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Quantitative Determination of Lactones in Piper methysticum (Kava-Kava) by Supercritical Fluid Chromatography.
Planta Med. 2017 Aug; 83(12-13):1053-1057.PM

Abstract

A fast and validated supercritical fluid chromatography method for the quantitative determination of major lactones in Piper methysticum, a plant used against nervous anxiety, stress, and restlessness, was developed. The baseline separation of dihydrokavain, demethoxyyangonin, kavain, yangonin, dihydromethysticin, and methysticin was possible in less than 4 min on an Aquity UPC2 BEH 1.7 µm column, in combination with a mobile phase comprising CO2 and methanol with diethylamine. The column temperature had a great impact on the results because only at 70 °C could kavain and yangonin be fully resolved. With correlation coefficients above 0.998, recovery rates between 95.9 and 104.1 % as well as limit of detection values below 1.5 ng on-column, the procedure fulfilled all validation requirements and was well suited for the quantitative analysis of commercial products containing P. methysticum root powder and/or extract. All of them contained the target analytes, however, the absolute content of lactones was quite variable. Accordingly, depending on the product, the total daily intake of lactones varied from 56 to 312 mg. Concerning speed, selectivity, and environmental friendly operation, this supercritical fluid chromatography approach surpasses all previously reported ones.

Authors+Show Affiliations

Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innsbruck, Austria.Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innsbruck, Austria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28095587

Citation

Murauer, Adele, and Markus Ganzera. "Quantitative Determination of Lactones in Piper Methysticum (Kava-Kava) By Supercritical Fluid Chromatography." Planta Medica, vol. 83, no. 12-13, 2017, pp. 1053-1057.
Murauer A, Ganzera M. Quantitative Determination of Lactones in Piper methysticum (Kava-Kava) by Supercritical Fluid Chromatography. Planta Med. 2017;83(12-13):1053-1057.
Murauer, A., & Ganzera, M. (2017). Quantitative Determination of Lactones in Piper methysticum (Kava-Kava) by Supercritical Fluid Chromatography. Planta Medica, 83(12-13), 1053-1057. https://doi.org/10.1055/s-0043-100632
Murauer A, Ganzera M. Quantitative Determination of Lactones in Piper Methysticum (Kava-Kava) By Supercritical Fluid Chromatography. Planta Med. 2017;83(12-13):1053-1057. PubMed PMID: 28095587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative Determination of Lactones in Piper methysticum (Kava-Kava) by Supercritical Fluid Chromatography. AU - Murauer,Adele, AU - Ganzera,Markus, Y1 - 2017/01/17/ PY - 2017/1/18/pubmed PY - 2018/1/13/medline PY - 2017/1/18/entrez SP - 1053 EP - 1057 JF - Planta medica JO - Planta Med VL - 83 IS - 12-13 N2 - A fast and validated supercritical fluid chromatography method for the quantitative determination of major lactones in Piper methysticum, a plant used against nervous anxiety, stress, and restlessness, was developed. The baseline separation of dihydrokavain, demethoxyyangonin, kavain, yangonin, dihydromethysticin, and methysticin was possible in less than 4 min on an Aquity UPC2 BEH 1.7 µm column, in combination with a mobile phase comprising CO2 and methanol with diethylamine. The column temperature had a great impact on the results because only at 70 °C could kavain and yangonin be fully resolved. With correlation coefficients above 0.998, recovery rates between 95.9 and 104.1 % as well as limit of detection values below 1.5 ng on-column, the procedure fulfilled all validation requirements and was well suited for the quantitative analysis of commercial products containing P. methysticum root powder and/or extract. All of them contained the target analytes, however, the absolute content of lactones was quite variable. Accordingly, depending on the product, the total daily intake of lactones varied from 56 to 312 mg. Concerning speed, selectivity, and environmental friendly operation, this supercritical fluid chromatography approach surpasses all previously reported ones. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/28095587/Quantitative_Determination_of_Lactones_in_Piper_methysticum__Kava_Kava__by_Supercritical_Fluid_Chromatography_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0043-100632 DB - PRIME DP - Unbound Medicine ER -