Tags

Type your tag names separated by a space and hit enter

Overexpression of MoSM1, encoding for an immunity-inducing protein from Magnaporthe oryzae, in rice confers broad-spectrum resistance against fungal and bacterial diseases.
Sci Rep 2017; 7:41037SR

Abstract

Potential of MoSM1, encoding for a cerato-platanin protein from Magnaporthe oryzae, in improvement of rice disease resistance was examined. Transient expression of MoSM1 in rice leaves initiated hypersensitive response and upregulated expression of defense genes. When transiently expressed in tobacco leaves, MoSM1 targeted to plasma membrane. The MoSM1-overexpressing (MoSM1-OE) transgenic rice lines showed an improved resistance, as revealed by the reduced disease severity and decreased in planta pathogen growth, against 2 strains belonging to two different races of M. oryzae, causing blast disease, and against 2 strains of Xanthomonas oryzae pv. oryzae, causing bacterial leaf blight disease. However, no alteration in resistance to sheath blight disease was observed in MoSM1-OE lines. The MoSM1-OE plants contained elevated levels of salicylic acid (SA) and jasmonic acid (JA) and constitutively activated the expression of SA and JA signaling-related regulatory and defense genes. Furthermore, the MoSM1-OE plants had no effect on drought and salt stress tolerance and on grain yield. We conclude that MoSM1 confers a broad-spectrum resistance against different pathogens through modulating SA- and JA-mediated signaling pathways without any penalty on abiotic stress tolerance and grain yield, providing a promising potential for application of MoSM1 in improvement of disease resistance in crops.

Authors+Show Affiliations

National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28106116

Citation

Hong, Yongbo, et al. "Overexpression of MoSM1, Encoding for an Immunity-inducing Protein From Magnaporthe Oryzae, in Rice Confers Broad-spectrum Resistance Against Fungal and Bacterial Diseases." Scientific Reports, vol. 7, 2017, p. 41037.
Hong Y, Yang Y, Zhang H, et al. Overexpression of MoSM1, encoding for an immunity-inducing protein from Magnaporthe oryzae, in rice confers broad-spectrum resistance against fungal and bacterial diseases. Sci Rep. 2017;7:41037.
Hong, Y., Yang, Y., Zhang, H., Huang, L., Li, D., & Song, F. (2017). Overexpression of MoSM1, encoding for an immunity-inducing protein from Magnaporthe oryzae, in rice confers broad-spectrum resistance against fungal and bacterial diseases. Scientific Reports, 7, p. 41037. doi:10.1038/srep41037.
Hong Y, et al. Overexpression of MoSM1, Encoding for an Immunity-inducing Protein From Magnaporthe Oryzae, in Rice Confers Broad-spectrum Resistance Against Fungal and Bacterial Diseases. Sci Rep. 2017 01 20;7:41037. PubMed PMID: 28106116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of MoSM1, encoding for an immunity-inducing protein from Magnaporthe oryzae, in rice confers broad-spectrum resistance against fungal and bacterial diseases. AU - Hong,Yongbo, AU - Yang,Yayun, AU - Zhang,Huijuan, AU - Huang,Lei, AU - Li,Dayong, AU - Song,Fengming, Y1 - 2017/01/20/ PY - 2016/04/08/received PY - 2016/12/15/accepted PY - 2017/1/21/entrez PY - 2017/1/21/pubmed PY - 2018/10/23/medline SP - 41037 EP - 41037 JF - Scientific reports JO - Sci Rep VL - 7 N2 - Potential of MoSM1, encoding for a cerato-platanin protein from Magnaporthe oryzae, in improvement of rice disease resistance was examined. Transient expression of MoSM1 in rice leaves initiated hypersensitive response and upregulated expression of defense genes. When transiently expressed in tobacco leaves, MoSM1 targeted to plasma membrane. The MoSM1-overexpressing (MoSM1-OE) transgenic rice lines showed an improved resistance, as revealed by the reduced disease severity and decreased in planta pathogen growth, against 2 strains belonging to two different races of M. oryzae, causing blast disease, and against 2 strains of Xanthomonas oryzae pv. oryzae, causing bacterial leaf blight disease. However, no alteration in resistance to sheath blight disease was observed in MoSM1-OE lines. The MoSM1-OE plants contained elevated levels of salicylic acid (SA) and jasmonic acid (JA) and constitutively activated the expression of SA and JA signaling-related regulatory and defense genes. Furthermore, the MoSM1-OE plants had no effect on drought and salt stress tolerance and on grain yield. We conclude that MoSM1 confers a broad-spectrum resistance against different pathogens through modulating SA- and JA-mediated signaling pathways without any penalty on abiotic stress tolerance and grain yield, providing a promising potential for application of MoSM1 in improvement of disease resistance in crops. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28106116/Overexpression_of_MoSM1_encoding_for_an_immunity_inducing_protein_from_Magnaporthe_oryzae_in_rice_confers_broad_spectrum_resistance_against_fungal_and_bacterial_diseases_ L2 - http://dx.doi.org/10.1038/srep41037 DB - PRIME DP - Unbound Medicine ER -