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Proteomic analysis to unravel the complex venom proteome of eastern India Naja naja: Correlation of venom composition with its biochemical and pharmacological properties.
J Proteomics. 2017 03 06; 156:29-39.JP

Abstract

The complex venom proteome of the eastern India (EI) spectacled cobra (Naja naja) was analyzed using tandem mass spectrometry of cation-exchange venom fractions. About 75% of EI N. naja venom proteins were <18kDa and cationic at physiological pH of blood. SDS-PAGE (non-reduced) analysis indicated that in the native state venom proteins either interacted with each-other or self-aggregated resulting in the formation of higher molecular mass complexes. Proteomic analysis revealed that 43 enzymatic and non-enzymatic proteins in EI N. naja venom with a percent composition of about 28.4% and 71.6% respectively were distributed over 15 venom protein families. The three finger toxins (63.8%) and phospholipase A2s (11.4%) were the most abundant families of non-enzymatic and enzymatic proteins, respectively. nanoLC-ESI-MS/MS analysis demonstrated the occurrence of acetylcholinesterase, phosphodiesterase, cholinesterase and snake venom serine proteases in N. naja venom previously not detected by proteomic analysis. ATPase, ADPase, hyaluronidase, TAME, and BAEE-esterase activities were detected by biochemical analysis; however, due to a limitation in the protein database depository they were not identified in EI N. naja venom by proteomic analysis. The proteome composition of EI N. naja venom was well correlated with its in vitro and in vivo pharmacological properties in experimental animals and envenomed human.

BIOLOGICAL SIGNIFICANCE

Proteomic analysis reveals the complex and diverse protein profile of EI N. naja venom which collectively contributes to the severe pathophysiological manifestation upon cobra envenomation. The study has also aided in comprehending the compositional variation in venom proteins of N. naja within the Indian sub-continent. In addition, this study has also identified several enzymes in EI N. naja venom which were previously uncharacterized by proteomic analysis of Naja venom.

Authors+Show Affiliations

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India. Electronic address: akm@tezu.ernet.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28062377

Citation

Dutta, Sumita, et al. "Proteomic Analysis to Unravel the Complex Venom Proteome of Eastern India Naja Naja: Correlation of Venom Composition With Its Biochemical and Pharmacological Properties." Journal of Proteomics, vol. 156, 2017, pp. 29-39.
Dutta S, Chanda A, Kalita B, et al. Proteomic analysis to unravel the complex venom proteome of eastern India Naja naja: Correlation of venom composition with its biochemical and pharmacological properties. J Proteomics. 2017;156:29-39.
Dutta, S., Chanda, A., Kalita, B., Islam, T., Patra, A., & Mukherjee, A. K. (2017). Proteomic analysis to unravel the complex venom proteome of eastern India Naja naja: Correlation of venom composition with its biochemical and pharmacological properties. Journal of Proteomics, 156, 29-39. https://doi.org/10.1016/j.jprot.2016.12.018
Dutta S, et al. Proteomic Analysis to Unravel the Complex Venom Proteome of Eastern India Naja Naja: Correlation of Venom Composition With Its Biochemical and Pharmacological Properties. J Proteomics. 2017 03 6;156:29-39. PubMed PMID: 28062377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis to unravel the complex venom proteome of eastern India Naja naja: Correlation of venom composition with its biochemical and pharmacological properties. AU - Dutta,Sumita, AU - Chanda,Abhishek, AU - Kalita,Bhargab, AU - Islam,Taufikul, AU - Patra,Aparup, AU - Mukherjee,Ashis K, Y1 - 2017/01/04/ PY - 2016/08/18/received PY - 2016/12/27/revised PY - 2016/12/29/accepted PY - 2017/1/8/pubmed PY - 2017/11/29/medline PY - 2017/1/8/entrez KW - Acetylcholinesterase KW - LC-MS/MS KW - Pathophysiology KW - Phospholipase A(2) KW - Tandem mass spectrometry KW - Three-finger toxins KW - Venomics SP - 29 EP - 39 JF - Journal of proteomics JO - J Proteomics VL - 156 N2 - : The complex venom proteome of the eastern India (EI) spectacled cobra (Naja naja) was analyzed using tandem mass spectrometry of cation-exchange venom fractions. About 75% of EI N. naja venom proteins were <18kDa and cationic at physiological pH of blood. SDS-PAGE (non-reduced) analysis indicated that in the native state venom proteins either interacted with each-other or self-aggregated resulting in the formation of higher molecular mass complexes. Proteomic analysis revealed that 43 enzymatic and non-enzymatic proteins in EI N. naja venom with a percent composition of about 28.4% and 71.6% respectively were distributed over 15 venom protein families. The three finger toxins (63.8%) and phospholipase A2s (11.4%) were the most abundant families of non-enzymatic and enzymatic proteins, respectively. nanoLC-ESI-MS/MS analysis demonstrated the occurrence of acetylcholinesterase, phosphodiesterase, cholinesterase and snake venom serine proteases in N. naja venom previously not detected by proteomic analysis. ATPase, ADPase, hyaluronidase, TAME, and BAEE-esterase activities were detected by biochemical analysis; however, due to a limitation in the protein database depository they were not identified in EI N. naja venom by proteomic analysis. The proteome composition of EI N. naja venom was well correlated with its in vitro and in vivo pharmacological properties in experimental animals and envenomed human. BIOLOGICAL SIGNIFICANCE: Proteomic analysis reveals the complex and diverse protein profile of EI N. naja venom which collectively contributes to the severe pathophysiological manifestation upon cobra envenomation. The study has also aided in comprehending the compositional variation in venom proteins of N. naja within the Indian sub-continent. In addition, this study has also identified several enzymes in EI N. naja venom which were previously uncharacterized by proteomic analysis of Naja venom. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/28062377/Proteomic_analysis_to_unravel_the_complex_venom_proteome_of_eastern_India_Naja_naja:_Correlation_of_venom_composition_with_its_biochemical_and_pharmacological_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(16)30546-2 DB - PRIME DP - Unbound Medicine ER -