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Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat.
Theor Appl Genet. 2017 Apr; 130(4):841-848.TA

Abstract

KEY MESSAGE

Pm57, a novel resistant gene against powdery mildew, was transferred into common wheat from Ae. searsi and further mapped to 2S s #1L at an interval of FL0.75 to FL0.87. Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici, is one of the most severe foliar diseases of wheat causing reduction in grain yield and quality. Host plant resistance is the most effective and environmentally safe approach to control this disease. Tests of a set of Chinese Spring-Ae. searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer disomic addition lines with a mixed isolate of the powdery mildew fungus identified a novel resistance gene(s), designed as Pm57, which was located on chromosome 2Ss#1. Here, we report the development of ten wheat-Ae. searsii recombinants. The wheat chromosomes involved in five of these recombinants were identified by FISH and SSR marker analysis and three of them were resistant to powdery mildew. Pm57 was further mapped to the long arm of chromosome 2Ss#1 at a fraction length interval of FL 0.75 to FL 0.87. The recombinant stocks T2BS.2BL-2Ss#1L 89-346 (TA5108) with distal 2Ss#1L segments of 28% and 89(5)69 (TA5109) with 33% may be useful in wheat improvement. The PCR marker X2L4g9p4/HaeIII was validated to specifically identify the Ae. searsii 2Ss#1L segment harboring Pm57 in T2BS.2BL-2Ss#1L against 16 wheat varieties and advanced breeding lines, and the development of more user-friendly KASP markers is underway.

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Authors+Show Affiliations

Liu W
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. wxliu2003@hotmail.com.
Koo DH
Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
Xia Q
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
Li C
Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
Bai F
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
Song Y
Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
Friebe B
Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
Gill BS
Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.

MeSH

AscomycotaChromosome MappingChromosomes, PlantDNA, PlantDisease ResistanceGenes, PlantGenetic MarkersPlant DiseasesPlants, Genetically ModifiedPoaceaeRecombination, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28116459

Citation

Liu, Wenxuan, et al. "Homoeologous Recombination-based Transfer and Molecular Cytogenetic Mapping of Powdery Mildew-resistant Gene Pm57 From Aegilops Searsii Into Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 4, 2017, pp. 841-848.
Liu W, Koo DH, Xia Q, et al. Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat. Theor Appl Genet. 2017;130(4):841-848.
Liu, W., Koo, D. H., Xia, Q., Li, C., Bai, F., Song, Y., Friebe, B., & Gill, B. S. (2017). Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(4), 841-848. https://doi.org/10.1007/s00122-017-2855-y
Liu W, et al. Homoeologous Recombination-based Transfer and Molecular Cytogenetic Mapping of Powdery Mildew-resistant Gene Pm57 From Aegilops Searsii Into Wheat. Theor Appl Genet. 2017;130(4):841-848. PubMed PMID: 28116459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat. AU - Liu,Wenxuan, AU - Koo,Dal-Hoe, AU - Xia,Qing, AU - Li,Chunxin, AU - Bai,Fuqiang, AU - Song,Yuli, AU - Friebe,Bernd, AU - Gill,Bikram S, Y1 - 2017/01/23/ PY - 2016/11/09/received PY - 2017/01/04/accepted PY - 2017/1/25/pubmed PY - 2017/4/8/medline PY - 2017/1/25/entrez KW - Aegilops searsii KW - Blumeria tritici KW - FISH KW - GISH KW - Pm57 KW - Triticum aestivum SP - 841 EP - 848 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 4 N2 - KEY MESSAGE: Pm57, a novel resistant gene against powdery mildew, was transferred into common wheat from Ae. searsi and further mapped to 2S s #1L at an interval of FL0.75 to FL0.87. Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici, is one of the most severe foliar diseases of wheat causing reduction in grain yield and quality. Host plant resistance is the most effective and environmentally safe approach to control this disease. Tests of a set of Chinese Spring-Ae. searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer disomic addition lines with a mixed isolate of the powdery mildew fungus identified a novel resistance gene(s), designed as Pm57, which was located on chromosome 2Ss#1. Here, we report the development of ten wheat-Ae. searsii recombinants. The wheat chromosomes involved in five of these recombinants were identified by FISH and SSR marker analysis and three of them were resistant to powdery mildew. Pm57 was further mapped to the long arm of chromosome 2Ss#1 at a fraction length interval of FL 0.75 to FL 0.87. The recombinant stocks T2BS.2BL-2Ss#1L 89-346 (TA5108) with distal 2Ss#1L segments of 28% and 89(5)69 (TA5109) with 33% may be useful in wheat improvement. The PCR marker X2L4g9p4/HaeIII was validated to specifically identify the Ae. searsii 2Ss#1L segment harboring Pm57 in T2BS.2BL-2Ss#1L against 16 wheat varieties and advanced breeding lines, and the development of more user-friendly KASP markers is underway. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28116459/Homoeologous_recombination_based_transfer_and_molecular_cytogenetic_mapping_of_powdery_mildew_resistant_gene_Pm57_from_Aegilops_searsii_into_wheat_ DB - PRIME DP - Unbound Medicine ER -