Tags

Type your tag names separated by a space and hit enter

Diabetic and idiopathic gastroparesis is associated with loss of CD206-positive macrophages in the gastric antrum.
Neurogastroenterol Motil. 2017 Jun; 29(6)NM

Abstract

BACKGROUND

Animal studies have increasingly highlighted the role of macrophages in the development of delayed gastric emptying. However, their role in the pathophysiology of human gastroparesis is unclear. Our aim was to determine changes in macrophages and other cell types in the gastric antrum muscularis propria of patients with diabetic and idiopathic gastroparesis.

METHODS

Full thickness gastric antrum biopsies were obtained from patients enrolled in the Gastroparesis Clinical Research Consortium (11 diabetic, 6 idiopathic) and 5 controls. Immunolabeling and quantitative assessment was done for interstitial cells of Cajal (ICC) (Kit), enteric nerves protein gene product 9.5, neuronal nitric oxide synthase, vasoactive intestinal peptide, substance P, tyrosine hydroxylase), overall immune cells (CD45) and anti-inflammatory macrophages (CD206). Gastric emptying was assessed using nuclear medicine scintigraphy and symptom severity using the Gastroparesis Cardinal Symptom Index.

RESULTS

Both diabetic and idiopathic gastroparesis patients showed loss of ICC as compared to controls (Mean [standard error of mean]/hpf: diabetic, 2.28 [0.16]; idiopathic, 2.53 [0.47]; controls, 6.05 [0.62]; P=.004). Overall immune cell population (CD45) was unchanged but there was a loss of anti-inflammatory macrophages (CD206) in circular muscle (diabetic, 3.87 [0.32]; idiopathic, 4.16 [0.52]; controls, 6.59 [1.09]; P=.04) and myenteric plexus (diabetic, 3.83 [0.27]; idiopathic, 3.59 [0.68]; controls, 7.46 [0.51]; P=.004). There was correlation between the number of ICC and CD206-positive cells (r=.55, P=.008). Enteric nerves (PGP9.5) were unchanged: diabetic, 33.64 (3.45); idiopathic, 41.26 (6.40); controls, 46.80 (6.04).

CONCLUSION

Loss of antral CD206-positive anti-inflammatory macrophages is a key feature in human gastroparesis and it is associates with ICC loss.

Authors+Show Affiliations

Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.Johns Hopkins School of Medicine, Baltimore, MD, USA.Temple University, Philadelphia, PA, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.Johns Hopkins University Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.Wake Forest University, Winston-Salem, NC, USA.Texas Tech University, El Paso, TX, USA.Texas Tech University, El Paso, TX, USA.University of Michigan, Ann Arbor, MI, USA.Stanford University, Palo Alto, CA, USA.University of Louisville, Louisville, KY, USA.California Pacific Medical Center, San Francisco, CA, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA.Enteric NeuroScience Program, Mayo Clinic, Rochester, MN, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28066953

Citation

Grover, M, et al. "Diabetic and Idiopathic Gastroparesis Is Associated With Loss of CD206-positive Macrophages in the Gastric Antrum." Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, vol. 29, no. 6, 2017.
Grover M, Bernard CE, Pasricha PJ, et al. Diabetic and idiopathic gastroparesis is associated with loss of CD206-positive macrophages in the gastric antrum. Neurogastroenterol Motil. 2017;29(6).
Grover, M., Bernard, C. E., Pasricha, P. J., Parkman, H. P., Gibbons, S. J., Tonascia, J., Koch, K. L., McCallum, R. W., Sarosiek, I., Hasler, W. L., Nguyen, L. A. B., Abell, T. L., Snape, W. J., Kendrick, M. L., Kellogg, T. A., McKenzie, T. J., Hamilton, F. A., & Farrugia, G. (2017). Diabetic and idiopathic gastroparesis is associated with loss of CD206-positive macrophages in the gastric antrum. Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, 29(6). https://doi.org/10.1111/nmo.13018
Grover M, et al. Diabetic and Idiopathic Gastroparesis Is Associated With Loss of CD206-positive Macrophages in the Gastric Antrum. Neurogastroenterol Motil. 2017;29(6) PubMed PMID: 28066953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diabetic and idiopathic gastroparesis is associated with loss of CD206-positive macrophages in the gastric antrum. AU - Grover,M, AU - Bernard,C E, AU - Pasricha,P J, AU - Parkman,H P, AU - Gibbons,S J, AU - Tonascia,J, AU - Koch,K L, AU - McCallum,R W, AU - Sarosiek,I, AU - Hasler,W L, AU - Nguyen,L A B, AU - Abell,T L, AU - Snape,W J, AU - Kendrick,M L, AU - Kellogg,T A, AU - McKenzie,T J, AU - Hamilton,F A, AU - Farrugia,G, AU - ,, Y1 - 2017/01/09/ PY - 2016/09/07/received PY - 2016/11/30/accepted PY - 2017/1/10/pubmed PY - 2018/3/6/medline PY - 2017/1/10/entrez KW - enteric nervous system KW - gastrointestinal motility KW - immune cells KW - interstitial cells of Cajal KW - macrophages JF - Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society JO - Neurogastroenterol Motil VL - 29 IS - 6 N2 - BACKGROUND: Animal studies have increasingly highlighted the role of macrophages in the development of delayed gastric emptying. However, their role in the pathophysiology of human gastroparesis is unclear. Our aim was to determine changes in macrophages and other cell types in the gastric antrum muscularis propria of patients with diabetic and idiopathic gastroparesis. METHODS: Full thickness gastric antrum biopsies were obtained from patients enrolled in the Gastroparesis Clinical Research Consortium (11 diabetic, 6 idiopathic) and 5 controls. Immunolabeling and quantitative assessment was done for interstitial cells of Cajal (ICC) (Kit), enteric nerves protein gene product 9.5, neuronal nitric oxide synthase, vasoactive intestinal peptide, substance P, tyrosine hydroxylase), overall immune cells (CD45) and anti-inflammatory macrophages (CD206). Gastric emptying was assessed using nuclear medicine scintigraphy and symptom severity using the Gastroparesis Cardinal Symptom Index. RESULTS: Both diabetic and idiopathic gastroparesis patients showed loss of ICC as compared to controls (Mean [standard error of mean]/hpf: diabetic, 2.28 [0.16]; idiopathic, 2.53 [0.47]; controls, 6.05 [0.62]; P=.004). Overall immune cell population (CD45) was unchanged but there was a loss of anti-inflammatory macrophages (CD206) in circular muscle (diabetic, 3.87 [0.32]; idiopathic, 4.16 [0.52]; controls, 6.59 [1.09]; P=.04) and myenteric plexus (diabetic, 3.83 [0.27]; idiopathic, 3.59 [0.68]; controls, 7.46 [0.51]; P=.004). There was correlation between the number of ICC and CD206-positive cells (r=.55, P=.008). Enteric nerves (PGP9.5) were unchanged: diabetic, 33.64 (3.45); idiopathic, 41.26 (6.40); controls, 46.80 (6.04). CONCLUSION: Loss of antral CD206-positive anti-inflammatory macrophages is a key feature in human gastroparesis and it is associates with ICC loss. SN - 1365-2982 UR - https://www.unboundmedicine.com/medline/citation/28066953/Diabetic_and_idiopathic_gastroparesis_is_associated_with_loss_of_CD206_positive_macrophages_in_the_gastric_antrum_ L2 - https://doi.org/10.1111/nmo.13018 DB - PRIME DP - Unbound Medicine ER -