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Possible role of prostaglandins as negative regulators in growth stimulation by tumor necrosis factor and epidermal growth factor in human fibroblasts.
J Cell Physiol. 1989 Nov; 141(2):275-80.JC

Abstract

Recombinant tumor necrosis factor (TNF), epidermal growth factor (EGF), and transforming growth factor beta (TGF-beta) stimulated growth of confluent human diploid fibroblasts (FS-4 cells) in the presence of fetal calf serum. TGF-beta synergistically enhanced both the TNF- and EGF-stimulated cell growth, whereas synergism between the mitogenic action of EGF and that of TNF was not observed. When indomethacin or acetylsalicylic acid, an inhibitor of prostaglandin production, was added to FS-4 cells, cell growth stimulated by EGF or TNF was increased, suggesting that prostaglandins induced by these mitogens antagonize their growth stimulatory actions. In contrast, neither indomethacin nor acetylsalicylic acid had a significant effect on the TGF-beta-induced growth of FS-4 cells. Mitogenic responses of indomethacin-treated cells to EGF, TNF, and TGF-beta were similarly suppressed by the addition of exogenous prostaglandin D2 (PGD2). Other prostaglandins such as PGE2 and PGF2 produced less inhibition of the cell growth.

Authors+Show Affiliations

Hori T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Kashiyama S
No affiliation info available
Hayakawa M
No affiliation info available
Shibamoto S
No affiliation info available
Tsujimoto M
No affiliation info available
Oku N
No affiliation info available
Ito F
No affiliation info available

MeSH

Arachidonic AcidsAspirinCell DivisionCell LineCells, CulturedChild, PreschoolEpidermal Growth FactorFibroblastsHumansIndomethacinProstaglandinsTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2808538

Citation

Hori, T, et al. "Possible Role of Prostaglandins as Negative Regulators in Growth Stimulation By Tumor Necrosis Factor and Epidermal Growth Factor in Human Fibroblasts." Journal of Cellular Physiology, vol. 141, no. 2, 1989, pp. 275-80.
Hori T, Kashiyama S, Hayakawa M, et al. Possible role of prostaglandins as negative regulators in growth stimulation by tumor necrosis factor and epidermal growth factor in human fibroblasts. J Cell Physiol. 1989;141(2):275-80.
Hori, T., Kashiyama, S., Hayakawa, M., Shibamoto, S., Tsujimoto, M., Oku, N., & Ito, F. (1989). Possible role of prostaglandins as negative regulators in growth stimulation by tumor necrosis factor and epidermal growth factor in human fibroblasts. Journal of Cellular Physiology, 141(2), 275-80.
Hori T, et al. Possible Role of Prostaglandins as Negative Regulators in Growth Stimulation By Tumor Necrosis Factor and Epidermal Growth Factor in Human Fibroblasts. J Cell Physiol. 1989;141(2):275-80. PubMed PMID: 2808538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible role of prostaglandins as negative regulators in growth stimulation by tumor necrosis factor and epidermal growth factor in human fibroblasts. AU - Hori,T, AU - Kashiyama,S, AU - Hayakawa,M, AU - Shibamoto,S, AU - Tsujimoto,M, AU - Oku,N, AU - Ito,F, PY - 1989/11/1/pubmed PY - 1989/11/1/medline PY - 1989/11/1/entrez SP - 275 EP - 80 JF - Journal of cellular physiology JO - J Cell Physiol VL - 141 IS - 2 N2 - Recombinant tumor necrosis factor (TNF), epidermal growth factor (EGF), and transforming growth factor beta (TGF-beta) stimulated growth of confluent human diploid fibroblasts (FS-4 cells) in the presence of fetal calf serum. TGF-beta synergistically enhanced both the TNF- and EGF-stimulated cell growth, whereas synergism between the mitogenic action of EGF and that of TNF was not observed. When indomethacin or acetylsalicylic acid, an inhibitor of prostaglandin production, was added to FS-4 cells, cell growth stimulated by EGF or TNF was increased, suggesting that prostaglandins induced by these mitogens antagonize their growth stimulatory actions. In contrast, neither indomethacin nor acetylsalicylic acid had a significant effect on the TGF-beta-induced growth of FS-4 cells. Mitogenic responses of indomethacin-treated cells to EGF, TNF, and TGF-beta were similarly suppressed by the addition of exogenous prostaglandin D2 (PGD2). Other prostaglandins such as PGE2 and PGF2 produced less inhibition of the cell growth. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/2808538/Possible_role_of_prostaglandins_as_negative_regulators_in_growth_stimulation_by_tumor_necrosis_factor_and_epidermal_growth_factor_in_human_fibroblasts_ DB - PRIME DP - Unbound Medicine ER -