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Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling.
Bioanalysis. 2017 Feb; 9(4):349-357.B

Abstract

AIM

Volumetric absorptive microsampler (VAMS) was designed to sample a fixed volume of blood regardless of the hematocrit (HCT) levels. Model compounds with a wide range of hydrophobicity were evaluated for their extraction recoveries from VAMS dried blood samples.

RESULTS

For the highly hydrophobic compounds, recoveries with methanol or methanol/acetonitrile extraction were higher compared with using the aqueous mixture of methanol or acetonitrile. Extraction with methanol/acetonitrile (1:1) yielded more consistent recovery across the HCT range of 20-70% than using methanol alone, with good linearity, accuracy and precision achieved from 1 to 2000 ng/ml.

CONCLUSION

An organic solvent sample preparation approach was developed to optimize extraction recovery and minimize the HCT effect on the analysis of VAMS dried blood samples.

Authors+Show Affiliations

Drug Metabolism & Pharmacokinetics, Vertex Pharmaceuticals, Inc. Boston, MA 02210, USA.Drug Metabolism & Pharmacokinetics, Vertex Pharmaceuticals, Inc. Boston, MA 02210, USA.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28102694

Citation

Ye, Zhengqi, and Hong Gao. "Evaluation of Sample Extraction Methods for Minimizing Hematocrit Effect On Whole Blood Analysis With Volumetric Absorptive Microsampling." Bioanalysis, vol. 9, no. 4, 2017, pp. 349-357.
Ye Z, Gao H. Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling. Bioanalysis. 2017;9(4):349-357.
Ye, Z., & Gao, H. (2017). Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling. Bioanalysis, 9(4), 349-357. https://doi.org/10.4155/bio-2015-0028
Ye Z, Gao H. Evaluation of Sample Extraction Methods for Minimizing Hematocrit Effect On Whole Blood Analysis With Volumetric Absorptive Microsampling. Bioanalysis. 2017;9(4):349-357. PubMed PMID: 28102694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling. AU - Ye,Zhengqi, AU - Gao,Hong, Y1 - 2017/01/19/ PY - 2017/1/20/pubmed PY - 2017/3/8/medline PY - 2017/1/20/entrez KW - hematocrit KW - microsampling KW - volumetric absorptive microsampler SP - 349 EP - 357 JF - Bioanalysis JO - Bioanalysis VL - 9 IS - 4 N2 - AIM: Volumetric absorptive microsampler (VAMS) was designed to sample a fixed volume of blood regardless of the hematocrit (HCT) levels. Model compounds with a wide range of hydrophobicity were evaluated for their extraction recoveries from VAMS dried blood samples. RESULTS: For the highly hydrophobic compounds, recoveries with methanol or methanol/acetonitrile extraction were higher compared with using the aqueous mixture of methanol or acetonitrile. Extraction with methanol/acetonitrile (1:1) yielded more consistent recovery across the HCT range of 20-70% than using methanol alone, with good linearity, accuracy and precision achieved from 1 to 2000 ng/ml. CONCLUSION: An organic solvent sample preparation approach was developed to optimize extraction recovery and minimize the HCT effect on the analysis of VAMS dried blood samples. SN - 1757-6199 UR - https://www.unboundmedicine.com/medline/citation/28102694/Evaluation_of_sample_extraction_methods_for_minimizing_hematocrit_effect_on_whole_blood_analysis_with_volumetric_absorptive_microsampling_ L2 - https://www.future-science.com/doi/full/10.4155/bio-2015-0028?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -