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The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats.
J Appl Oral Sci. 2016 Nov-Dec; 24(6):597-606.JA

Abstract

Objectives

The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement.

Material and Methods

Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP.

Results

Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group.

Conclusions

These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.

Authors+Show Affiliations

Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28076465

Citation

Zhou, Yang, et al. "The Effect of Capsaicin On Expression Patterns of CGRP in Trigeminal Ganglion and Trigeminal Nucleus Caudalis Following Experimental Tooth Movement in Rats." Journal of Applied Oral Science : Revista FOB, vol. 24, no. 6, 2016, pp. 597-606.
Zhou Y, Long H, Ye N, et al. The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats. J Appl Oral Sci. 2016;24(6):597-606.
Zhou, Y., Long, H., Ye, N., Liao, L., Yang, X., Jian, F., Wang, Y., & Lai, W. (2016). The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats. Journal of Applied Oral Science : Revista FOB, 24(6), 597-606. https://doi.org/10.1590/1678-775720160150
Zhou Y, et al. The Effect of Capsaicin On Expression Patterns of CGRP in Trigeminal Ganglion and Trigeminal Nucleus Caudalis Following Experimental Tooth Movement in Rats. J Appl Oral Sci. 2016 Nov-Dec;24(6):597-606. PubMed PMID: 28076465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats. AU - Zhou,Yang, AU - Long,Hu, AU - Ye,Niansong, AU - Liao,Lina, AU - Yang,Xin, AU - Jian,Fan, AU - Wang,Yan, AU - Lai,Wenli, PY - 2016/04/04/received PY - 2016/08/14/accepted PY - 2017/1/12/entrez PY - 2017/1/12/pubmed PY - 2017/3/14/medline SP - 597 EP - 606 JF - Journal of applied oral science : revista FOB JO - J Appl Oral Sci VL - 24 IS - 6 N2 - Objectives: The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement. Material and Methods: Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP. Results: Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group. Conclusions: These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats. SN - 1678-7765 UR - https://www.unboundmedicine.com/medline/citation/28076465/The_effect_of_capsaicin_on_expression_patterns_of_CGRP_in_trigeminal_ganglion_and_trigeminal_nucleus_caudalis_following_experimental_tooth_movement_in_rats_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600597&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -