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Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study.
Atherosclerosis. 2017 02; 257:116-122.A

Abstract

BACKGROUND AND AIMS

Whether EPA and DHA exert similar anti-inflammatory effects through modulation of gene expression in immune cells remains unclear. The aim of the study was to compare the impact of EPA and DHA supplementation on inflammatory gene expression in subjects at risk for cardiometabolic diseases.

METHODS

In this randomized double-blind crossover trial, 154 men and women with abdominal obesity and low-grade inflammation were subjected to three 10-wk supplementation phases: 1) EPA (2.7 g/d); 2) DHA (2.7 g/d); 3) corn oil (3 g/d), separated by a 9-wk washout. Pro- and anti-inflammatory gene expression was assessed in whole blood cells by RT-qPCR after each treatment in a representative sample of 44 participants.

RESULTS

No significant difference was observed between EPA and DHA in the expression of any of the genes investigated. Compared with control, EPA enhanced TRAF3 and PPARA expression and lowered CD14 expression (p < 0.01) whereas DHA increased expression of PPARA and TNFA and decreased CD14 expression (p < 0.05). Variations in gene expression after EPA and after DHA were strongly correlated for PPARA (r = 0.73, p < 0.0001) and TRAF3 (r = 0.66, p < 0.0001) and less for TNFA (r = 0.46, p < 0.005) and CD14 (r = 0.16, p = 0.30).

CONCLUSIONS

High-dose supplementation with either EPA or DHA has similar effects on the expression of many inflammation-related genes in immune cells of men and women at risk for cardiometabolic diseases. The effects of EPA and of DHA on anti-inflammatory gene expression may be more consistent than their effects on expression of pro-inflammatory genes in whole blood cells.

Authors+Show Affiliations

Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada; Centre de recherche du CHU de Québec, Université Laval, Québec, Canada; Institut universitaire de cardiologique et de pneumologie du Québec (IUCPQ), Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada; Centre de recherche du CHU de Québec, Université Laval, Québec, Canada.Institut sur la nutrition et les aliments fonctionnels (INAF), Pavillon des Services, Université Laval, Québec, Canada. Electronic address: benoit.lamarche@fsaa.ulaval.ca.

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28131045

Citation

Vors, Cécile, et al. "Inflammatory Gene Expression in Whole Blood Cells After EPA Vs. DHA Supplementation: Results From the ComparED Study." Atherosclerosis, vol. 257, 2017, pp. 116-122.
Vors C, Allaire J, Marin J, et al. Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study. Atherosclerosis. 2017;257:116-122.
Vors, C., Allaire, J., Marin, J., Lépine, M. C., Charest, A., Tchernof, A., Couture, P., & Lamarche, B. (2017). Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study. Atherosclerosis, 257, 116-122. https://doi.org/10.1016/j.atherosclerosis.2017.01.025
Vors C, et al. Inflammatory Gene Expression in Whole Blood Cells After EPA Vs. DHA Supplementation: Results From the ComparED Study. Atherosclerosis. 2017;257:116-122. PubMed PMID: 28131045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study. AU - Vors,Cécile, AU - Allaire,Janie, AU - Marin,Johanne, AU - Lépine,Marie-Claude, AU - Charest,Amélie, AU - Tchernof,André, AU - Couture,Patrick, AU - Lamarche,Benoît, Y1 - 2017/01/20/ PY - 2016/11/24/received PY - 2017/01/13/revised PY - 2017/01/19/accepted PY - 2017/1/29/pubmed PY - 2017/11/2/medline PY - 2017/1/29/entrez KW - Docosahexaenoic acid KW - Eicosapentaenoic acid KW - Immune cells KW - Inflammation KW - Omega-3 KW - Peroxisome proliferator-activated receptor alpha KW - TNF receptor associated factor 3 SP - 116 EP - 122 JF - Atherosclerosis JO - Atherosclerosis VL - 257 N2 - BACKGROUND AND AIMS: Whether EPA and DHA exert similar anti-inflammatory effects through modulation of gene expression in immune cells remains unclear. The aim of the study was to compare the impact of EPA and DHA supplementation on inflammatory gene expression in subjects at risk for cardiometabolic diseases. METHODS: In this randomized double-blind crossover trial, 154 men and women with abdominal obesity and low-grade inflammation were subjected to three 10-wk supplementation phases: 1) EPA (2.7 g/d); 2) DHA (2.7 g/d); 3) corn oil (3 g/d), separated by a 9-wk washout. Pro- and anti-inflammatory gene expression was assessed in whole blood cells by RT-qPCR after each treatment in a representative sample of 44 participants. RESULTS: No significant difference was observed between EPA and DHA in the expression of any of the genes investigated. Compared with control, EPA enhanced TRAF3 and PPARA expression and lowered CD14 expression (p < 0.01) whereas DHA increased expression of PPARA and TNFA and decreased CD14 expression (p < 0.05). Variations in gene expression after EPA and after DHA were strongly correlated for PPARA (r = 0.73, p < 0.0001) and TRAF3 (r = 0.66, p < 0.0001) and less for TNFA (r = 0.46, p < 0.005) and CD14 (r = 0.16, p = 0.30). CONCLUSIONS: High-dose supplementation with either EPA or DHA has similar effects on the expression of many inflammation-related genes in immune cells of men and women at risk for cardiometabolic diseases. The effects of EPA and of DHA on anti-inflammatory gene expression may be more consistent than their effects on expression of pro-inflammatory genes in whole blood cells. SN - 1879-1484 UR - https://www.unboundmedicine.com/medline/citation/28131045/Inflammatory_gene_expression_in_whole_blood_cells_after_EPA_vs__DHA_supplementation:_Results_from_the_ComparED_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9150(17)30027-8 DB - PRIME DP - Unbound Medicine ER -