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Wound healing potential of adipose tissue stem cell extract.
Biochem Biophys Res Commun. 2017 03 25; 485(1):30-34.BB

Abstract

Adipose tissue stem cells (ATSCs) are considered as a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using stem cells, intercellular molecule exchange by stem cell secretory factors showed beneficial effects by reducing tissue damage and augmentation of endogenous repair. Delayed cutaneous wound healing is implicated in many conditions such as diabetes, aging, stress and alcohol consumption. However, the effects of cell-free extract of ATSCs (ATSC-Ex) containing secretome on wound healing process have not been investigated. In this study, ATSC-Ex was topically applied on the cutaneous wound and healing speed was examined. As a result, wound closure was much faster in the cell-free extract treated wound than control wound at 4, 6, 8 days after application of ATSC-Ex. Dermal fibroblast proliferation, migration and extracellular matrix (ECM) production are critical aspects of wound healing, and the effects of ATSC-Ex on human dermal fibroblast (HDF) was examined. ATSC-Ex augmented HDF proliferation in a dose-dependent manner and migration ability was enhanced by extract treatment. Representative ECM proteins, collagen type I and matrix metalloproteinase-1, are significantly up-regulated by treatment of ATSC-Ex. Our results suggest that the ATSC-Ex have improving effect of wound healing and can be the potential therapeutic candidate for cutaneous wound healing.

Authors+Show Affiliations

Department of Brain and Cognitive Sciences, Tufts University, MA, USA.Department of Neurology, Seoul National University Hospital, Seoul, South Korea.Department of Neurology, Seoul National University Hospital, Seoul, South Korea.Department of Neurology, Seoul National University Hospital, Seoul, South Korea; Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, South Korea. Electronic address: imwooseok@gmail.com.Department of Neurology, Seoul National University Hospital, Seoul, South Korea; Protein Metabolism Medical Research Center, College of Medicine, Seoul National University, Seoul, South Korea. Electronic address: kimmanho@snu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28137582

Citation

Na, You Kyung, et al. "Wound Healing Potential of Adipose Tissue Stem Cell Extract." Biochemical and Biophysical Research Communications, vol. 485, no. 1, 2017, pp. 30-34.
Na YK, Ban JJ, Lee M, et al. Wound healing potential of adipose tissue stem cell extract. Biochem Biophys Res Commun. 2017;485(1):30-34.
Na, Y. K., Ban, J. J., Lee, M., Im, W., & Kim, M. (2017). Wound healing potential of adipose tissue stem cell extract. Biochemical and Biophysical Research Communications, 485(1), 30-34. https://doi.org/10.1016/j.bbrc.2017.01.103
Na YK, et al. Wound Healing Potential of Adipose Tissue Stem Cell Extract. Biochem Biophys Res Commun. 2017 03 25;485(1):30-34. PubMed PMID: 28137582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wound healing potential of adipose tissue stem cell extract. AU - Na,You Kyung, AU - Ban,Jae-Jun, AU - Lee,Mijung, AU - Im,Wooseok, AU - Kim,Manho, Y1 - 2017/01/27/ PY - 2017/01/12/received PY - 2017/01/20/accepted PY - 2017/2/1/pubmed PY - 2017/6/7/medline PY - 2017/2/1/entrez KW - Adipose derived stem cell KW - Collagen KW - Wound healing SP - 30 EP - 34 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 485 IS - 1 N2 - Adipose tissue stem cells (ATSCs) are considered as a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using stem cells, intercellular molecule exchange by stem cell secretory factors showed beneficial effects by reducing tissue damage and augmentation of endogenous repair. Delayed cutaneous wound healing is implicated in many conditions such as diabetes, aging, stress and alcohol consumption. However, the effects of cell-free extract of ATSCs (ATSC-Ex) containing secretome on wound healing process have not been investigated. In this study, ATSC-Ex was topically applied on the cutaneous wound and healing speed was examined. As a result, wound closure was much faster in the cell-free extract treated wound than control wound at 4, 6, 8 days after application of ATSC-Ex. Dermal fibroblast proliferation, migration and extracellular matrix (ECM) production are critical aspects of wound healing, and the effects of ATSC-Ex on human dermal fibroblast (HDF) was examined. ATSC-Ex augmented HDF proliferation in a dose-dependent manner and migration ability was enhanced by extract treatment. Representative ECM proteins, collagen type I and matrix metalloproteinase-1, are significantly up-regulated by treatment of ATSC-Ex. Our results suggest that the ATSC-Ex have improving effect of wound healing and can be the potential therapeutic candidate for cutaneous wound healing. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/28137582/Wound_healing_potential_of_adipose_tissue_stem_cell_extract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(17)30161-4 DB - PRIME DP - Unbound Medicine ER -