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Protective effects of ginsenoside Rg1 against oxygen-glucose-deprivation-induced apoptosis in neural stem cells.
J Neurol Sci. 2017 Feb 15; 373:107-112.JN

Abstract

Oxygen-glucose deprivation (OGD) causes neural damages through stroke-induced ischemia. Neural stem cells (NSCs) have been shown to alleviate ischemia-induced neural damages. However, ischemia reduces NSC survival. Ginsenoside Rg1 exerts anti-inflammatory and anti-oxidative effects, and repairs brain injury-related neural damages. We aimed to investigate whether ginsenoside Rg1 could prevent NSCs from OGD insult. Using multiple techniques, we explored neuroprotective effects of ginsenoside Rg1 on OGD-insulted NSCs. 6h treatment of OGD most significantly decreased NSC viability, and 10-20μM ginsenoside Rg1 efficiently protected NSCs against OGD insult. Annexin V-FITC/propidium iodide (PI) double staining results confirmed that ginsenoside Rg1 significantly reduced the OGD-induced apoptosis in NSCs. OGD-insulted NSCs with ginsenoside Rg1 treatment displayed reduced expressions of pro-apoptotic proteins cleaved Caspase3 and Bax, and elevated expression of anti-apoptotic protein Bcl-2 than the NSCs with OGD insult. Moreover, ginsenoside Rg1 reduced OGD-induced oxidative stress, and inhibited the expression of p-p38 and p-JNK2. Ginsenoside Rg1 protects NSCs against OGD-induced cell apoptosis through regulating the expression of apoptotic signal proteins. In addition, ginsenoside Rg1 attenuates OGD-induced oxidative stress and inhibits p38/JNK2 phosphorylation in NSCs. Our study provides solid evidence for neuroprotective effects of ginsenoside Rg1 and reveals the underlying mechanisms.

Authors+Show Affiliations

Department of Pharmacy, China Meitan General Hospital, Xibahenanli 29, Chaoyang District, Beijing 100028, China.Department of Pharmacy, China Meitan General Hospital, Xibahenanli 29, Chaoyang District, Beijing 100028, China.Department of Pharmacy, China Meitan General Hospital, Xibahenanli 29, Chaoyang District, Beijing 100028, China.Department of Pharmacy, China Meitan General Hospital, Xibahenanli 29, Chaoyang District, Beijing 100028, China.Department of Pharmacy, China Meitan General Hospital, Xibahenanli 29, Chaoyang District, Beijing 100028, China. Electronic address: xuewenxinbj@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28131165

Citation

Li, Yiwen, et al. "Protective Effects of Ginsenoside Rg1 Against Oxygen-glucose-deprivation-induced Apoptosis in Neural Stem Cells." Journal of the Neurological Sciences, vol. 373, 2017, pp. 107-112.
Li Y, Suo L, Liu Y, et al. Protective effects of ginsenoside Rg1 against oxygen-glucose-deprivation-induced apoptosis in neural stem cells. J Neurol Sci. 2017;373:107-112.
Li, Y., Suo, L., Liu, Y., Li, H., & Xue, W. (2017). Protective effects of ginsenoside Rg1 against oxygen-glucose-deprivation-induced apoptosis in neural stem cells. Journal of the Neurological Sciences, 373, 107-112. https://doi.org/10.1016/j.jns.2016.12.036
Li Y, et al. Protective Effects of Ginsenoside Rg1 Against Oxygen-glucose-deprivation-induced Apoptosis in Neural Stem Cells. J Neurol Sci. 2017 Feb 15;373:107-112. PubMed PMID: 28131165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of ginsenoside Rg1 against oxygen-glucose-deprivation-induced apoptosis in neural stem cells. AU - Li,Yiwen, AU - Suo,Lin, AU - Liu,Yang, AU - Li,Huiyun, AU - Xue,Wenxin, Y1 - 2016/12/22/ PY - 2016/11/16/received PY - 2016/12/14/revised PY - 2016/12/20/accepted PY - 2017/1/30/entrez PY - 2017/1/31/pubmed PY - 2017/6/1/medline KW - Anti-oxidative KW - Ginsenoside Rg1 KW - Neural stem cells KW - Neuroprotective KW - Oxygen-glucose deprivation SP - 107 EP - 112 JF - Journal of the neurological sciences JO - J. Neurol. Sci. VL - 373 N2 - Oxygen-glucose deprivation (OGD) causes neural damages through stroke-induced ischemia. Neural stem cells (NSCs) have been shown to alleviate ischemia-induced neural damages. However, ischemia reduces NSC survival. Ginsenoside Rg1 exerts anti-inflammatory and anti-oxidative effects, and repairs brain injury-related neural damages. We aimed to investigate whether ginsenoside Rg1 could prevent NSCs from OGD insult. Using multiple techniques, we explored neuroprotective effects of ginsenoside Rg1 on OGD-insulted NSCs. 6h treatment of OGD most significantly decreased NSC viability, and 10-20μM ginsenoside Rg1 efficiently protected NSCs against OGD insult. Annexin V-FITC/propidium iodide (PI) double staining results confirmed that ginsenoside Rg1 significantly reduced the OGD-induced apoptosis in NSCs. OGD-insulted NSCs with ginsenoside Rg1 treatment displayed reduced expressions of pro-apoptotic proteins cleaved Caspase3 and Bax, and elevated expression of anti-apoptotic protein Bcl-2 than the NSCs with OGD insult. Moreover, ginsenoside Rg1 reduced OGD-induced oxidative stress, and inhibited the expression of p-p38 and p-JNK2. Ginsenoside Rg1 protects NSCs against OGD-induced cell apoptosis through regulating the expression of apoptotic signal proteins. In addition, ginsenoside Rg1 attenuates OGD-induced oxidative stress and inhibits p38/JNK2 phosphorylation in NSCs. Our study provides solid evidence for neuroprotective effects of ginsenoside Rg1 and reveals the underlying mechanisms. SN - 1878-5883 UR - https://www.unboundmedicine.com/medline/citation/28131165/Protective_effects_of_ginsenoside_Rg1_against_oxygen_glucose_deprivation_induced_apoptosis_in_neural_stem_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-510X(16)30829-2 DB - PRIME DP - Unbound Medicine ER -