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Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae).
BMC Complement Altern Med. 2017 Jan 07; 17(1):28.BC

Abstract

BACKGROUND

Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae) is used in Cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. In this study, the methanol extract from the leaves (EDL), fractions (EDLa-d), sub-fractions EDLc1-7 and EDLc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of Gram-negative and Gram-positive bacteria including multidrug resistant (MDR) phenotypes.

METHODS

The broth microdilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples; column chromatography was used for the fractionation and purification of the leaves extract whilst the chemical structures of compounds were determined using spectroscopic techniques.

RESULTS

Phytochemical investigation lead to the isolation of a mixture (1:3) of stigmasterol and β-sitosterol (1 + 2), euphol (3), sitosterol-O-β- D -xylopyranoside (4), 3,3',4'-tri-O-methylellagic acid (5), a mixture (1:1) of afzelin and quercetin-3-O-β-D-xylopyranoside (6 + 7), 3,3',4'-tri-O-methylellagic acid 4-O-β-D-glucopyranoside (8), ellagic acid-4-O-β-xylopyranoside-3,3',4'-trimethyl ether (9) from EDLc. Crude extract and fractions displayed selective activities with MIC values ranged from 32 to 1024 μg/mL for EDL against 84.9% of the 33 tested bacteria, 93.9% for EDLc, 69.7% for EDLb, 33.4% for EDLa and 0.03% for EDLd. MIC values ranged from 16 to 1024 μg/mL were obtained with EDLc3 and EDLc4 on all tested bacteria meanwhile other sub-fractions displayed selective activities. MIC value of 32 μg/mL was obtained with fractions EDLa against Escherichia coli AG100, EDLc against Enterobacer aerogenes ATCC13048 and EA298. For sub-fractions obtained from EDLc, the lowest MIC value of 16 μg/mL was recorded with EDLc3 against Staphylococcus aureus MRSA11. A corresponding value of 8 μg/mL against Providencia stuartii NAE16 was recorded with EDLc33 obtained from further fractionation of EDLc3. EDLc3 had MIC values below 100 μg/mL against all tested bacteria. Compound 5 as well as the mixture (1:1) of 6 and 7 inhibited the growth of all the tested bacteria with MICs ranged from 64 to 256 μg/mL.

CONCLUSION

Elaeophorbia drupifera is a potential source of phytomedicine to tackle MDR bacteria. Sub-fraction EDLc3 was more active than all isolated compounds and deserves further investigations to develop natural drug to combat Gram-negative, Gram-positive bacteria and otherwise MDR phenotypes.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina, Florianópolis, 88040-900, Santa Catarina, Brazil.Department of Chemistry, Faculty of Science, Anadolu University, Eskişehir, Turkey.Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaoundé, Cameroon.Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. kuetevictor@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28061888

Citation

Voukeng, Igor K., et al. "Antibacterial Activities of the Methanol Extract, Fractions and Compounds From Elaeophorbia Drupifera (Thonn.) Stapf. (Euphorbiaceae)." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 28.
Voukeng IK, Nganou BK, Sandjo LP, et al. Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae). BMC Complement Altern Med. 2017;17(1):28.
Voukeng, I. K., Nganou, B. K., Sandjo, L. P., Celik, I., Beng, V. P., Tane, P., & Kuete, V. (2017). Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae). BMC Complementary and Alternative Medicine, 17(1), 28. https://doi.org/10.1186/s12906-016-1509-y
Voukeng IK, et al. Antibacterial Activities of the Methanol Extract, Fractions and Compounds From Elaeophorbia Drupifera (Thonn.) Stapf. (Euphorbiaceae). BMC Complement Altern Med. 2017 Jan 7;17(1):28. PubMed PMID: 28061888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae). AU - Voukeng,Igor K, AU - Nganou,Blaise K, AU - Sandjo,Louis P, AU - Celik,Ilhami, AU - Beng,Veronique P, AU - Tane,Pierre, AU - Kuete,Victor, Y1 - 2017/01/07/ PY - 2016/08/26/received PY - 2016/12/02/accepted PY - 2017/1/8/entrez PY - 2017/1/8/pubmed PY - 2017/1/28/medline KW - Antibacterial KW - Compounds KW - Crude extract KW - Elaeophorbia drupifera KW - Fractions KW - Multi-drug resistance SP - 28 EP - 28 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae) is used in Cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. In this study, the methanol extract from the leaves (EDL), fractions (EDLa-d), sub-fractions EDLc1-7 and EDLc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of Gram-negative and Gram-positive bacteria including multidrug resistant (MDR) phenotypes. METHODS: The broth microdilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples; column chromatography was used for the fractionation and purification of the leaves extract whilst the chemical structures of compounds were determined using spectroscopic techniques. RESULTS: Phytochemical investigation lead to the isolation of a mixture (1:3) of stigmasterol and β-sitosterol (1 + 2), euphol (3), sitosterol-O-β- D -xylopyranoside (4), 3,3',4'-tri-O-methylellagic acid (5), a mixture (1:1) of afzelin and quercetin-3-O-β-D-xylopyranoside (6 + 7), 3,3',4'-tri-O-methylellagic acid 4-O-β-D-glucopyranoside (8), ellagic acid-4-O-β-xylopyranoside-3,3',4'-trimethyl ether (9) from EDLc. Crude extract and fractions displayed selective activities with MIC values ranged from 32 to 1024 μg/mL for EDL against 84.9% of the 33 tested bacteria, 93.9% for EDLc, 69.7% for EDLb, 33.4% for EDLa and 0.03% for EDLd. MIC values ranged from 16 to 1024 μg/mL were obtained with EDLc3 and EDLc4 on all tested bacteria meanwhile other sub-fractions displayed selective activities. MIC value of 32 μg/mL was obtained with fractions EDLa against Escherichia coli AG100, EDLc against Enterobacer aerogenes ATCC13048 and EA298. For sub-fractions obtained from EDLc, the lowest MIC value of 16 μg/mL was recorded with EDLc3 against Staphylococcus aureus MRSA11. A corresponding value of 8 μg/mL against Providencia stuartii NAE16 was recorded with EDLc33 obtained from further fractionation of EDLc3. EDLc3 had MIC values below 100 μg/mL against all tested bacteria. Compound 5 as well as the mixture (1:1) of 6 and 7 inhibited the growth of all the tested bacteria with MICs ranged from 64 to 256 μg/mL. CONCLUSION: Elaeophorbia drupifera is a potential source of phytomedicine to tackle MDR bacteria. Sub-fraction EDLc3 was more active than all isolated compounds and deserves further investigations to develop natural drug to combat Gram-negative, Gram-positive bacteria and otherwise MDR phenotypes. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28061888/Antibacterial_activities_of_the_methanol_extract_fractions_and_compounds_from_Elaeophorbia_drupifera__Thonn___Stapf___Euphorbiaceae__ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1509-y DB - PRIME DP - Unbound Medicine ER -