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Transcriptional response of skeletal muscle to a low protein perinatal diet in rat offspring at different ages: The role of key enzymes of glucose-fatty acid oxidation.
J Nutr Biochem 2017; 41:117-123JN

Abstract

Skeletal muscle is a plastic tissue during development with distinctive acute and chronic response to maternal protein restriction. This study evaluated gene and protein expression of key-enzymes of glycolytic pathway (HK2, PFK, PDK4 and CS), and fatty acid oxidation (CPT1 and β-HAD) of two different types of skeletal muscle [soleus and extensor digitorium longus (EDL)] from offspring rats at 30 and 90 days of age, exposed to maternal isoenergetic low protein diet throughout gestation and lactation. Pups from dams fed 17% protein diet (n=5, normal protein, Np), and low protein pups from dams fed 8% casein diet (low protein, Lp, n=5) were evaluated. Offspring were sacrificed either 30 or 90 days old. Soleus and EDL were analyzed for mRNA and protein expression by quantitative PCR and western blotting, respectively. Soleus was more affected by Lp maternal diet at 90 days by down-regulation of key enzymes of glycolytic pathway, in particular HK2 and PDK4 with a concomitant reduction of β-HAD mRNA. For EDL, the effects of Lp maternal diet were more pronounced at 30 days, as the transcriptional key enzymes of glycolytic pathway were down-regulated. One important finding was that the observed acute (30 days) transcriptional changes did not remain in adult Lp rats (90 days), except for PDK4. The robust PDK4 mRNA down-regulation, observed in both soleus and EDL, and at both ages, and the consequent down-regulation of the PDK4 protein expression can be responsible for a state of reduced metabolic flexibility of skeletal muscle in response to maternal low protein diet.

Authors+Show Affiliations

Department of Nutrition, Federal University of Paraiba, Brazil; Carmen (Cardiology, Metabolism and Nutrition) Laboratory; INSERM U1060; Lyon-1 University, South Lyon Medical Faculty; 69921, Oullins, France.Department of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, - Pernambuco, 55608-680, Brazil.Department of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, - Pernambuco, 55608-680, Brazil.Carmen (Cardiology, Metabolism and Nutrition) Laboratory; INSERM U1060; Lyon-1 University, South Lyon Medical Faculty; 69921, Oullins, France.Department of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, - Pernambuco, 55608-680, Brazil. Electronic address: carolleandro22@gmail.com.Carmen (Cardiology, Metabolism and Nutrition) Laboratory; INSERM U1060; Lyon-1 University, South Lyon Medical Faculty; 69921, Oullins, France.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28088654

Citation

de Brito Alves, José Luiz, et al. "Transcriptional Response of Skeletal Muscle to a Low Protein Perinatal Diet in Rat Offspring at Different Ages: the Role of Key Enzymes of Glucose-fatty Acid Oxidation." The Journal of Nutritional Biochemistry, vol. 41, 2017, pp. 117-123.
de Brito Alves JL, Toscano AE, da Costa-Silva JH, et al. Transcriptional response of skeletal muscle to a low protein perinatal diet in rat offspring at different ages: The role of key enzymes of glucose-fatty acid oxidation. J Nutr Biochem. 2017;41:117-123.
de Brito Alves, J. L., Toscano, A. E., da Costa-Silva, J. H., Vidal, H., Leandro, C. G., & Pirola, L. (2017). Transcriptional response of skeletal muscle to a low protein perinatal diet in rat offspring at different ages: The role of key enzymes of glucose-fatty acid oxidation. The Journal of Nutritional Biochemistry, 41, pp. 117-123. doi:10.1016/j.jnutbio.2016.12.002.
de Brito Alves JL, et al. Transcriptional Response of Skeletal Muscle to a Low Protein Perinatal Diet in Rat Offspring at Different Ages: the Role of Key Enzymes of Glucose-fatty Acid Oxidation. J Nutr Biochem. 2017;41:117-123. PubMed PMID: 28088654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional response of skeletal muscle to a low protein perinatal diet in rat offspring at different ages: The role of key enzymes of glucose-fatty acid oxidation. AU - de Brito Alves,José Luiz, AU - Toscano,Ana Elisa, AU - da Costa-Silva,João Henrique, AU - Vidal,Hubert, AU - Leandro,Carol Góis, AU - Pirola,Luciano, Y1 - 2016/12/15/ PY - 2016/07/21/received PY - 2016/10/29/revised PY - 2016/12/09/accepted PY - 2017/1/16/pubmed PY - 2017/12/23/medline PY - 2017/1/16/entrez KW - Maternal undernutrition KW - Metabolic flexibility KW - Metabolism KW - Muscle KW - Phenotypic plasticity KW - Rats SP - 117 EP - 123 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 41 N2 - Skeletal muscle is a plastic tissue during development with distinctive acute and chronic response to maternal protein restriction. This study evaluated gene and protein expression of key-enzymes of glycolytic pathway (HK2, PFK, PDK4 and CS), and fatty acid oxidation (CPT1 and β-HAD) of two different types of skeletal muscle [soleus and extensor digitorium longus (EDL)] from offspring rats at 30 and 90 days of age, exposed to maternal isoenergetic low protein diet throughout gestation and lactation. Pups from dams fed 17% protein diet (n=5, normal protein, Np), and low protein pups from dams fed 8% casein diet (low protein, Lp, n=5) were evaluated. Offspring were sacrificed either 30 or 90 days old. Soleus and EDL were analyzed for mRNA and protein expression by quantitative PCR and western blotting, respectively. Soleus was more affected by Lp maternal diet at 90 days by down-regulation of key enzymes of glycolytic pathway, in particular HK2 and PDK4 with a concomitant reduction of β-HAD mRNA. For EDL, the effects of Lp maternal diet were more pronounced at 30 days, as the transcriptional key enzymes of glycolytic pathway were down-regulated. One important finding was that the observed acute (30 days) transcriptional changes did not remain in adult Lp rats (90 days), except for PDK4. The robust PDK4 mRNA down-regulation, observed in both soleus and EDL, and at both ages, and the consequent down-regulation of the PDK4 protein expression can be responsible for a state of reduced metabolic flexibility of skeletal muscle in response to maternal low protein diet. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/28088654/Transcriptional_response_of_skeletal_muscle_to_a_low_protein_perinatal_diet_in_rat_offspring_at_different_ages:_The_role_of_key_enzymes_of_glucose_fatty_acid_oxidation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(16)30272-8 DB - PRIME DP - Unbound Medicine ER -