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Sensitization of TRPA1 by Protein Kinase A.
PLoS One. 2017; 12(1):e0170097.Plos

Abstract

The TRPA1 ion channel is expressed in nociceptive (pain-sensitive) somatosensory neurons and is activated by a wide variety of chemical irritants, such as acrolein in smoke or isothiocyanates in mustard. Here, we investigate the enhancement of TRPA1 function caused by inflammatory mediators, which is thought to be important in lung conditions such as asthma and COPD. Protein kinase A is an important kinase acting downstream of inflammatory mediators to cause sensitization of TRPA1. By using site-directed mutagenesis, patch-clamp electrophysiology and calcium imaging we identify four amino acid residues, S86, S317, S428, and S972, as the principal targets of PKA-mediated phosphorylation and sensitization of TRPA1.

Authors+Show Affiliations

Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom. Institute of Physiology, Uniklinik RWTH Aachen, Aachen, Germany.Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom. Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany. Center for Physiology and Pharmacology, Medical University Wien, Wien, Austria.Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom. Wolfson Centre for Age-Related Diseases, King's College London, London, United Kingdom.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28076424

Citation

Meents, Jannis E., et al. "Sensitization of TRPA1 By Protein Kinase A." PloS One, vol. 12, no. 1, 2017, pp. e0170097.
Meents JE, Fischer MJ, McNaughton PA. Sensitization of TRPA1 by Protein Kinase A. PLoS One. 2017;12(1):e0170097.
Meents, J. E., Fischer, M. J., & McNaughton, P. A. (2017). Sensitization of TRPA1 by Protein Kinase A. PloS One, 12(1), e0170097. https://doi.org/10.1371/journal.pone.0170097
Meents JE, Fischer MJ, McNaughton PA. Sensitization of TRPA1 By Protein Kinase A. PLoS One. 2017;12(1):e0170097. PubMed PMID: 28076424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitization of TRPA1 by Protein Kinase A. AU - Meents,Jannis E, AU - Fischer,Michael J M, AU - McNaughton,Peter A, Y1 - 2017/01/11/ PY - 2016/10/11/received PY - 2016/12/28/accepted PY - 2017/1/12/entrez PY - 2017/1/12/pubmed PY - 2017/8/11/medline SP - e0170097 EP - e0170097 JF - PloS one JO - PLoS One VL - 12 IS - 1 N2 - The TRPA1 ion channel is expressed in nociceptive (pain-sensitive) somatosensory neurons and is activated by a wide variety of chemical irritants, such as acrolein in smoke or isothiocyanates in mustard. Here, we investigate the enhancement of TRPA1 function caused by inflammatory mediators, which is thought to be important in lung conditions such as asthma and COPD. Protein kinase A is an important kinase acting downstream of inflammatory mediators to cause sensitization of TRPA1. By using site-directed mutagenesis, patch-clamp electrophysiology and calcium imaging we identify four amino acid residues, S86, S317, S428, and S972, as the principal targets of PKA-mediated phosphorylation and sensitization of TRPA1. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28076424/Sensitization_of_TRPA1_by_Protein_Kinase_A_ L2 - https://dx.plos.org/10.1371/journal.pone.0170097 DB - PRIME DP - Unbound Medicine ER -