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Transcript analysis in two alfalfa salt tolerance selected breeding populations relative to a non-tolerant population.
Genome. 2017 Feb; 60(2):104-127.G

Abstract

With the growing limitations on arable land, alfalfa (a widely cultivated, low-input forage) is now being selected to extend cultivation into saline lands for low-cost biofeedstock purposes. Here, minerals and transcriptome profiles were compared between two new salinity-tolerant North American alfalfa breeding populations and a more salinity-sensitive western Canadian alfalfa population grown under hydroponic saline conditions. All three populations accumulated two-fold higher sodium in roots than shoots as a function of increased electrical conductivity. At least 50% of differentially expressed genes (p < 0.05) were down-regulated in the salt-sensitive population growing under high salinity, while expression remained unchanged in the saline-tolerant populations. In particular, most reduction in transcript levels in the salt-sensitive population was observed in genes specifying cell wall structural components, lipids, secondary metabolism, auxin and ethylene hormones, development, transport, signalling, heat shock, proteolysis, pathogenesis-response, abiotic stress, RNA processing, and protein metabolism. Transcript diversity for transcription factors, protein modification, and protein degradation genes was also more strongly affected in salt-tolerant CW064027 than in salt-tolerant Bridgeview and salt-sensitive Rangelander, while both saline-tolerant populations showed more substantial up-regulation in redox-related genes and B-ZIP transcripts. The report highlights the first use of bulked genotypes as replicated samples to compare the transcriptomes of obligate out-cross breeding populations in alfalfa.

Authors+Show Affiliations

a Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7J 0X2, Canada. b Department of Computing Science, University of Alberta, 2-21 Athabasca Hall, Edmonton, AB T6G 2R3, Canada.b Department of Computing Science, University of Alberta, 2-21 Athabasca Hall, Edmonton, AB T6G 2R3, Canada.a Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7J 0X2, Canada.c Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, P.O. Box 1030, Swift Current, SK S9H 3X2, Canada.c Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, P.O. Box 1030, Swift Current, SK S9H 3X2, Canada.c Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, P.O. Box 1030, Swift Current, SK S9H 3X2, Canada.d National Research Council, 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada.e AAFC Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 - 1st Avenue S., Lethbridge, AB T1J 4B1, Canada.b Department of Computing Science, University of Alberta, 2-21 Athabasca Hall, Edmonton, AB T6G 2R3, Canada. f Department of Biological Sciences, University of Alberta, 11455 Saskatchewan Drive, Edmonton, AB T6G 2R3, Canada.g Alforex Seeds, an affiliate of Dow AgroSciences, N4505 CTH M, West Salem, WI 54669, USA.g Alforex Seeds, an affiliate of Dow AgroSciences, N4505 CTH M, West Salem, WI 54669, USA.a Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7J 0X2, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28045337

Citation

Gruber, M Y., et al. "Transcript Analysis in Two Alfalfa Salt Tolerance Selected Breeding Populations Relative to a Non-tolerant Population." Genome, vol. 60, no. 2, 2017, pp. 104-127.
Gruber MY, Xia J, Yu M, et al. Transcript analysis in two alfalfa salt tolerance selected breeding populations relative to a non-tolerant population. Genome. 2017;60(2):104-127.
Gruber, M. Y., Xia, J., Yu, M., Steppuhn, H., Wall, K., Messer, D., Sharpe, A. G., Acharya, S. N., Wishart, D. S., Johnson, D., Miller, D. R., & Taheri, A. (2017). Transcript analysis in two alfalfa salt tolerance selected breeding populations relative to a non-tolerant population. Genome, 60(2), 104-127. https://doi.org/10.1139/gen-2016-0111
Gruber MY, et al. Transcript Analysis in Two Alfalfa Salt Tolerance Selected Breeding Populations Relative to a Non-tolerant Population. Genome. 2017;60(2):104-127. PubMed PMID: 28045337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcript analysis in two alfalfa salt tolerance selected breeding populations relative to a non-tolerant population. AU - Gruber,M Y, AU - Xia,J, AU - Yu,M, AU - Steppuhn,H, AU - Wall,K, AU - Messer,D, AU - Sharpe,A G, AU - Acharya,S N, AU - Wishart,D S, AU - Johnson,D, AU - Miller,D R, AU - Taheri,A, Y1 - 2016/10/07/ PY - 2017/1/4/pubmed PY - 2017/2/24/medline PY - 2017/1/4/entrez KW - RNA-Seq KW - abiotic stress KW - alfalfa KW - luzerne KW - salinity KW - salinité KW - stress abiotique KW - transcriptome SP - 104 EP - 127 JF - Genome JO - Genome VL - 60 IS - 2 N2 - With the growing limitations on arable land, alfalfa (a widely cultivated, low-input forage) is now being selected to extend cultivation into saline lands for low-cost biofeedstock purposes. Here, minerals and transcriptome profiles were compared between two new salinity-tolerant North American alfalfa breeding populations and a more salinity-sensitive western Canadian alfalfa population grown under hydroponic saline conditions. All three populations accumulated two-fold higher sodium in roots than shoots as a function of increased electrical conductivity. At least 50% of differentially expressed genes (p < 0.05) were down-regulated in the salt-sensitive population growing under high salinity, while expression remained unchanged in the saline-tolerant populations. In particular, most reduction in transcript levels in the salt-sensitive population was observed in genes specifying cell wall structural components, lipids, secondary metabolism, auxin and ethylene hormones, development, transport, signalling, heat shock, proteolysis, pathogenesis-response, abiotic stress, RNA processing, and protein metabolism. Transcript diversity for transcription factors, protein modification, and protein degradation genes was also more strongly affected in salt-tolerant CW064027 than in salt-tolerant Bridgeview and salt-sensitive Rangelander, while both saline-tolerant populations showed more substantial up-regulation in redox-related genes and B-ZIP transcripts. The report highlights the first use of bulked genotypes as replicated samples to compare the transcriptomes of obligate out-cross breeding populations in alfalfa. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/28045337/Transcript_analysis_in_two_alfalfa_salt_tolerance_selected_breeding_populations_relative_to_a_non_tolerant_population_ DB - PRIME DP - Unbound Medicine ER -