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Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity.
BMC Complement Altern Med. 2017 Jan 17; 17(1):52.BC

Abstract

BACKGROUND

Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders.

METHODS

The aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator; tumour necrosis factor (TNF)-α were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performed RESULTS: Fifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-α in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression.

CONCLUSION

These results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-α, might be the possible hepatoprotective mechanisms.

Authors+Show Affiliations

Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt. rehambkr@yahoo.com.Pharmacology Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.Pharmaceutics Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt. Axe of Regenerative Medicine, Faculty of Medicine, Universite Laval, Quebec City, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28095910

Citation

Bakr, Riham Omar, et al. "Profile of Bioactive Compounds in Nymphaea Alba L. Leaves Growing in Egypt: Hepatoprotective, Antioxidant and Anti-inflammatory Activity." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 52.
Bakr RO, El-Naa MM, Zaghloul SS, et al. Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity. BMC Complement Altern Med. 2017;17(1):52.
Bakr, R. O., El-Naa, M. M., Zaghloul, S. S., & Omar, M. M. (2017). Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity. BMC Complementary and Alternative Medicine, 17(1), 52. https://doi.org/10.1186/s12906-017-1561-2
Bakr RO, et al. Profile of Bioactive Compounds in Nymphaea Alba L. Leaves Growing in Egypt: Hepatoprotective, Antioxidant and Anti-inflammatory Activity. BMC Complement Altern Med. 2017 Jan 17;17(1):52. PubMed PMID: 28095910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity. AU - Bakr,Riham Omar, AU - El-Naa,Mona Mohamed, AU - Zaghloul,Soumaya Saad, AU - Omar,Mahmoud Mohamed, Y1 - 2017/01/17/ PY - 2016/10/12/received PY - 2017/01/05/accepted PY - 2017/1/19/entrez PY - 2017/1/18/pubmed PY - 2017/1/26/medline KW - Ellagitannins KW - Hepatotoxicity KW - Inflammation KW - Nymphaea alba KW - Oxidative stress KW - Palmitic acid SP - 52 EP - 52 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders. METHODS: The aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator; tumour necrosis factor (TNF)-α were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performed RESULTS: Fifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-α in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression. CONCLUSION: These results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-α, might be the possible hepatoprotective mechanisms. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28095910/Profile_of_bioactive_compounds_in_Nymphaea_alba_L__leaves_growing_in_Egypt:_hepatoprotective_antioxidant_and_anti_inflammatory_activity_ DB - PRIME DP - Unbound Medicine ER -