression Induced By Ulinastatin On HK - 2 Cells Damage Induced By Paraquat]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 34, no. 11, 2016, pp. 805-809.
She XR, Tian X, Fan XK, et al. [The effects of P - glycoprotein expression induced by ulinastatin on HK - 2 cells damage induced by paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016;34(11):805-809.
She, X. R., Tian, X., Fan, X. K., Hong, G. L., Zhao, G. J., Li, M. F., & Lu, Z. Q. (2016). [The effects of P - glycoprotein expression induced by ulinastatin on HK - 2 cells damage induced by paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 34(11), 805-809. https://doi.org/10.3760/cma.j.issn.1001-9391.2016.11.002
She XR, et al. [The Effects of P - Glycoprotein Expression Induced By Ulinastatin On HK - 2 Cells Damage Induced By Paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016 Nov 20;34(11):805-809. PubMed PMID: 28043264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The effects of P - glycoprotein expression induced by ulinastatin on HK - 2 cells damage induced by paraquat]. AU - She,X R, AU - Tian,X, AU - Fan,X K, AU - Hong,G L, AU - Zhao,G J, AU - Li,M F, AU - Lu,Z Q, PY - 2017/1/4/entrez PY - 2017/1/4/pubmed PY - 2018/5/1/medline SP - 805 EP - 809 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 34 IS - 11 N2 - Objective: To investigate the protective effect of P-glycoprotein up-regulated by ulinastatin (UTI) on HK-2 cells during paraquat (PQ) -induced injury and its underlying mechanisms. Methods: The re- search was divided into two parts. The first part of the research was divided into normal control group, PQ group, UTI+PQ group, UTI control group. The second part of the research was divided into negative virus group (including control group, PQ group, PQU+TI group, UTI group) and P-gp siRNA group (including control group, PQ group, PQU+TI group, UTI group) . Negative virus group: the cells were transfected into the blank virus; siRNA P-gp group: the cells were transfected with P-gp siRNA virus. HK-2 cells were routinely cultured. After 800 μmol/L PQ treatment, the changes of P-gp protein levels in the HK-2 cells were determined by West-ern-blot (WB) . Then, transfected lentivirus bringing P-gp silent gene, the cell viability was determined by CCK-8 assay, the expression of P-gp in the cells after transfection was detected by WB and the concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC) . Results: Compared with the normal control group, the P-gp expression of PQ group had no significantly changes (P>0.05) . Compared with the PQ group, the P-gp expression of UTI+PQ group significantly increased (P>0.05) . Compared with the corre-sponding control siRNA group, the P-gp siRNA group had no significantly changes in cell viability (P>0.05) . and significantly decreased in P-gp expression. Compared with the corresponding control siRNA group, the P-gp siRNA group had no significantly changes in PQ concentration in HK-2 cell (P>0.05) , but compared with P-gp siRNA PQ group, the PQ concentration of P-gp siRNA PQ+UTI group significantly decrease (P<0.05) . Conclusion: UTI significantly reduced the accumulation of PQ in HK-2 cells and increased the viability of HK-2 cells in vitro may be not by increased P-gp activity. UTI could significantly reduce HK-2 cell injury induced by PQ in vitro and improve the survival rate of HK-2 cells. It may not be related to the up regulation of P-gp expres-sion. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/28043264/[The_effects_of_P___glycoprotein_expression_induced_by_ulinastatin_on_HK___2_cells_damage_induced_by_paraquat]_ L2 - https://antibodies.cancer.gov/detail/CPTC-ABCB1-4 DB - PRIME DP - Unbound Medicine ER -