Tags

Type your tag names separated by a space and hit enter

Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis.
J Assist Reprod Genet. 2017 Mar; 34(3):357-364.JA

Abstract

PURPOSE

The aim of this study was to assess whether the intrafollicular cytokine profile in naturally developed follicles is different in women with endometriosis, possibly explaining the lower reproductive outcome in endometriosis patients.

METHODS

A matched case-control study was conducted at a university-based infertility and endometriosis centre. The study population included 17 patients with laparoscopically and histologically confirmed endometriosis (rAFS stages II-IV), each undergoing one natural cycle IVF (NC-IVF) treatment cycle between 2013 and 2015, and 17 age-matched NC-IVF women without diagnosed endometriosis (control group). Follicular fluid and serum was collected at the time of follicle aspiration. The concentrations of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-15, IL-18, TNF-α) and hormones (testosterone, estradiol, AMH) were determined in follicular fluid and serum by single or multiplexed immunoassay and compared between both groups.

RESULTS

In the follicular fluid, IL-1β and IL-6 showed significantly (P < 0.001 and 0.01, respectively) higher median concentrations in the endometriosis group than in the control group and a tendency towards endometriosis severity (rAFS stage) dependence. The levels of the interleukins detectable in follicular fluid were significantly higher than those in the serum (P < 0.01). Follicular estradiol concentration was lower in severe endometriosis patients than in the control group (P = 0.036). Follicular fluid IL-1β and IL-6 levels were not correlated with estradiol in the same compartment in neither patient group.

CONCLUSIONS

In women with moderate and severe endometrioses, some intrafollicular inflammatory cytokines are upregulated and not correlated with intrafollicular hormone concentrations. This might be due to the inflammatory microenvironment in endometriosis women, affecting follicular function and thereby possibly contributing to the reproductive dysfunction in endometriosis.

Authors+Show Affiliations

Division of Gynaecological Endocrinology and Reproductive Medicine, University Women's hospital, Effingerstrasse 102, 3010, Bern, Switzerland. Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan, China.Division of Gynaecological Endocrinology and Reproductive Medicine, University Women's hospital, Effingerstrasse 102, 3010, Bern, Switzerland.Division of Gynaecological Endocrinology and Reproductive Medicine, University Women's hospital, Effingerstrasse 102, 3010, Bern, Switzerland.Division of Gynaecological Endocrinology and Reproductive Medicine, University Women's hospital, Effingerstrasse 102, 3010, Bern, Switzerland. Michael.vonWolff@insel.ch.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28074436

Citation

Wu, Gengxiang, et al. "Intrafollicular Inflammatory Cytokines but Not Steroid Hormone Concentrations Are Increased in Naturally Matured Follicles of Women With Proven Endometriosis." Journal of Assisted Reproduction and Genetics, vol. 34, no. 3, 2017, pp. 357-364.
Wu G, Bersinger NA, Mueller MD, et al. Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. J Assist Reprod Genet. 2017;34(3):357-364.
Wu, G., Bersinger, N. A., Mueller, M. D., & von Wolff, M. (2017). Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. Journal of Assisted Reproduction and Genetics, 34(3), 357-364. https://doi.org/10.1007/s10815-016-0865-3
Wu G, et al. Intrafollicular Inflammatory Cytokines but Not Steroid Hormone Concentrations Are Increased in Naturally Matured Follicles of Women With Proven Endometriosis. J Assist Reprod Genet. 2017;34(3):357-364. PubMed PMID: 28074436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. AU - Wu,Gengxiang, AU - Bersinger,N A, AU - Mueller,M D, AU - von Wolff,M, Y1 - 2017/01/10/ PY - 2016/08/05/received PY - 2016/12/20/accepted PY - 2017/1/12/pubmed PY - 2017/5/23/medline PY - 2017/1/12/entrez KW - Endometriosis KW - Estradiol KW - Follicular fluid KW - Interleukin KW - Natural cycle IVF SP - 357 EP - 364 JF - Journal of assisted reproduction and genetics JO - J. Assist. Reprod. Genet. VL - 34 IS - 3 N2 - PURPOSE: The aim of this study was to assess whether the intrafollicular cytokine profile in naturally developed follicles is different in women with endometriosis, possibly explaining the lower reproductive outcome in endometriosis patients. METHODS: A matched case-control study was conducted at a university-based infertility and endometriosis centre. The study population included 17 patients with laparoscopically and histologically confirmed endometriosis (rAFS stages II-IV), each undergoing one natural cycle IVF (NC-IVF) treatment cycle between 2013 and 2015, and 17 age-matched NC-IVF women without diagnosed endometriosis (control group). Follicular fluid and serum was collected at the time of follicle aspiration. The concentrations of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-15, IL-18, TNF-α) and hormones (testosterone, estradiol, AMH) were determined in follicular fluid and serum by single or multiplexed immunoassay and compared between both groups. RESULTS: In the follicular fluid, IL-1β and IL-6 showed significantly (P < 0.001 and 0.01, respectively) higher median concentrations in the endometriosis group than in the control group and a tendency towards endometriosis severity (rAFS stage) dependence. The levels of the interleukins detectable in follicular fluid were significantly higher than those in the serum (P < 0.01). Follicular estradiol concentration was lower in severe endometriosis patients than in the control group (P = 0.036). Follicular fluid IL-1β and IL-6 levels were not correlated with estradiol in the same compartment in neither patient group. CONCLUSIONS: In women with moderate and severe endometrioses, some intrafollicular inflammatory cytokines are upregulated and not correlated with intrafollicular hormone concentrations. This might be due to the inflammatory microenvironment in endometriosis women, affecting follicular function and thereby possibly contributing to the reproductive dysfunction in endometriosis. SN - 1573-7330 UR - https://www.unboundmedicine.com/medline/citation/28074436/Intrafollicular_inflammatory_cytokines_but_not_steroid_hormone_concentrations_are_increased_in_naturally_matured_follicles_of_women_with_proven_endometriosis_ L2 - https://doi.org/10.1007/s10815-016-0865-3 DB - PRIME DP - Unbound Medicine ER -