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Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.
Sci Rep. 2017 01 25; 7:40685.SR

Abstract

Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn2+) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO2- at 1240 cm-1 and stretching of C-O ribose at 1060 cm-1, suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn2+ in plants.

Authors+Show Affiliations

Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002 UP, India.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002 UP, India. Department of Applied Physics, Aligarh Muslim University, Aligarh, 202002 UP, India.Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, India.Department of Applied Physics, Aligarh Muslim University, Aligarh, 202002 UP, India.Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002 UP, India.Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002 UP, India. School of Biosciences and Biodiversity, Baba Ghulam Shah Badshah University, Rajouri, J &K, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28120857

Citation

Ahmed, Bilal, et al. "Mitochondrial and Chromosomal Damage Induced By Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs Treated Allium Cepa Roots." Scientific Reports, vol. 7, 2017, p. 40685.
Ahmed B, Dwivedi S, Abdin MZ, et al. Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots. Sci Rep. 2017;7:40685.
Ahmed, B., Dwivedi, S., Abdin, M. Z., Azam, A., Al-Shaeri, M., Khan, M. S., Saquib, Q., Al-Khedhairy, A. A., & Musarrat, J. (2017). Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots. Scientific Reports, 7, 40685. https://doi.org/10.1038/srep40685
Ahmed B, et al. Mitochondrial and Chromosomal Damage Induced By Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs Treated Allium Cepa Roots. Sci Rep. 2017 01 25;7:40685. PubMed PMID: 28120857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots. AU - Ahmed,Bilal, AU - Dwivedi,Sourabh, AU - Abdin,Malik Zainul, AU - Azam,Ameer, AU - Al-Shaeri,Majed, AU - Khan,Mohammad Saghir, AU - Saquib,Quaiser, AU - Al-Khedhairy,Abdulaziz A, AU - Musarrat,Javed, Y1 - 2017/01/25/ PY - 2016/08/12/received PY - 2016/12/09/accepted PY - 2017/1/26/entrez PY - 2017/1/26/pubmed PY - 2018/10/20/medline SP - 40685 EP - 40685 JF - Scientific reports JO - Sci Rep VL - 7 N2 - Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn2+) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO2- at 1240 cm-1 and stretching of C-O ribose at 1060 cm-1, suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn2+ in plants. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28120857/Mitochondrial_and_Chromosomal_Damage_Induced_by_Oxidative_Stress_in_Zn2+_Ions_ZnO_Bulk_and_ZnO_NPs_treated_Allium_cepa_roots_ L2 - https://doi.org/10.1038/srep40685 DB - PRIME DP - Unbound Medicine ER -