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Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model.
Iran J Parasitol 2016 Jul-Sep; 11(3):383-388IJ

Abstract

BACKGROUND

This investigation aimed to evaluate the in vitro scolicidal effects of Pistacia khinjuk methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model.

METHODS

Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5-100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of P. khinjuk.

RESULTS

P. khinjuk extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD50 of the intraperitoneal injection of the P. khinjuk methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg.

CONCLUSION

The findings demonstrated effective scolicidal effects of P. khinjuk extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent.

Authors+Show Affiliations

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28127345

Citation

Mahmoudvand, Hossein, et al. "Efficacy of Pistacia Khinjuk Fruits On Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model." Iranian Journal of Parasitology, vol. 11, no. 3, 2016, pp. 383-388.
Mahmoudvand H, Mirbadie SR, Ghasemi Kia M, et al. Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model. Iran J Parasitol. 2016;11(3):383-388.
Mahmoudvand, H., Mirbadie, S. R., Ghasemi Kia, M., Badparva, E., Shamsadini Lori, S., & Fasihi Harandi, M. (2016). Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model. Iranian Journal of Parasitology, 11(3), pp. 383-388.
Mahmoudvand H, et al. Efficacy of Pistacia Khinjuk Fruits On Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model. Iran J Parasitol. 2016;11(3):383-388. PubMed PMID: 28127345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model. AU - Mahmoudvand,Hossein, AU - Mirbadie,Seyed Reza, AU - Ghasemi Kia,Mehdi, AU - Badparva,Ebrahim, AU - Shamsadini Lori,Saeedeh, AU - Fasihi Harandi,Majid, PY - 2017/1/28/entrez PY - 2017/1/28/pubmed PY - 2017/1/28/medline KW - Cystic echinococcosis KW - Echinococcus granulosus KW - Hydatid cyst KW - Scolicidal KW - Toxicity SP - 383 EP - 388 JF - Iranian journal of parasitology JO - Iran J Parasitol VL - 11 IS - 3 N2 - BACKGROUND: This investigation aimed to evaluate the in vitro scolicidal effects of Pistacia khinjuk methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model. METHODS: Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5-100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of P. khinjuk. RESULTS: P. khinjuk extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD50 of the intraperitoneal injection of the P. khinjuk methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg. CONCLUSION: The findings demonstrated effective scolicidal effects of P. khinjuk extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent. SN - 1735-7020 UR - https://www.unboundmedicine.com/medline/citation/28127345/Efficacy_of_Pistacia_khinjuk_Fruits_on_Viability_of_Hydatid_Cyst_Protoscoleces_and_Its_Acute_Toxicity_in_Mice_Model_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28127345/ DB - PRIME DP - Unbound Medicine ER -