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Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells-like modulated by Toll-like receptors 2 and 7/8 in adults with atopic dermatitis.
Int J Dermatol. 2017 Jun; 56(6):630-635.IJ

Abstract

BACKGROUD

Atopic dermatitis (AD) is a chronic, inflammatory skin disease characterized by intense pruritus and xerosis. Dendritic cells (DC) play an essential role in tissue inflammation in atopic dermatitis (AD) skin, especially the inflammatory epidermal dendritic cells (IDEC), a particular subset of myeloid dendritic cells (mDC). The aim of the present study was to assess the phenotype and function of mDC and circulating IDEC-like in peripheral blood mononuclear cells (PBMC) of adults with AD.

METHODS

We selected 21 AD patients and 21 non-AD controls, age and gender matched. Expressions of FcεRI, CD36, TNF, IFN- γ, and IL-10 in mDC were analyzed by flow cytometry under various stimuli, such as staphylococcal enterotoxin B (SEB), TLR2 (Pam3CSK4), TLR4 (LPS), and TLR7/8 (CL097) agonists.

RESULTS

The most prominent findings in AD patients were: (i) enhanced frequency of IL-10 under TLR4 (LPS), and decreased frequency of IFN-γ and TNF under TLR2 (Pam3CSK4) and 7/8 (CL097) stimulation in classic mDC; (ii) elevation of circulating IDEC-like frequency with TLR2 (Pam3CSK4) stimuli, augmented frequency of IFN-γ in nonstimulated condition, and of IL-10 under TLR7/8 (CL097) stimuli in IDEC-like population.

CONCLUSIONS

In AD individuals, classic mDC showed an immunomodulatory profile, favoring tolerance in a combined action with IDEC-like, and inducing Th1 polarization. Our findings indicate a potential role of IDEC-like in the maintenance of inflammation in atopic dermatitis patients; moreover, IDEC-like may exert a regulatory impact on T cells of AD individuals through IL-10, often induced by agonist mimicking single stranded RNA virus.

Authors+Show Affiliations

Department of Dermatology, Laboratory of Dermatology and Immunodeficiency (LIM-56), University of Sao Paulo School of Medicine, Sao Paulo-SP, Brazil.Department of Dermatology, Laboratory of Dermatology and Immunodeficiency (LIM-56), University of Sao Paulo School of Medicine, Sao Paulo-SP, Brazil.Department of Dermatology, Laboratory of Dermatology and Immunodeficiency (LIM-56), University of Sao Paulo School of Medicine, Sao Paulo-SP, Brazil.Department of Dermatology, Laboratory of Dermatology and Immunodeficiency (LIM-56), University of Sao Paulo School of Medicine, Sao Paulo-SP, Brazil.Department of Dermatology, Laboratory of Dermatology and Immunodeficiency (LIM-56), University of Sao Paulo School of Medicine, Sao Paulo-SP, Brazil.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28083892

Citation

Dos Santos, Vanessa G., et al. "Evidence of Regulatory Myeloid Dendritic Cells and Circulating Inflammatory Epidermal Dendritic Cells-like Modulated By Toll-like Receptors 2 and 7/8 in Adults With Atopic Dermatitis." International Journal of Dermatology, vol. 56, no. 6, 2017, pp. 630-635.
Dos Santos VG, Orfali RL, de Oliveira Titz T, et al. Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells-like modulated by Toll-like receptors 2 and 7/8 in adults with atopic dermatitis. Int J Dermatol. 2017;56(6):630-635.
Dos Santos, V. G., Orfali, R. L., de Oliveira Titz, T., da Silva Duarte, A. J., Sato, M. N., & Aoki, V. (2017). Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells-like modulated by Toll-like receptors 2 and 7/8 in adults with atopic dermatitis. International Journal of Dermatology, 56(6), 630-635. https://doi.org/10.1111/ijd.13537
Dos Santos VG, et al. Evidence of Regulatory Myeloid Dendritic Cells and Circulating Inflammatory Epidermal Dendritic Cells-like Modulated By Toll-like Receptors 2 and 7/8 in Adults With Atopic Dermatitis. Int J Dermatol. 2017;56(6):630-635. PubMed PMID: 28083892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells-like modulated by Toll-like receptors 2 and 7/8 in adults with atopic dermatitis. AU - Dos Santos,Vanessa G, AU - Orfali,Raquel L, AU - de Oliveira Titz,Tiago, AU - da Silva Duarte,Alberto J, AU - Sato,Maria N, AU - Aoki,Valeria, Y1 - 2017/01/12/ PY - 2016/07/25/received PY - 2016/09/19/revised PY - 2016/11/14/accepted PY - 2017/1/14/pubmed PY - 2018/3/14/medline PY - 2017/1/14/entrez SP - 630 EP - 635 JF - International journal of dermatology JO - Int. J. Dermatol. VL - 56 IS - 6 N2 - BACKGROUD: Atopic dermatitis (AD) is a chronic, inflammatory skin disease characterized by intense pruritus and xerosis. Dendritic cells (DC) play an essential role in tissue inflammation in atopic dermatitis (AD) skin, especially the inflammatory epidermal dendritic cells (IDEC), a particular subset of myeloid dendritic cells (mDC). The aim of the present study was to assess the phenotype and function of mDC and circulating IDEC-like in peripheral blood mononuclear cells (PBMC) of adults with AD. METHODS: We selected 21 AD patients and 21 non-AD controls, age and gender matched. Expressions of FcεRI, CD36, TNF, IFN- γ, and IL-10 in mDC were analyzed by flow cytometry under various stimuli, such as staphylococcal enterotoxin B (SEB), TLR2 (Pam3CSK4), TLR4 (LPS), and TLR7/8 (CL097) agonists. RESULTS: The most prominent findings in AD patients were: (i) enhanced frequency of IL-10 under TLR4 (LPS), and decreased frequency of IFN-γ and TNF under TLR2 (Pam3CSK4) and 7/8 (CL097) stimulation in classic mDC; (ii) elevation of circulating IDEC-like frequency with TLR2 (Pam3CSK4) stimuli, augmented frequency of IFN-γ in nonstimulated condition, and of IL-10 under TLR7/8 (CL097) stimuli in IDEC-like population. CONCLUSIONS: In AD individuals, classic mDC showed an immunomodulatory profile, favoring tolerance in a combined action with IDEC-like, and inducing Th1 polarization. Our findings indicate a potential role of IDEC-like in the maintenance of inflammation in atopic dermatitis patients; moreover, IDEC-like may exert a regulatory impact on T cells of AD individuals through IL-10, often induced by agonist mimicking single stranded RNA virus. SN - 1365-4632 UR - https://www.unboundmedicine.com/medline/citation/28083892/Evidence_of_regulatory_myeloid_dendritic_cells_and_circulating_inflammatory_epidermal_dendritic_cells_like_modulated_by_Toll_like_receptors_2_and_7/8_in_adults_with_atopic_dermatitis_ L2 - https://doi.org/10.1111/ijd.13537 DB - PRIME DP - Unbound Medicine ER -