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Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton mentagrophytes Strains Isolated from Patients with Dermatophytosis.
Mycopathologia. 2017 Jun; 182(5-6):487-493.M

Abstract

According to epidemiological, clinical and mycological criteria, it has long been admitted that the Trichophyton mentagrophytes species includes two varieties: a zoophilic variety (var. mentagrophytes) and an anthropophilic variety (var. interdigitale) that involve the upper and the lower part of the body, respectively. The further application of molecular techniques to the characterization of dermatophyte strains showed that this classification is unreliable. The aim of our study was to assess the usefulness of PCR-RFLP (restriction fragment length polymorphism) and sequencing in the characterization of T. mentagrophytes strains taken from Tunisian patients. The study was carried out in 2008 in the laboratory of Parasitology-Mycology of Farhat Hached University Hospital, Sousse, Tunisia. A total of 133 strains were isolated from 133 patients addressed to the laboratory for dermatological lesions very evocative of dermatomycosis. Eighty strains were isolated from lesions located on the lower part of the body (onychomycosis, tinea pedis) and 53 strains from the upper part of the body (tinea capitis, tinea corporis). All strains were submitted to mycological examination (direct microscopic examination and culture on Sabouraud medium) and further investigated by using RFLP analysis of the PCR-amplified ITS1-5.8 s-ITS2 region of the ribosomal DNA and the MvaI restriction enzyme. In addition, 62 strains were further submitted to a sequencing of the ITS1-5.8 s-ITS2 region. On the basis of mycological criteria, all strains were diagnosed as T. mentagrophytes. All strains produced the same RFLP pattern and were identified as T. mentagrophytes interdigitale regardless of the location of lesions. Out of the 62 sequenced strains, 16 were found anthropophilic and 46 were zoophilic. In conclusion, all strains provisionally diagnosed as T. mentagrophytes on the basis of mycological criteria were shown to belong to T. interdigitale by using PCR-RFLP and sequencing irrespective of the site of lesions. The predominance of zoophilic strains needs further investigation.

Authors+Show Affiliations

Parasitology-Mycology Laboratory, Faculty of Medicine, Farhat Hached University Hospital, Mohamed El Karoui Street, 4002, Sousse, Tunisia. dhib.imen@yahoo.fr.Parasitology-Mycology Laboratory, Faculty of Medicine, Farhat Hached University Hospital, Mohamed El Karoui Street, 4002, Sousse, Tunisia.Parasitology-Mycology Laboratory, Faculty of Medicine, Farhat Hached University Hospital, Mohamed El Karoui Street, 4002, Sousse, Tunisia.Immunology and Genetic Laboratory, Faculty of Medicine, Sousse, Tunisia.Parasitology-Mycology Laboratory, Faculty of Medicine, Farhat Hached University Hospital, Mohamed El Karoui Street, 4002, Sousse, Tunisia.Immunology and Genetic Laboratory, Faculty of Medicine, Sousse, Tunisia.Parasitology-Mycology Laboratory, Faculty of Medicine, Farhat Hached University Hospital, Mohamed El Karoui Street, 4002, Sousse, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28101694

Citation

Dhib, I, et al. "Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton Mentagrophytes Strains Isolated From Patients With Dermatophytosis." Mycopathologia, vol. 182, no. 5-6, 2017, pp. 487-493.
Dhib I, Khammari I, Yaacoub A, et al. Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton mentagrophytes Strains Isolated from Patients with Dermatophytosis. Mycopathologia. 2017;182(5-6):487-493.
Dhib, I., Khammari, I., Yaacoub, A., Hadj Slama, F., Ben Saïd, M., Zemni, R., & Fathallah, A. (2017). Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton mentagrophytes Strains Isolated from Patients with Dermatophytosis. Mycopathologia, 182(5-6), 487-493. https://doi.org/10.1007/s11046-017-0110-3
Dhib I, et al. Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton Mentagrophytes Strains Isolated From Patients With Dermatophytosis. Mycopathologia. 2017;182(5-6):487-493. PubMed PMID: 28101694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship Between Phenotypic and Genotypic Characteristics of Trichophyton mentagrophytes Strains Isolated from Patients with Dermatophytosis. AU - Dhib,I, AU - Khammari,I, AU - Yaacoub,A, AU - Hadj Slama,F, AU - Ben Saïd,M, AU - Zemni,R, AU - Fathallah,A, Y1 - 2017/01/18/ PY - 2016/10/15/received PY - 2017/01/04/accepted PY - 2017/1/20/pubmed PY - 2018/2/6/medline PY - 2017/1/20/entrez KW - Dermatophytes KW - PCR–RFLP KW - Phenotypic features KW - Sequencing KW - Trichophyton mentagrophytes SP - 487 EP - 493 JF - Mycopathologia JO - Mycopathologia VL - 182 IS - 5-6 N2 - According to epidemiological, clinical and mycological criteria, it has long been admitted that the Trichophyton mentagrophytes species includes two varieties: a zoophilic variety (var. mentagrophytes) and an anthropophilic variety (var. interdigitale) that involve the upper and the lower part of the body, respectively. The further application of molecular techniques to the characterization of dermatophyte strains showed that this classification is unreliable. The aim of our study was to assess the usefulness of PCR-RFLP (restriction fragment length polymorphism) and sequencing in the characterization of T. mentagrophytes strains taken from Tunisian patients. The study was carried out in 2008 in the laboratory of Parasitology-Mycology of Farhat Hached University Hospital, Sousse, Tunisia. A total of 133 strains were isolated from 133 patients addressed to the laboratory for dermatological lesions very evocative of dermatomycosis. Eighty strains were isolated from lesions located on the lower part of the body (onychomycosis, tinea pedis) and 53 strains from the upper part of the body (tinea capitis, tinea corporis). All strains were submitted to mycological examination (direct microscopic examination and culture on Sabouraud medium) and further investigated by using RFLP analysis of the PCR-amplified ITS1-5.8 s-ITS2 region of the ribosomal DNA and the MvaI restriction enzyme. In addition, 62 strains were further submitted to a sequencing of the ITS1-5.8 s-ITS2 region. On the basis of mycological criteria, all strains were diagnosed as T. mentagrophytes. All strains produced the same RFLP pattern and were identified as T. mentagrophytes interdigitale regardless of the location of lesions. Out of the 62 sequenced strains, 16 were found anthropophilic and 46 were zoophilic. In conclusion, all strains provisionally diagnosed as T. mentagrophytes on the basis of mycological criteria were shown to belong to T. interdigitale by using PCR-RFLP and sequencing irrespective of the site of lesions. The predominance of zoophilic strains needs further investigation. SN - 1573-0832 UR - https://www.unboundmedicine.com/medline/citation/28101694/Relationship_Between_Phenotypic_and_Genotypic_Characteristics_of_Trichophyton_mentagrophytes_Strains_Isolated_from_Patients_with_Dermatophytosis_ L2 - https://dx.doi.org/10.1007/s11046-017-0110-3 DB - PRIME DP - Unbound Medicine ER -