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Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy.
Biochem Biophys Res Commun. 2017 02 05; 483(2):885-891.BB

Abstract

The effects and mechanism of berberine (BBR) on hepatic injury after orthotopic liver transplantation (OLT) have not been well characterized. We examined the role of Sirt1/FoxO3α axis in the protective effect of BBR on ischemia/reperfusion injury after OLT. Adult male Wistar rats were randomly allocated into four groups: Sham, OLT, OLT with BBR pretreatment (BBR), OLT with BBR and Sirt1 inhibitor (EX527) pretreatment group (EX527). The liver function and oxidative stress level were measured by biochemical and histopathologic examinations. The formation of autophagosome was observed by transmission electron microscopy. The apoptotic rate was determined by TUNEL analysis and the apoptotic mRNA expression. The expression of Sirt1, FoxO3α, Beclin-1, LC3-II/LC3-I, p62 and the acetylation of FoxO3α were assayed by western blot assay and immunoprecipitation. Compared with the OLT group, BBR dramatically attenuated the histopathologic damage, restored the liver function, and decreased the oxidative stress level. Simultaneously, BBR significantly ameliorated apoptosis by decreasing the apoptotic rate and the expression of apoptotic mRNA in rats subjected to OLT. The level of Beclin-1 and LC3-II/LC3-I were upregulated with the inhibition of p62. The deacetylation of FoxO3α by Sirt1 was enhanced in the nuclear of liver after pretreated with BBR. However, the inhibition of Sirt1 by EX527 counteracted the protective effects of BBR. Thus, BBR preconditioning promotes liver transplant ischemia/reperfusion injury partly via activating Sirt1/FoxO3α mediated autophagy.

Authors+Show Affiliations

The Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Tianjin 300052, PR China.The Department of Anesthesiology, Tianjin First Center Hospital, 24 Fukang Road, Tianjin 300192, PR China.The Department of Anesthesiology, Tianjin First Center Hospital, 24 Fukang Road, Tianjin 300192, PR China.The Department of Anesthesiology, Tianjin First Center Hospital, 24 Fukang Road, Tianjin 300192, PR China.The Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Tianjin 300052, PR China.The Department of Anesthesiology, Tianjin First Center Hospital, 24 Fukang Road, Tianjin 300192, PR China.The Department of Anesthesiology, Tianjin First Center Hospital, 24 Fukang Road, Tianjin 300192, PR China. Electronic address: yuwenli999@sina.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28077277

Citation

Lin, Yuanbang, et al. "Berberine Protects Against Ischemia/reperfusion Injury After Orthotopic Liver Transplantation Via Activating Sirt1/FoxO3α Induced Autophagy." Biochemical and Biophysical Research Communications, vol. 483, no. 2, 2017, pp. 885-891.
Lin Y, Sheng M, Weng Y, et al. Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy. Biochem Biophys Res Commun. 2017;483(2):885-891.
Lin, Y., Sheng, M., Weng, Y., Xu, R., Lu, N., Du, H., & Yu, W. (2017). Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy. Biochemical and Biophysical Research Communications, 483(2), 885-891. https://doi.org/10.1016/j.bbrc.2017.01.028
Lin Y, et al. Berberine Protects Against Ischemia/reperfusion Injury After Orthotopic Liver Transplantation Via Activating Sirt1/FoxO3α Induced Autophagy. Biochem Biophys Res Commun. 2017 02 5;483(2):885-891. PubMed PMID: 28077277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy. AU - Lin,Yuanbang, AU - Sheng,Mingwei, AU - Weng,Yiqi, AU - Xu,Rubin, AU - Lu,Ning, AU - Du,Hongyin, AU - Yu,Wenli, Y1 - 2017/01/08/ PY - 2016/12/18/received PY - 2017/01/07/accepted PY - 2017/1/13/pubmed PY - 2017/6/6/medline PY - 2017/1/13/entrez KW - Apoptosis KW - Autophagy KW - Berberine KW - Liver transplantation KW - Sirtuin 1 SP - 885 EP - 891 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 483 IS - 2 N2 - The effects and mechanism of berberine (BBR) on hepatic injury after orthotopic liver transplantation (OLT) have not been well characterized. We examined the role of Sirt1/FoxO3α axis in the protective effect of BBR on ischemia/reperfusion injury after OLT. Adult male Wistar rats were randomly allocated into four groups: Sham, OLT, OLT with BBR pretreatment (BBR), OLT with BBR and Sirt1 inhibitor (EX527) pretreatment group (EX527). The liver function and oxidative stress level were measured by biochemical and histopathologic examinations. The formation of autophagosome was observed by transmission electron microscopy. The apoptotic rate was determined by TUNEL analysis and the apoptotic mRNA expression. The expression of Sirt1, FoxO3α, Beclin-1, LC3-II/LC3-I, p62 and the acetylation of FoxO3α were assayed by western blot assay and immunoprecipitation. Compared with the OLT group, BBR dramatically attenuated the histopathologic damage, restored the liver function, and decreased the oxidative stress level. Simultaneously, BBR significantly ameliorated apoptosis by decreasing the apoptotic rate and the expression of apoptotic mRNA in rats subjected to OLT. The level of Beclin-1 and LC3-II/LC3-I were upregulated with the inhibition of p62. The deacetylation of FoxO3α by Sirt1 was enhanced in the nuclear of liver after pretreated with BBR. However, the inhibition of Sirt1 by EX527 counteracted the protective effects of BBR. Thus, BBR preconditioning promotes liver transplant ischemia/reperfusion injury partly via activating Sirt1/FoxO3α mediated autophagy. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/28077277/Berberine_protects_against_ischemia/reperfusion_injury_after_orthotopic_liver_transplantation_via_activating_Sirt1/FoxO3α_induced_autophagy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(17)30037-2 DB - PRIME DP - Unbound Medicine ER -