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Moderate physical activity promotes basal hepatic autophagy in diet-induced obese mice.
Appl Physiol Nutr Metab 2017; 42(2):148-156AP

Abstract

Obesity is a known risk factor for the development of hepatic disease; obesity-induced fatty liver can lead to inflammation, steatosis, and cirrhosis and is associated with degeneration of the mitochondria. Lifestyle interventions such as physical activity may ameliorate this condition. The purpose of this study was to investigate regulation of mitochondrial and autophagy quality control in liver following Western diet-induced obesity and voluntary physical activity. Eight-week-old C57BL/6J mice were fed a Western diet (WD) or normal chow (NC, control) for 4 weeks; afterwards, groups were divided into voluntary wheel running (VWR) or sedentary (SED) conditions for an additional 4 weeks. WD-SED animals had a median histology score of 2, whereas WD-VWR was not different from NC groups (median score 1). There was no difference in mRNA of inflammatory markers Il6 and Tnfa in WD animals. WD animals had 50% lower mitochondrial content (COX IV and Cytochrome C proteins), 50% lower Pgc1a mRNA content, and reduced content of mitochondrial fusion and fission markers. Markers of autophagy were increased in VWR animals, regardless of obesity, as measured by 50% greater LC3-II/I ratio and 40% lower p62 protein content. BNIP3 protein content was 30% less in WD animals compared with NC animals, regardless of physical activity. Diet-induced obesity results in derangements in mitochondrial quality control that appear to occur prior to the onset of hepatic inflammation. Moderate physical activity appears to enhance basal autophagy in the liver; increased autophagy may provide protection from hepatic fat accumulation.

Authors+Show Affiliations

a Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.a Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.a Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.b Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.b Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.c Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.d California Animal Health and Food Safety Laboratory, University of California-Davis School of Veterinary Medicine, Davis, CA 95616, USA.b Exercise Muscle Biology Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.a Integrative Muscle Metabolism Laboratory, Human Performance Laboratory, Department of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR 72701, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28084795

Citation

Rosa-Caldwell, Megan E., et al. "Moderate Physical Activity Promotes Basal Hepatic Autophagy in Diet-induced Obese Mice." Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, vol. 42, no. 2, 2017, pp. 148-156.
Rosa-Caldwell ME, Lee DE, Brown JL, et al. Moderate physical activity promotes basal hepatic autophagy in diet-induced obese mice. Appl Physiol Nutr Metab. 2017;42(2):148-156.
Rosa-Caldwell, M. E., Lee, D. E., Brown, J. L., Brown, L. A., Perry, R. A., Greene, E. S., ... Greene, N. P. (2017). Moderate physical activity promotes basal hepatic autophagy in diet-induced obese mice. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, 42(2), pp. 148-156. doi:10.1139/apnm-2016-0280.
Rosa-Caldwell ME, et al. Moderate Physical Activity Promotes Basal Hepatic Autophagy in Diet-induced Obese Mice. Appl Physiol Nutr Metab. 2017;42(2):148-156. PubMed PMID: 28084795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moderate physical activity promotes basal hepatic autophagy in diet-induced obese mice. AU - Rosa-Caldwell,Megan E, AU - Lee,David E, AU - Brown,Jacob L, AU - Brown,Lemuel A, AU - Perry,Richard A,Jr AU - Greene,Elizabeth S, AU - Carvallo Chaigneau,Francisco R, AU - Washington,Tyrone A, AU - Greene,Nicholas P, Y1 - 2016/10/12/ PY - 2017/1/14/pubmed PY - 2017/2/6/medline PY - 2017/1/14/entrez KW - diabetes KW - diabète KW - exercice physique KW - exercise KW - insulin resistance KW - insulinorésistance KW - mitochondria KW - mitochondries KW - nonalcoholic fatty liver disease KW - stéatose hépatique non alcoolique SP - 148 EP - 156 JF - Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme JO - Appl Physiol Nutr Metab VL - 42 IS - 2 N2 - Obesity is a known risk factor for the development of hepatic disease; obesity-induced fatty liver can lead to inflammation, steatosis, and cirrhosis and is associated with degeneration of the mitochondria. Lifestyle interventions such as physical activity may ameliorate this condition. The purpose of this study was to investigate regulation of mitochondrial and autophagy quality control in liver following Western diet-induced obesity and voluntary physical activity. Eight-week-old C57BL/6J mice were fed a Western diet (WD) or normal chow (NC, control) for 4 weeks; afterwards, groups were divided into voluntary wheel running (VWR) or sedentary (SED) conditions for an additional 4 weeks. WD-SED animals had a median histology score of 2, whereas WD-VWR was not different from NC groups (median score 1). There was no difference in mRNA of inflammatory markers Il6 and Tnfa in WD animals. WD animals had 50% lower mitochondrial content (COX IV and Cytochrome C proteins), 50% lower Pgc1a mRNA content, and reduced content of mitochondrial fusion and fission markers. Markers of autophagy were increased in VWR animals, regardless of obesity, as measured by 50% greater LC3-II/I ratio and 40% lower p62 protein content. BNIP3 protein content was 30% less in WD animals compared with NC animals, regardless of physical activity. Diet-induced obesity results in derangements in mitochondrial quality control that appear to occur prior to the onset of hepatic inflammation. Moderate physical activity appears to enhance basal autophagy in the liver; increased autophagy may provide protection from hepatic fat accumulation. SN - 1715-5320 UR - https://www.unboundmedicine.com/medline/citation/28084795/Moderate_physical_activity_promotes_basal_hepatic_autophagy_in_diet_induced_obese_mice_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/apnm-2016-0280?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -