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Pathological, bacteriological, and molecular characteristics of natural outbreaks of Johne's disease in goats of Fars Province, Iran.

Abstract

OBJECTIVE/BACKGROUND

Johne's disease, also called paratuberculosis, is a very important chronic infectious disease of ruminants worldwide. The causative agent is an acid-fast bacterium, Mycobacterium avium paratuberculosis (MAP). Finding a precise method for detecting MAP is essential for the control and eradication of this disease in small ruminant herds.

METHODS

In this study, appropriate samples were obtained from the ileum, cecum, colon, and mesenteric lymph nodes of 10 suspected naturally infected goats. Each sample was divided to two parts: the first part was stored at -20°C for bacteriologic culture and the second part was placed in 10% neutral formalin for molecular and histopathologic examination. To isolate MAP, the double incubation method was used for decontaminating the tissues and Middlebrook 7H9 broth-based culture associated with OADC (oleic acid, albumin, dextrose, and catalase) supplement with/without mycobactin J were used. Polymerase chain reaction (PCR; IS 900) was performed for media with positive acid-fast staining.

RESULTS

Acid-fast staining was positive in 40% of ileum samples, 50% of cecum samples, 40% of colon samples, and 50% of lymph node samples with mycobactin J and in 60% of ileum samples, 60% of cecum samples, 40% of colon samples, and 40% of lymph node samples without mycobactin J. All samples were confirmed by IS 900 PCR assay. Diffuse granulomatous enteritis with multibacillary lesions and paucibacillary multifocal lymphadenitis associated with calcification were diagnosed histopathologically.

CONCLUSION

MAP detection in intestinal content and in tissues is quite necessary for the diagnosis, control, and eradication of this disease in small ruminant herds.

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  • Authors+Show Affiliations

    ,

    Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz 71345-1731, Iran. Electronic address: forugh_zarei@yahoo.com.

    ,

    Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz 71345-1731, Iran.

    Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz 71345-1731, Iran.

    Source

    International journal of mycobacteriology 5 Suppl 1: 2016 Dec pg S202

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28043554

    Citation

    Zarei Kordshouli, F, et al. "Pathological, Bacteriological, and Molecular Characteristics of Natural Outbreaks of Johne's Disease in Goats of Fars Province, Iran." International Journal of Mycobacteriology, vol. 5 Suppl 1, 2016, pp. S202.
    Zarei Kordshouli F, Khodakaram Tafti A, Haghkhah M. Pathological, bacteriological, and molecular characteristics of natural outbreaks of Johne's disease in goats of Fars Province, Iran. Int J Mycobacteriol. 2016;5 Suppl 1:S202.
    Zarei Kordshouli, F., Khodakaram Tafti, A., & Haghkhah, M. (2016). Pathological, bacteriological, and molecular characteristics of natural outbreaks of Johne's disease in goats of Fars Province, Iran. International Journal of Mycobacteriology, 5 Suppl 1, pp. S202. doi:10.1016/j.ijmyco.2016.11.016.
    Zarei Kordshouli F, Khodakaram Tafti A, Haghkhah M. Pathological, Bacteriological, and Molecular Characteristics of Natural Outbreaks of Johne's Disease in Goats of Fars Province, Iran. Int J Mycobacteriol. 2016;5 Suppl 1:S202. PubMed PMID: 28043554.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Pathological, bacteriological, and molecular characteristics of natural outbreaks of Johne's disease in goats of Fars Province, Iran. AU - Zarei Kordshouli,F, AU - Khodakaram Tafti,A, AU - Haghkhah,M, Y1 - 2016/11/24/ PY - 2016/10/17/received PY - 2016/11/06/accepted PY - 2017/1/4/entrez PY - 2017/1/4/pubmed PY - 2017/1/4/medline KW - Culture KW - Goat KW - IS 900 KW - Paratuberculosis KW - Pathology SP - S202 EP - S202 JF - International journal of mycobacteriology JO - Int J Mycobacteriol VL - 5 Suppl 1 N2 - OBJECTIVE/BACKGROUND: Johne's disease, also called paratuberculosis, is a very important chronic infectious disease of ruminants worldwide. The causative agent is an acid-fast bacterium, Mycobacterium avium paratuberculosis (MAP). Finding a precise method for detecting MAP is essential for the control and eradication of this disease in small ruminant herds. METHODS: In this study, appropriate samples were obtained from the ileum, cecum, colon, and mesenteric lymph nodes of 10 suspected naturally infected goats. Each sample was divided to two parts: the first part was stored at -20°C for bacteriologic culture and the second part was placed in 10% neutral formalin for molecular and histopathologic examination. To isolate MAP, the double incubation method was used for decontaminating the tissues and Middlebrook 7H9 broth-based culture associated with OADC (oleic acid, albumin, dextrose, and catalase) supplement with/without mycobactin J were used. Polymerase chain reaction (PCR; IS 900) was performed for media with positive acid-fast staining. RESULTS: Acid-fast staining was positive in 40% of ileum samples, 50% of cecum samples, 40% of colon samples, and 50% of lymph node samples with mycobactin J and in 60% of ileum samples, 60% of cecum samples, 40% of colon samples, and 40% of lymph node samples without mycobactin J. All samples were confirmed by IS 900 PCR assay. Diffuse granulomatous enteritis with multibacillary lesions and paucibacillary multifocal lymphadenitis associated with calcification were diagnosed histopathologically. CONCLUSION: MAP detection in intestinal content and in tissues is quite necessary for the diagnosis, control, and eradication of this disease in small ruminant herds. SN - 2212-554X UR - https://www.unboundmedicine.com/medline/citation/28043554/Pathological,_bacteriological,_and_molecular_characteristics_of_natural_outbreaks_of_Johne's_disease_in_goats_of_Fars_Province,_Iran L2 - https://linkinghub.elsevier.com/retrieve/pii/S2212-5531(16)30238-2 DB - PRIME DP - Unbound Medicine ER -