Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation.
In Vitro Cell Dev Biol Anim. 2017 May; 53(5):448-457.VC

Abstract

Our purpose was to evaluate the protective effect of three marine omega-3 sources, fish oil (FO), krill oil (KO), and green-lipped mussel (GLM) against cartilage degradation. Canine cartilage explants were stimulated with either 10 ng/mL interleukin-1β (IL-1β) or IL-1β/oncostatin M (10 ng/mL each) and then treated with various concentrations of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA; 3 and 30 μg/mL), FO, KO, or GLM (250, 500, and 1000 μg/mL) for 28 days. Gene expression was then investigated in primary canine chondrocytes. Our results showed that DHA and EPA as well as omega-3 sources could suppress matrix degradation in cytokine-induced cartilage explants by significantly reducing the increase of sulfated glycosaminoglycans (s-GAGs) and preserving uronic acid and hydroxyproline content (except GLM). These agents were not able to reduce IL-1β-induced IL1B and TNFA expression but were able to down-regulate the expression of the catabolic genes MMP1, MMP3, and MMP13 and up-regulate the anabolic genes AGG and COL2A1; FO and KO were especially effective. Our findings indicated that FO and KO were superior to GLM for their protective effect against proteoglycan and collagen degradation. Hence, FO and KO could serve as promising sources of chondroprotective agents.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Buddhachat K

Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand. Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.

Siengdee P

Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.

Chomdej S

Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.

Soontornvipart K

Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

Nganvongpanit K

MeSH

AnimalsBivalviaCartilage, ArticularDocosahexaenoic AcidsDogsEicosapentaenoic AcidEuphausiaceaFatty Acids, Omega-3Fish OilsGene Expression RegulationGlycosaminoglycansInterleukin-1betaPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28078500

Citation

Buddhachat, Kittisak, et al. "Effects of Different Omega-3 Sources, Fish Oil, Krill Oil, and Green-lipped Mussel Against Cytokine-mediated Canine Cartilage Degradation." In Vitro Cellular & Developmental Biology. Animal, vol. 53, no. 5, 2017, pp. 448-457.

Buddhachat K, Siengdee P, Chomdej S, et al. Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation. In Vitro Cell Dev Biol Anim. 2017;53(5):448-457.

Buddhachat, K., Siengdee, P., Chomdej, S., Soontornvipart, K., & Nganvongpanit, K. (2017). Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation. In Vitro Cellular & Developmental Biology. Animal, 53(5), 448-457. https://doi.org/10.1007/s11626-016-0125-y

Buddhachat K, et al. Effects of Different Omega-3 Sources, Fish Oil, Krill Oil, and Green-lipped Mussel Against Cytokine-mediated Canine Cartilage Degradation. In Vitro Cell Dev Biol Anim. 2017;53(5):448-457. PubMed PMID: 28078500.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation. AU - Buddhachat,Kittisak, AU - Siengdee,Puntita, AU - Chomdej,Siriwadee, AU - Soontornvipart,Kumpanart, AU - Nganvongpanit,Korakot, Y1 - 2017/01/11/ PY - 2016/07/27/received PY - 2016/12/20/accepted PY - 2017/1/13/pubmed PY - 2018/3/13/medline PY - 2017/1/13/entrez KW - Cartilage KW - Docosahexaenoic acid KW - Eicosapentaenoic acid KW - Omega-3 KW - Osteoarthritis SP - 448 EP - 457 JF - In vitro cellular & developmental biology. Animal JO - In Vitro Cell Dev Biol Anim VL - 53 IS - 5 N2 - Our purpose was to evaluate the protective effect of three marine omega-3 sources, fish oil (FO), krill oil (KO), and green-lipped mussel (GLM) against cartilage degradation. Canine cartilage explants were stimulated with either 10 ng/mL interleukin-1β (IL-1β) or IL-1β/oncostatin M (10 ng/mL each) and then treated with various concentrations of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA; 3 and 30 μg/mL), FO, KO, or GLM (250, 500, and 1000 μg/mL) for 28 days. Gene expression was then investigated in primary canine chondrocytes. Our results showed that DHA and EPA as well as omega-3 sources could suppress matrix degradation in cytokine-induced cartilage explants by significantly reducing the increase of sulfated glycosaminoglycans (s-GAGs) and preserving uronic acid and hydroxyproline content (except GLM). These agents were not able to reduce IL-1β-induced IL1B and TNFA expression but were able to down-regulate the expression of the catabolic genes MMP1, MMP3, and MMP13 and up-regulate the anabolic genes AGG and COL2A1; FO and KO were especially effective. Our findings indicated that FO and KO were superior to GLM for their protective effect against proteoglycan and collagen degradation. Hence, FO and KO could serve as promising sources of chondroprotective agents. SN - 1543-706X UR - https://www.unboundmedicine.com/medline/citation/28078500/Effects_of_different_omega_3_sources_fish_oil_krill_oil_and_green_lipped_mussel_against_cytokine_mediated_canine_cartilage_degradation_ DB - PRIME DP - Unbound Medicine ER -

Tags

Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation.In Vitro Cell Dev Biol Anim. 2017 May; 53(5):448-457.VC