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Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, Mytilus galloprovincialis, exposed to maltose-stabilised Ag nanoparticles of different sizes.
Nanotoxicology. 2017 03; 11(2):168-183.N

Abstract

Bioavailability and toxicity of maltose-stabilised AgNPs of different sizes (20, 40 and 100 nm) in mussels were compared with bulk and aqueous forms of the metal through a two-tier experimental approach. In the first tier, mussels were exposed for 3 d to a range of concentrations (0.75, 75, 750 μg Ag/l) in the form of Ag20-Mal, Ag40-Mal, Ag100-Mal, bulk Ag and aqueous Ag (as AgNO3), as well as to the concentrations of maltose used in the formulation of NPs. Mortality, bioaccumulation, tissue and cell distribution and lysosomal responses were investigated. In the second tier, mussels were exposed for 21 d to Ag20-Mal, Ag100-Mal, bulk Ag and aqueous Ag at the lowest effective concentration selected after Tier 1 (0.75 μg Ag/l), biomarkers and toxicopathic effects were investigated. Aqueous Ag was lethal within 3 d at 75 μg Ag/l; Ag NPs or bulk Ag did not produce significant mortality at 750 μg Ag/l. Ag accumulation was limited and metallothionein gene transcription was not regulated although metal accumulation occurred in digestive, brown and stomach epithelial cells and in gut lumen after exposure to AgNPs and aqueous Ag starting at low concentrations after 1 d. Electrondense particles (<10 nm) in lysosomes and residual bodies after exposure to AgNPs contained Ag and S (X-ray). Intralysosomal metal accumulation and lysosomal membrane destabilisation were enhanced after exposure to all the forms of Ag and more marked after exposure to Ag20-Mal than to larger NPs. 21 d exposure to AgNPs provoked digestive cell loss and loss of digestive gland integrity, resulting in atrophy-necrosis in digestive alveoli and oedema/hyperplasia in gills (Ag NP), vacuolisation in digestive cells (aqueous Ag) and haemocyte infiltration of connective tissue (all treatments). Intralysosomal metal accumulation, lysosomal responses and toxicopathic effects are enhanced at decreasing sizes and appear to be caused by Ag+ ions released from NPs, although the metal was not substantially accumulated.

Authors+Show Affiliations

a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.a CBET Research Group, Dept. Zoology and Animal Cell Biology, Faculty of Science and Technology , Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country , Basque Country , Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28055263

Citation

Jimeno-Romero, A, et al. "Digestive Cell Lysosomes as Main Targets for Ag Accumulation and Toxicity in Marine Mussels, Mytilus Galloprovincialis, Exposed to Maltose-stabilised Ag Nanoparticles of Different Sizes." Nanotoxicology, vol. 11, no. 2, 2017, pp. 168-183.
Jimeno-Romero A, Bilbao E, Izagirre U, et al. Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, Mytilus galloprovincialis, exposed to maltose-stabilised Ag nanoparticles of different sizes. Nanotoxicology. 2017;11(2):168-183.
Jimeno-Romero, A., Bilbao, E., Izagirre, U., Cajaraville, M. P., Marigómez, I., & Soto, M. (2017). Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, Mytilus galloprovincialis, exposed to maltose-stabilised Ag nanoparticles of different sizes. Nanotoxicology, 11(2), 168-183. https://doi.org/10.1080/17435390.2017.1279358
Jimeno-Romero A, et al. Digestive Cell Lysosomes as Main Targets for Ag Accumulation and Toxicity in Marine Mussels, Mytilus Galloprovincialis, Exposed to Maltose-stabilised Ag Nanoparticles of Different Sizes. Nanotoxicology. 2017;11(2):168-183. PubMed PMID: 28055263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, Mytilus galloprovincialis, exposed to maltose-stabilised Ag nanoparticles of different sizes. AU - Jimeno-Romero,A, AU - Bilbao,E, AU - Izagirre,U, AU - Cajaraville,M P, AU - Marigómez,I, AU - Soto,M, Y1 - 2017/01/24/ PY - 2017/1/6/pubmed PY - 2017/6/27/medline PY - 2017/1/6/entrez KW - Ag NPs KW - biomarkers KW - digestive gland KW - gills KW - histopathology KW - metal form KW - nanosize KW - toxicity SP - 168 EP - 183 JF - Nanotoxicology JO - Nanotoxicology VL - 11 IS - 2 N2 - Bioavailability and toxicity of maltose-stabilised AgNPs of different sizes (20, 40 and 100 nm) in mussels were compared with bulk and aqueous forms of the metal through a two-tier experimental approach. In the first tier, mussels were exposed for 3 d to a range of concentrations (0.75, 75, 750 μg Ag/l) in the form of Ag20-Mal, Ag40-Mal, Ag100-Mal, bulk Ag and aqueous Ag (as AgNO3), as well as to the concentrations of maltose used in the formulation of NPs. Mortality, bioaccumulation, tissue and cell distribution and lysosomal responses were investigated. In the second tier, mussels were exposed for 21 d to Ag20-Mal, Ag100-Mal, bulk Ag and aqueous Ag at the lowest effective concentration selected after Tier 1 (0.75 μg Ag/l), biomarkers and toxicopathic effects were investigated. Aqueous Ag was lethal within 3 d at 75 μg Ag/l; Ag NPs or bulk Ag did not produce significant mortality at 750 μg Ag/l. Ag accumulation was limited and metallothionein gene transcription was not regulated although metal accumulation occurred in digestive, brown and stomach epithelial cells and in gut lumen after exposure to AgNPs and aqueous Ag starting at low concentrations after 1 d. Electrondense particles (<10 nm) in lysosomes and residual bodies after exposure to AgNPs contained Ag and S (X-ray). Intralysosomal metal accumulation and lysosomal membrane destabilisation were enhanced after exposure to all the forms of Ag and more marked after exposure to Ag20-Mal than to larger NPs. 21 d exposure to AgNPs provoked digestive cell loss and loss of digestive gland integrity, resulting in atrophy-necrosis in digestive alveoli and oedema/hyperplasia in gills (Ag NP), vacuolisation in digestive cells (aqueous Ag) and haemocyte infiltration of connective tissue (all treatments). Intralysosomal metal accumulation, lysosomal responses and toxicopathic effects are enhanced at decreasing sizes and appear to be caused by Ag+ ions released from NPs, although the metal was not substantially accumulated. SN - 1743-5404 UR - https://www.unboundmedicine.com/medline/citation/28055263/Digestive_cell_lysosomes_as_main_targets_for_Ag_accumulation_and_toxicity_in_marine_mussels_Mytilus_galloprovincialis_exposed_to_maltose_stabilised_Ag_nanoparticles_of_different_sizes_ L2 - https://www.tandfonline.com/doi/full/10.1080/17435390.2017.1279358 DB - PRIME DP - Unbound Medicine ER -