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IgA isotyping of antigliadin antibodies. A possible clue for a less invasive diagnosis of coeliac disease.
Clin Chim Acta. 1989 Aug 31; 183(3):285-94.CC

Abstract

An enzyme-linked immunosorbent assay was used to measure serum antigliadin antibodies (AGA) of IgG and IgA classes. The assay was modified to measure IgA1 and IgA2 subclasses with monoclonal anti-IgA subclass antibodies. Serum IgG- and IgA-AGA levels were elevated in patients with coeliac disease (CD) but an overlap was seen with control sera. IgA-AGA isotyping using monoclonal anti-human IgA1 and IgA2 antibodies increased the sensitivity and specificity of the assay to almost 100%. All patients with active untreated CD and none of the control groups had elevated IgA1-AGA and IgA2-AGA. In order to measure the relative distribution of IgA1-AGA versus IgA2-AGA an IgA1/IgA2 ratio was calculated. In patients with active untreated CD a ratio of 2.8 was found, declining to 2.2 during treatment. A gluten challenge increased the ratio to 3.4. These findings suggest that IgA1-AGA subclass measurements are a useful screening test before small bowel biopsies are performed. This method can also be used to assess the results of a gluten free diet.

Authors+Show Affiliations

Department of Internal Medicine, State University of Ghent, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2805354

Citation

Elewaut, A, et al. "IgA Isotyping of Antigliadin Antibodies. a Possible Clue for a Less Invasive Diagnosis of Coeliac Disease." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 183, no. 3, 1989, pp. 285-94.
Elewaut A, Dacremont G, Robberecht E, et al. IgA isotyping of antigliadin antibodies. A possible clue for a less invasive diagnosis of coeliac disease. Clin Chim Acta. 1989;183(3):285-94.
Elewaut, A., Dacremont, G., Robberecht, E., Leroy, J., & De Baets, M. H. (1989). IgA isotyping of antigliadin antibodies. A possible clue for a less invasive diagnosis of coeliac disease. Clinica Chimica Acta; International Journal of Clinical Chemistry, 183(3), 285-94.
Elewaut A, et al. IgA Isotyping of Antigliadin Antibodies. a Possible Clue for a Less Invasive Diagnosis of Coeliac Disease. Clin Chim Acta. 1989 Aug 31;183(3):285-94. PubMed PMID: 2805354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgA isotyping of antigliadin antibodies. A possible clue for a less invasive diagnosis of coeliac disease. AU - Elewaut,A, AU - Dacremont,G, AU - Robberecht,E, AU - Leroy,J, AU - De Baets,M H, PY - 1989/8/31/pubmed PY - 1989/8/31/medline PY - 1989/8/31/entrez SP - 285 EP - 94 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 183 IS - 3 N2 - An enzyme-linked immunosorbent assay was used to measure serum antigliadin antibodies (AGA) of IgG and IgA classes. The assay was modified to measure IgA1 and IgA2 subclasses with monoclonal anti-IgA subclass antibodies. Serum IgG- and IgA-AGA levels were elevated in patients with coeliac disease (CD) but an overlap was seen with control sera. IgA-AGA isotyping using monoclonal anti-human IgA1 and IgA2 antibodies increased the sensitivity and specificity of the assay to almost 100%. All patients with active untreated CD and none of the control groups had elevated IgA1-AGA and IgA2-AGA. In order to measure the relative distribution of IgA1-AGA versus IgA2-AGA an IgA1/IgA2 ratio was calculated. In patients with active untreated CD a ratio of 2.8 was found, declining to 2.2 during treatment. A gluten challenge increased the ratio to 3.4. These findings suggest that IgA1-AGA subclass measurements are a useful screening test before small bowel biopsies are performed. This method can also be used to assess the results of a gluten free diet. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/2805354/IgA_isotyping_of_antigliadin_antibodies__A_possible_clue_for_a_less_invasive_diagnosis_of_coeliac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0009-8981(89)90362-8 DB - PRIME DP - Unbound Medicine ER -