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Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells.
Int J Mol Sci. 2017 Jan 05; 18(1)IJ

Abstract

Sailuotong (SLT) is a standardised three-herb formulation consisting of Panax ginseng, Ginkgo biloba, and Crocus sativus designed for the management of vascular dementia. While the latest clinical trials have demonstrated beneficial effects of SLT in vascular dementia, the underlying cellular mechanisms have not been fully explored. The aim of this study was to assess the ability and mechanisms of SLT to act against hydrogen peroxide (H₂O₂)-induced oxidative damage in cultured human vascular endothelial cells (EAhy926). SLT (1-50 µg/mL) significantly suppressed the H₂O₂-induced cell death and abolished the H₂O₂-induced reactive oxygen species (ROS) generation in a concentration-dependent manner. Similarly, H₂O₂ (0.5 mM; 24 h) caused a ~2-fold increase in lactate dehydrogenase (LDH) release from the EA.hy926 cells which were significantly suppressed by SLT (1-50 µg/mL) in a concentration-dependent manner. Incubation of SLT (50 µg/mL) increased superoxide dismutase (SOD) activity and suppressed the H₂O₂-enhanced Bax/Bcl-2 ratio and cleaved caspase-3 expression. In conclusion, our results suggest that SLT protects EA.hy916 cells against H₂O₂-mediated injury via direct reduction of intracellular ROS generation and an increase in SOD activity. These protective effects are closely associated with the inhibition of the apoptotic death cascade via the suppression of caspase-3 activation and reduction of Bax/Bcl-2 ratio, thereby indicating a potential mechanism of action for the clinical effects observed.

Authors+Show Affiliations

National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. s.seto@westernsydeny.edu.au.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. d.chang@westernsydney.edu.au.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. i.ko@westernsydney.edu.au.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. P.Zhou@westernsydney.edu.au.Faculty of Medicine, University of New South Wales, NSW 2052, Australia. hosen.kiat@chi.org.au. School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia. hosen.kiat@chi.org.au. Faculty of Medicine and Health Sciences, Macquarie University, NSW 2109, Australia. hosen.kiat@chi.org.au.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. A.Bensoussan@westernsydney.edu.au.State Key Laboratory Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China. simonlee@umac.mo.State Key Laboratory Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China. maghoi@umac.mo.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. G.Steiner@westernsydney.edu.au.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia. Jianxun.Liu@westernsydney.edu.au. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. Jianxun.Liu@westernsydney.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28067784

Citation

Seto, Sai Wang, et al. "Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells." International Journal of Molecular Sciences, vol. 18, no. 1, 2017.
Seto SW, Chang D, Ko WM, et al. Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells. Int J Mol Sci. 2017;18(1).
Seto, S. W., Chang, D., Ko, W. M., Zhou, X., Kiat, H., Bensoussan, A., Lee, S. M., Hoi, M. P., Steiner, G. Z., & Liu, J. (2017). Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells. International Journal of Molecular Sciences, 18(1). https://doi.org/10.3390/ijms18010095
Seto SW, et al. Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells. Int J Mol Sci. 2017 Jan 5;18(1) PubMed PMID: 28067784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sailuotong Prevents Hydrogen Peroxide (H₂O₂)-Induced Injury in EA.hy926 Cells. AU - Seto,Sai Wang, AU - Chang,Dennis, AU - Ko,Wai Man, AU - Zhou,Xian, AU - Kiat,Hosen, AU - Bensoussan,Alan, AU - Lee,Simon M Y, AU - Hoi,Maggie P M, AU - Steiner,Genevieve Z, AU - Liu,Jianxun, Y1 - 2017/01/05/ PY - 2016/10/13/received PY - 2016/12/15/revised PY - 2016/12/22/accepted PY - 2017/1/10/entrez PY - 2017/1/10/pubmed PY - 2017/4/30/medline KW - Sailuotong (SLT) KW - apoptosis KW - endothelial dysfunction KW - herbal medicine KW - reactive oxygen species KW - vascular dementia JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 1 N2 - Sailuotong (SLT) is a standardised three-herb formulation consisting of Panax ginseng, Ginkgo biloba, and Crocus sativus designed for the management of vascular dementia. While the latest clinical trials have demonstrated beneficial effects of SLT in vascular dementia, the underlying cellular mechanisms have not been fully explored. The aim of this study was to assess the ability and mechanisms of SLT to act against hydrogen peroxide (H₂O₂)-induced oxidative damage in cultured human vascular endothelial cells (EAhy926). SLT (1-50 µg/mL) significantly suppressed the H₂O₂-induced cell death and abolished the H₂O₂-induced reactive oxygen species (ROS) generation in a concentration-dependent manner. Similarly, H₂O₂ (0.5 mM; 24 h) caused a ~2-fold increase in lactate dehydrogenase (LDH) release from the EA.hy926 cells which were significantly suppressed by SLT (1-50 µg/mL) in a concentration-dependent manner. Incubation of SLT (50 µg/mL) increased superoxide dismutase (SOD) activity and suppressed the H₂O₂-enhanced Bax/Bcl-2 ratio and cleaved caspase-3 expression. In conclusion, our results suggest that SLT protects EA.hy916 cells against H₂O₂-mediated injury via direct reduction of intracellular ROS generation and an increase in SOD activity. These protective effects are closely associated with the inhibition of the apoptotic death cascade via the suppression of caspase-3 activation and reduction of Bax/Bcl-2 ratio, thereby indicating a potential mechanism of action for the clinical effects observed. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28067784/Sailuotong_Prevents_Hydrogen_Peroxide__H₂O₂__Induced_Injury_in_EA_hy926_Cells_ L2 - http://www.mdpi.com/resolver?pii=ijms18010095 DB - PRIME DP - Unbound Medicine ER -