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Differences in Monoterpene Biosynthesis and Accumulation in Pistacia palaestina Leaves and Aphid-Induced Galls.
J Chem Ecol. 2017 Feb; 43(2):143-152.JC

Abstract

Certain insect species can induce gall formation on numerous plants species. Although the mechanism of gall development is largely unknown, it is clear that insects manipulate their hosts' anatomy, physiology, and chemistry for their own benefit. It is well known that insect-induced galls often contain vast amounts of plant defensive compounds as compared to non-colonized tissues, but it is not clear if defensive compounds can be produced in situ in the galled tissues. To answer this question, we analyzed terpene accumulation patterns and possible independent biosynthetic potential of galls induced by the aphid Baizongia pistaciae L. on the terminal buds of Pistacia palaestina Boiss. We compared monoterpene levels and monoterpene synthase enzyme activity in galls and healthy leaves from individual trees growing in a natural setting. At all developmental stages, monoterpene content and monoterpene synthase activity were consistently (up to 10 fold on a fresh weight basis) higher in galls than in intact non-colonized leaves. A remarkable tree to tree variation in the products produced in vitro from the substrate geranyl diphosphate by soluble protein extracts derived from individual trees was observed. Furthermore, galls and leaves from the same trees displayed enhanced and often distinct biosynthetic capabilities. Our results clearly indicate that galls possess independent metabolic capacities to produce and accumulate monoterpenes as compared to leaves. Our study indicates that galling aphids manipulate the enzymatic machinery of their host plant, intensifying their own defenses against natural enemies.

Authors+Show Affiliations

Department of Evolutionary & Environmental Biology, University of Haifa, Mount Carmel, 31905, Haifa, Israel. Newe Ya'ar Research Center, Agricultural Research Organization, Institute of Plant Sciences, 30095, Ramat Yishay, Israel.Newe Ya'ar Research Center, Agricultural Research Organization, Institute of Plant Sciences, 30095, Ramat Yishay, Israel.Department of Evolutionary & Environmental Biology, University of Haifa, Mount Carmel, 31905, Haifa, Israel.Newe Ya'ar Research Center, Agricultural Research Organization, Institute of Plant Sciences, 30095, Ramat Yishay, Israel.Department of Biochemistry, Purdue University, West Lafayette, IN, 47907-1165, USA.Department of Evolutionary & Environmental Biology, University of Haifa, Mount Carmel, 31905, Haifa, Israel.Newe Ya'ar Research Center, Agricultural Research Organization, Institute of Plant Sciences, 30095, Ramat Yishay, Israel. twefraim@agri.gov.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28108840

Citation

Rand, Karin, et al. "Differences in Monoterpene Biosynthesis and Accumulation in Pistacia Palaestina Leaves and Aphid-Induced Galls." Journal of Chemical Ecology, vol. 43, no. 2, 2017, pp. 143-152.
Rand K, Bar E, Ari MB, et al. Differences in Monoterpene Biosynthesis and Accumulation in Pistacia palaestina Leaves and Aphid-Induced Galls. J Chem Ecol. 2017;43(2):143-152.
Rand, K., Bar, E., Ari, M. B., Davidovich-Rikanati, R., Dudareva, N., Inbar, M., & Lewinsohn, E. (2017). Differences in Monoterpene Biosynthesis and Accumulation in Pistacia palaestina Leaves and Aphid-Induced Galls. Journal of Chemical Ecology, 43(2), 143-152. https://doi.org/10.1007/s10886-016-0817-5
Rand K, et al. Differences in Monoterpene Biosynthesis and Accumulation in Pistacia Palaestina Leaves and Aphid-Induced Galls. J Chem Ecol. 2017;43(2):143-152. PubMed PMID: 28108840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in Monoterpene Biosynthesis and Accumulation in Pistacia palaestina Leaves and Aphid-Induced Galls. AU - Rand,Karin, AU - Bar,Einat, AU - Ari,Matan Ben, AU - Davidovich-Rikanati,Rachel, AU - Dudareva,Natalia, AU - Inbar,Moshe, AU - Lewinsohn,Efraim, Y1 - 2017/01/20/ PY - 2016/08/22/received PY - 2016/12/20/accepted PY - 2016/11/23/revised PY - 2017/1/22/pubmed PY - 2017/3/25/medline PY - 2017/1/22/entrez KW - Defensive compounds KW - Host manipulation KW - Insect galls KW - Monoterpene synthase KW - Monoterpenes SP - 143 EP - 152 JF - Journal of chemical ecology JO - J Chem Ecol VL - 43 IS - 2 N2 - Certain insect species can induce gall formation on numerous plants species. Although the mechanism of gall development is largely unknown, it is clear that insects manipulate their hosts' anatomy, physiology, and chemistry for their own benefit. It is well known that insect-induced galls often contain vast amounts of plant defensive compounds as compared to non-colonized tissues, but it is not clear if defensive compounds can be produced in situ in the galled tissues. To answer this question, we analyzed terpene accumulation patterns and possible independent biosynthetic potential of galls induced by the aphid Baizongia pistaciae L. on the terminal buds of Pistacia palaestina Boiss. We compared monoterpene levels and monoterpene synthase enzyme activity in galls and healthy leaves from individual trees growing in a natural setting. At all developmental stages, monoterpene content and monoterpene synthase activity were consistently (up to 10 fold on a fresh weight basis) higher in galls than in intact non-colonized leaves. A remarkable tree to tree variation in the products produced in vitro from the substrate geranyl diphosphate by soluble protein extracts derived from individual trees was observed. Furthermore, galls and leaves from the same trees displayed enhanced and often distinct biosynthetic capabilities. Our results clearly indicate that galls possess independent metabolic capacities to produce and accumulate monoterpenes as compared to leaves. Our study indicates that galling aphids manipulate the enzymatic machinery of their host plant, intensifying their own defenses against natural enemies. SN - 1573-1561 UR - https://www.unboundmedicine.com/medline/citation/28108840/Differences_in_Monoterpene_Biosynthesis_and_Accumulation_in_Pistacia_palaestina_Leaves_and_Aphid_Induced_Galls_ L2 - https://doi.org/10.1007/s10886-016-0817-5 DB - PRIME DP - Unbound Medicine ER -