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The selective cytotoxicity of DSF-Cu attributes to the biomechanical properties and cytoskeleton rearrangements in the normal and cancerous nasopharyngeal epithelial cells.
Int J Biochem Cell Biol 2017; 84:96-108IJ

Abstract

Cancer initiation and progression follow complex changes of cellular architecture and biomechanical property. Cancer cells with more submissive (or "softer") than their healthy counterparts attributed to the reorganization of the complex cytoskeleton structure, may be considered as a potential anti-tumor therapeutic target. In this study, atomic force microscopy (AFM) was carried out to detect the topographical and biophysical changes of nasopharyngeal carcinoma CNE-2Z cells and normal nasopharyngeal epithelial cells NP69-SV40T by treating the Disulfiram chelated with Cu2+ (DSF-Cu). DSF-Cu induced the apoptotic population, ROS production and decreased the NF-κB-p65 expression of CNE-2Z cells, which was much higher than those of NP69-SV40T cells. DSF-Cu caused the obvious changes of cell morphology and membrane ultrastructure in CNE-2Z cells. The roughness decreased and stiffness increased significantly in CNE-2Z cells, which correlated with the rearrangement of intracellular F-actin, FLNa and α-tubulin structures in CNE-2Z cells. And the adhesion force of CNE-2Z cells was also increased accompanied with the increased E-cadherin expression. However, these results could not be observed in the NP69-SV40T cells even the concentration of DSF reached up to 400nM. Finally, the detection of cell wound scratch assay confirmed DSF-Cu could inhibit the migration of CNE-2Z cells, but no effect on NP69-SV40T cells. These findings demonstrated the selective cytotoxicity of DSF-Cu in CNE-2Z cells may attribute to the different mechanical properties and cytoskeleton rearrangement from the normal nasopharyngeal epithelial cells.

Authors+Show Affiliations

Analysis and Test Center, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China; Institute of Clinical Pharmacy, Beijing Municipal Health Bureau, Beijing 100035, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Analysis and Test Center, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Electronic address: linyanzhu_jnu@163.com.Department of Pathology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou 510120, China. Electronic address: yang_hf@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28111334

Citation

Yang, Yaping, et al. "The Selective Cytotoxicity of DSF-Cu Attributes to the Biomechanical Properties and Cytoskeleton Rearrangements in the Normal and Cancerous Nasopharyngeal Epithelial Cells." The International Journal of Biochemistry & Cell Biology, vol. 84, 2017, pp. 96-108.
Yang Y, Li M, Sun X, et al. The selective cytotoxicity of DSF-Cu attributes to the biomechanical properties and cytoskeleton rearrangements in the normal and cancerous nasopharyngeal epithelial cells. Int J Biochem Cell Biol. 2017;84:96-108.
Yang, Y., Li, M., Sun, X., Zhou, C., Wang, Y., Wang, L., ... Yang, H. (2017). The selective cytotoxicity of DSF-Cu attributes to the biomechanical properties and cytoskeleton rearrangements in the normal and cancerous nasopharyngeal epithelial cells. The International Journal of Biochemistry & Cell Biology, 84, pp. 96-108. doi:10.1016/j.biocel.2017.01.007.
Yang Y, et al. The Selective Cytotoxicity of DSF-Cu Attributes to the Biomechanical Properties and Cytoskeleton Rearrangements in the Normal and Cancerous Nasopharyngeal Epithelial Cells. Int J Biochem Cell Biol. 2017;84:96-108. PubMed PMID: 28111334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The selective cytotoxicity of DSF-Cu attributes to the biomechanical properties and cytoskeleton rearrangements in the normal and cancerous nasopharyngeal epithelial cells. AU - Yang,Yaping, AU - Li,Mengjia, AU - Sun,Xiaoxue, AU - Zhou,Congran, AU - Wang,Yawei, AU - Wang,Liwei, AU - Chen,Lixin, AU - Liang,Zhihong, AU - Zhu,Linyan, AU - Yang,Haifeng, Y1 - 2017/01/19/ PY - 2016/09/11/received PY - 2017/01/14/revised PY - 2017/01/16/accepted PY - 2017/1/24/pubmed PY - 2017/11/3/medline PY - 2017/1/24/entrez KW - Adhesion force KW - Atomic force microscopy KW - Cell stiffness KW - Cytoskeletal reorganization KW - Nasopharyngeal carcinoma cells SP - 96 EP - 108 JF - The international journal of biochemistry & cell biology JO - Int. J. Biochem. Cell Biol. VL - 84 N2 - Cancer initiation and progression follow complex changes of cellular architecture and biomechanical property. Cancer cells with more submissive (or "softer") than their healthy counterparts attributed to the reorganization of the complex cytoskeleton structure, may be considered as a potential anti-tumor therapeutic target. In this study, atomic force microscopy (AFM) was carried out to detect the topographical and biophysical changes of nasopharyngeal carcinoma CNE-2Z cells and normal nasopharyngeal epithelial cells NP69-SV40T by treating the Disulfiram chelated with Cu2+ (DSF-Cu). DSF-Cu induced the apoptotic population, ROS production and decreased the NF-κB-p65 expression of CNE-2Z cells, which was much higher than those of NP69-SV40T cells. DSF-Cu caused the obvious changes of cell morphology and membrane ultrastructure in CNE-2Z cells. The roughness decreased and stiffness increased significantly in CNE-2Z cells, which correlated with the rearrangement of intracellular F-actin, FLNa and α-tubulin structures in CNE-2Z cells. And the adhesion force of CNE-2Z cells was also increased accompanied with the increased E-cadherin expression. However, these results could not be observed in the NP69-SV40T cells even the concentration of DSF reached up to 400nM. Finally, the detection of cell wound scratch assay confirmed DSF-Cu could inhibit the migration of CNE-2Z cells, but no effect on NP69-SV40T cells. These findings demonstrated the selective cytotoxicity of DSF-Cu in CNE-2Z cells may attribute to the different mechanical properties and cytoskeleton rearrangement from the normal nasopharyngeal epithelial cells. SN - 1878-5875 UR - https://www.unboundmedicine.com/medline/citation/28111334/The_selective_cytotoxicity_of_DSF_Cu_attributes_to_the_biomechanical_properties_and_cytoskeleton_rearrangements_in_the_normal_and_cancerous_nasopharyngeal_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1357-2725(17)30014-6 DB - PRIME DP - Unbound Medicine ER -