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Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β-Cyclodextrin.
J Food Sci. 2017 Feb; 82(2):394-400.JF

Abstract

Use of organic solvents to extract phenolic compounds from plants may result in environmental pollution and cause health problems in persons. Replacing organic extraction solvents by green extracting agents without affecting the extraction yield is one of the most pressing problems to be solved. The aim of this study is to evaluate the capacity of β-cyclodextrin (β-CD) to recover phenolic compounds from tea leaves. The extract obtained using the ethanol/water mixture presented the highest total phenolic content, followed by those obtained using β-CD solution and water. HPLC analysis of the extracts showed that the addition of β-CD to the extracting agent had a selective effect on the extraction of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). The extraction yield of EGCG and ECG using 15 g/L β-CD were higher than that obtained using water and 50% ethanol. Molecular docking results indicated that the molecules of EGCG and ECG were more inclined to interact with β-CD than epigallocatechin, epicatechin, and gallocatechin. The impact of β-CD concentration, temperature, and time on EGCG and ECG extraction from tea leaves was investigated and the maximum amount of EGCG (118.7 mg/g) and ECG (54.6 mg/g) were achieved when extracted with 25 g/L aqueous β-CD solution at 60 °C for 60 min. The present study indicates that aqueous β-CD can be used as an alternative to organic solvents to recover EGCG and ECG from tea leaves.

Authors+Show Affiliations

College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.College of Food Science and Engineering, Northwest A&F Univ., Yangling, Shaanxi, 712100, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28071811

Citation

Cui, Lu, et al. "Extraction of Epigallocatechin Gallate and Epicatechin Gallate From Tea Leaves Using Β-Cyclodextrin." Journal of Food Science, vol. 82, no. 2, 2017, pp. 394-400.
Cui L, Liu Y, Liu T, et al. Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β-Cyclodextrin. J Food Sci. 2017;82(2):394-400.
Cui, L., Liu, Y., Liu, T., Yuan, Y., Yue, T., Cai, R., & Wang, Z. (2017). Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β-Cyclodextrin. Journal of Food Science, 82(2), 394-400. https://doi.org/10.1111/1750-3841.13622
Cui L, et al. Extraction of Epigallocatechin Gallate and Epicatechin Gallate From Tea Leaves Using Β-Cyclodextrin. J Food Sci. 2017;82(2):394-400. PubMed PMID: 28071811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β-Cyclodextrin. AU - Cui,Lu, AU - Liu,Yuxuan, AU - Liu,Ting, AU - Yuan,Yahong, AU - Yue,Tianli, AU - Cai,Rui, AU - Wang,Zhouli, Y1 - 2017/01/10/ PY - 2016/09/23/received PY - 2016/11/27/revised PY - 2016/12/19/accepted PY - 2017/1/11/pubmed PY - 2017/5/4/medline PY - 2017/1/11/entrez KW - epicatechin gallate KW - epigallocatechin gallate KW - extraction KW - tea leaves KW - β-cyclodextrin SP - 394 EP - 400 JF - Journal of food science JO - J Food Sci VL - 82 IS - 2 N2 - Use of organic solvents to extract phenolic compounds from plants may result in environmental pollution and cause health problems in persons. Replacing organic extraction solvents by green extracting agents without affecting the extraction yield is one of the most pressing problems to be solved. The aim of this study is to evaluate the capacity of β-cyclodextrin (β-CD) to recover phenolic compounds from tea leaves. The extract obtained using the ethanol/water mixture presented the highest total phenolic content, followed by those obtained using β-CD solution and water. HPLC analysis of the extracts showed that the addition of β-CD to the extracting agent had a selective effect on the extraction of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). The extraction yield of EGCG and ECG using 15 g/L β-CD were higher than that obtained using water and 50% ethanol. Molecular docking results indicated that the molecules of EGCG and ECG were more inclined to interact with β-CD than epigallocatechin, epicatechin, and gallocatechin. The impact of β-CD concentration, temperature, and time on EGCG and ECG extraction from tea leaves was investigated and the maximum amount of EGCG (118.7 mg/g) and ECG (54.6 mg/g) were achieved when extracted with 25 g/L aqueous β-CD solution at 60 °C for 60 min. The present study indicates that aqueous β-CD can be used as an alternative to organic solvents to recover EGCG and ECG from tea leaves. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/28071811/Extraction_of_Epigallocatechin_Gallate_and_Epicatechin_Gallate_from_Tea_Leaves_Using_β_Cyclodextrin_ L2 - https://doi.org/10.1111/1750-3841.13622 DB - PRIME DP - Unbound Medicine ER -