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Identification of a novel protein kinase that affects the chronological lifespan in fission yeast.
FEMS Microbiol Lett. 2017 01; 364(2)FM

Abstract

Chronological lifespan is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into the stationary phase. To understand the regulatory mechanisms of chronological lifespan in Schizosaccharomyces pombe, it is necessary to identify and characterize novel factors involved in the regulation of chronological lifespan. To this end, we have screened for a long-lived mutant and identified that novel gene nnk1+ that encodes an essential protein kinase is the determinant of chronological lifespan. We showed that the expression of major glucose transporter gene, ght5+, is decreased in the isolated nnk1-35 mutant, suggesting that Nnk1 protein is involved in the regulation of ght5+ The consumption of glucose in the growth medium after saturated growth was lower in the nnk1-35 mutant than that in wild-type cell. The isolated ght5 deletion mutant showed long-lived phenotype. Based on these results, we propose that Nnk1 regulates chronological lifespan through the regulation of ght5+ Nnk1 might coordinate glucose availability and lifespan in fission yeast.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan aiba@ps.nagoya-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28108582

Citation

Kurauchi, Tatsuhiro, et al. "Identification of a Novel Protein Kinase That Affects the Chronological Lifespan in Fission Yeast." FEMS Microbiology Letters, vol. 364, no. 2, 2017.
Kurauchi T, Hashizume A, Imai Y, et al. Identification of a novel protein kinase that affects the chronological lifespan in fission yeast. FEMS Microbiol Lett. 2017;364(2).
Kurauchi, T., Hashizume, A., Imai, Y., Hayashi, K., Tsubouchi, S., Ihara, K., Ohtsuka, H., & Aiba, H. (2017). Identification of a novel protein kinase that affects the chronological lifespan in fission yeast. FEMS Microbiology Letters, 364(2). https://doi.org/10.1093/femsle/fnw257
Kurauchi T, et al. Identification of a Novel Protein Kinase That Affects the Chronological Lifespan in Fission Yeast. FEMS Microbiol Lett. 2017;364(2) PubMed PMID: 28108582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a novel protein kinase that affects the chronological lifespan in fission yeast. AU - Kurauchi,Tatsuhiro, AU - Hashizume,Aya, AU - Imai,Yuki, AU - Hayashi,Kanako, AU - Tsubouchi,Satoshi, AU - Ihara,Kunio, AU - Ohtsuka,Hokuto, AU - Aiba,Hirofumi, Y1 - 2016/11/09/ PY - 2016/11/08/accepted PY - 2016/09/24/revised PY - 2017/1/22/entrez PY - 2017/1/22/pubmed PY - 2017/5/10/medline KW - Lifespan KW - Nnk1 KW - Schizosaccharomyces pombe KW - aging, glucose transporter KW - yeast JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 364 IS - 2 N2 - Chronological lifespan is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into the stationary phase. To understand the regulatory mechanisms of chronological lifespan in Schizosaccharomyces pombe, it is necessary to identify and characterize novel factors involved in the regulation of chronological lifespan. To this end, we have screened for a long-lived mutant and identified that novel gene nnk1+ that encodes an essential protein kinase is the determinant of chronological lifespan. We showed that the expression of major glucose transporter gene, ght5+, is decreased in the isolated nnk1-35 mutant, suggesting that Nnk1 protein is involved in the regulation of ght5+ The consumption of glucose in the growth medium after saturated growth was lower in the nnk1-35 mutant than that in wild-type cell. The isolated ght5 deletion mutant showed long-lived phenotype. Based on these results, we propose that Nnk1 regulates chronological lifespan through the regulation of ght5+ Nnk1 might coordinate glucose availability and lifespan in fission yeast. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/28108582/Identification_of_a_novel_protein_kinase_that_affects_the_chronological_lifespan_in_fission_yeast_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1093/femsle/fnw257 DB - PRIME DP - Unbound Medicine ER -