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RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart.
Phytochem Anal. 2017 May; 28(3):217-229.PA

Abstract

INTRODUCTION

Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition.

OBJECTIVE

That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses. Its comparison with by-products derived from leaves was also studied.

METHODOLOGY

Hydromethanol extracts from wood and leaves with stems of 'Chemlali' olive cultivar were analysed using reversed-phase (RP) high-performance liquid chromatography (HPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) in negative ion mode. Tandem MS experiments were performed to establish the chemical structure of olive phytochemicals.

RESULTS

A total of 85 compounds were characterised in the studied olive parts and classified as: sugars (3), organic acids (5), one phenolic aldehyde, simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14), coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5), secoiridoids (32), and lignans (5). To our knowledge, the major part of these metabolites was not previously reported in olive tree wood, and 10 olive chemical constituents were identified for the first time in the Oleaceae family.

CONCLUSION

The results presented here demonstrated the usefulness of the methodology proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an exhaustive metabolic profiling and to recover new biologically active compounds in olive wood with pharmacologic and cosmetic potential. Copyright © 2017 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain. Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain. Department of Analytical Chemistry, University of Cordoba, Annex C-3 Building, Campus of Rabanales, 14071, Córdoba, Spain.Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28067965

Citation

Ammar, Sonda, et al. "RP-HPLC-DAD-ESI-QTOF-MS Based Metabolic Profiling of the Potential Olea Europaea By-product "wood" and Its Comparison With Leaf Counterpart." Phytochemical Analysis : PCA, vol. 28, no. 3, 2017, pp. 217-229.
Ammar S, Contreras MDM, Gargouri B, et al. RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart. Phytochem Anal. 2017;28(3):217-229.
Ammar, S., Contreras, M. D. M., Gargouri, B., Segura-Carretero, A., & Bouaziz, M. (2017). RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart. Phytochemical Analysis : PCA, 28(3), 217-229. https://doi.org/10.1002/pca.2664
Ammar S, et al. RP-HPLC-DAD-ESI-QTOF-MS Based Metabolic Profiling of the Potential Olea Europaea By-product "wood" and Its Comparison With Leaf Counterpart. Phytochem Anal. 2017;28(3):217-229. PubMed PMID: 28067965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart. AU - Ammar,Sonda, AU - Contreras,Maria Del Mar, AU - Gargouri,Boutheina, AU - Segura-Carretero,Antonio, AU - Bouaziz,Mohamed, Y1 - 2017/01/09/ PY - 2016/08/21/received PY - 2016/10/15/revised PY - 2016/10/24/accepted PY - 2017/1/10/pubmed PY - 2017/8/9/medline PY - 2017/1/10/entrez KW - olive leaves KW - olive wood KW - phytochemicals KW - secoiridoids KW - tandem mass spectrometry SP - 217 EP - 229 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 28 IS - 3 N2 - INTRODUCTION: Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition. OBJECTIVE: That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses. Its comparison with by-products derived from leaves was also studied. METHODOLOGY: Hydromethanol extracts from wood and leaves with stems of 'Chemlali' olive cultivar were analysed using reversed-phase (RP) high-performance liquid chromatography (HPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) in negative ion mode. Tandem MS experiments were performed to establish the chemical structure of olive phytochemicals. RESULTS: A total of 85 compounds were characterised in the studied olive parts and classified as: sugars (3), organic acids (5), one phenolic aldehyde, simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14), coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5), secoiridoids (32), and lignans (5). To our knowledge, the major part of these metabolites was not previously reported in olive tree wood, and 10 olive chemical constituents were identified for the first time in the Oleaceae family. CONCLUSION: The results presented here demonstrated the usefulness of the methodology proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an exhaustive metabolic profiling and to recover new biologically active compounds in olive wood with pharmacologic and cosmetic potential. Copyright © 2017 John Wiley & Sons, Ltd. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/28067965/RP_HPLC_DAD_ESI_QTOF_MS_based_metabolic_profiling_of_the_potential_Olea_europaea_by_product_"wood"_and_its_comparison_with_leaf_counterpart_ DB - PRIME DP - Unbound Medicine ER -