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Effects of direct current electric fields on lung cancer cell electrotaxis in a PMMA-based microfluidic device.
Anal Bioanal Chem. 2017 Mar; 409(8):2163-2178.AB

Abstract

Tumor metastasis is the primary cause of cancer death. Numerous studies have demonstrated the electrotactic responses of various cancer cell types, and suggested its potential implications in metastasis. In this study, we used a microfluidic device to emulate endogenous direct current electric field (dcEF) environment, and studied the electrotactic migration of non-small cell lung cancer cell lines (H460, HCC827, H1299, and H1975) and the underlying mechanisms. These cell lines exhibited greatly different response in applied dcEFs (2-6 V/cm). While H460 cells (large cell carcinoma) showed slight migration toward cathode, H1299 cells (large cell carcinoma) showed increased motility and dcEF-dependent anodal migration with cell reorientation. H1975 cells (adenocarcinoma) showed dcEF-dependent cathodal migration with increased motility, and HCC827 cells (adenocarcinoma) responded positively in migration speed and reorientation but minimally in migrating directions to dcEF. Activation of MAPK and PI3K signaling pathways was found to be associated with the realignment and directed migration of lung cancer cells. In addition, both Ca2+ influx through activated stretch-activated calcium channels (SACCs) (but not voltage-gated calcium channels, VGCCs) and Ca2+ release from intracellular storage were involved in lung cancer cell electrotactic responses. The results demonstrated that the microfluidic device provided a stable and controllable microenvironment for cell electrotaxis study, and revealed that the electrotactic responses of lung cancer cells were heterogeneous and cell-type dependent, and multiple signals contributed to lung cancer cells electrotaxis.

Authors+Show Affiliations

Department of Biomedical Sciences, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong, Hong Kong, 999077, China. Key Laboratory of Biochip Technology, Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, No.8, Yuexing 1st Road, Nanshan District, Shenzhen, Guangdong, 518057, China.Department of Biomedical Sciences, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong, Hong Kong, 999077, China. Jinzhou Medical University, No.40, Section 3, Songpo Road, Linghe District, Jinzhou, Liaoning, 121000, China.Key Laboratory of Biochip Technology, Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, No.8, Yuexing 1st Road, Nanshan District, Shenzhen, Guangdong, 518057, China.Department of Developmental & Cell Biology, University of California, Irvine, CA, 92697, USA.Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, 999077, China.Department of Biomedical Sciences, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong, Hong Kong, 999077, China. bhmyang@cityu.edu.hk. Key Laboratory of Biochip Technology, Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, No.8, Yuexing 1st Road, Nanshan District, Shenzhen, Guangdong, 518057, China. bhmyang@cityu.edu.hk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28078410

Citation

Li, Yaping, et al. "Effects of Direct Current Electric Fields On Lung Cancer Cell Electrotaxis in a PMMA-based Microfluidic Device." Analytical and Bioanalytical Chemistry, vol. 409, no. 8, 2017, pp. 2163-2178.
Li Y, Xu T, Chen X, et al. Effects of direct current electric fields on lung cancer cell electrotaxis in a PMMA-based microfluidic device. Anal Bioanal Chem. 2017;409(8):2163-2178.
Li, Y., Xu, T., Chen, X., Lin, S., Cho, M., Sun, D., & Yang, M. (2017). Effects of direct current electric fields on lung cancer cell electrotaxis in a PMMA-based microfluidic device. Analytical and Bioanalytical Chemistry, 409(8), 2163-2178. https://doi.org/10.1007/s00216-016-0162-0
Li Y, et al. Effects of Direct Current Electric Fields On Lung Cancer Cell Electrotaxis in a PMMA-based Microfluidic Device. Anal Bioanal Chem. 2017;409(8):2163-2178. PubMed PMID: 28078410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of direct current electric fields on lung cancer cell electrotaxis in a PMMA-based microfluidic device. AU - Li,Yaping, AU - Xu,Tao, AU - Chen,Xiaomei, AU - Lin,Shin, AU - Cho,Michael, AU - Sun,Dong, AU - Yang,Mengsu, Y1 - 2017/01/11/ PY - 2016/08/21/received PY - 2016/12/16/accepted PY - 2016/12/14/revised PY - 2017/1/13/pubmed PY - 2018/3/10/medline PY - 2017/1/13/entrez KW - Electric field KW - Lung cancer KW - Microscale device KW - Migration SP - 2163 EP - 2178 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 409 IS - 8 N2 - Tumor metastasis is the primary cause of cancer death. Numerous studies have demonstrated the electrotactic responses of various cancer cell types, and suggested its potential implications in metastasis. In this study, we used a microfluidic device to emulate endogenous direct current electric field (dcEF) environment, and studied the electrotactic migration of non-small cell lung cancer cell lines (H460, HCC827, H1299, and H1975) and the underlying mechanisms. These cell lines exhibited greatly different response in applied dcEFs (2-6 V/cm). While H460 cells (large cell carcinoma) showed slight migration toward cathode, H1299 cells (large cell carcinoma) showed increased motility and dcEF-dependent anodal migration with cell reorientation. H1975 cells (adenocarcinoma) showed dcEF-dependent cathodal migration with increased motility, and HCC827 cells (adenocarcinoma) responded positively in migration speed and reorientation but minimally in migrating directions to dcEF. Activation of MAPK and PI3K signaling pathways was found to be associated with the realignment and directed migration of lung cancer cells. In addition, both Ca2+ influx through activated stretch-activated calcium channels (SACCs) (but not voltage-gated calcium channels, VGCCs) and Ca2+ release from intracellular storage were involved in lung cancer cell electrotactic responses. The results demonstrated that the microfluidic device provided a stable and controllable microenvironment for cell electrotaxis study, and revealed that the electrotactic responses of lung cancer cells were heterogeneous and cell-type dependent, and multiple signals contributed to lung cancer cells electrotaxis. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/28078410/Effects_of_direct_current_electric_fields_on_lung_cancer_cell_electrotaxis_in_a_PMMA_based_microfluidic_device_ L2 - https://dx.doi.org/10.1007/s00216-016-0162-0 DB - PRIME DP - Unbound Medicine ER -