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Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice.
Sci Rep. 2017 01 31; 7:41846.SR

Abstract

Leaf senescence is a complex biological process and defense responses play vital role for rice development, their molecular mechanisms, however, remain elusive in rice. We herein reported a rice mutant spotted leaf 32 (spl32) derived from a rice cultivar 9311 by radiation. The spl32 plants displayed early leaf senescence, identified by disintegration of chloroplasts as cellular evidence, dramatically decreased contents of chlorophyll, up-regulation of superoxide dismutase enzyme activity and malondialdehyde, as physiological characteristic, and both up-regulation of senescence-induced STAY GREEN gene and senescence-associated transcription factors, and down-regulation of photosynthesis-associated genes, as molecular indicators. Positional cloning revealed that SPL32 encodes a ferredoxin-dependent glutamate synthase (Fd-GOGAT). Compared to wild type, enzyme activity of GOGAT was significantly decreased, and free amino acid contents, particularly for glutamate and glutamine, were altered in spl32 leaves. Moreover, the mutant was subjected to uncontrolled oxidative stress due to over-produced reactive oxygen species and damaged scavenging pathways, in accordance with decreased photorespiration rate. Besides, the mutant showed higher resistance to Xanthomonas oryzae pv. Oryzae than its wild type, coupled with up-regulation of four pathogenesis-related marker genes. Taken together, our results highlight Fd-GOGAT is associated with the regulation of leaf senescence and defense responses in rice.

Authors+Show Affiliations

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28139777

Citation

Sun, Liting, et al. "Isolation and Characterization of a Spotted Leaf 32 Mutant With Early Leaf Senescence and Enhanced Defense Response in Rice." Scientific Reports, vol. 7, 2017, p. 41846.
Sun L, Wang Y, Liu LL, et al. Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice. Sci Rep. 2017;7:41846.
Sun, L., Wang, Y., Liu, L. L., Wang, C., Gan, T., Zhang, Z., Wang, Y., Wang, D., Niu, M., Long, W., Li, X., Zheng, M., Jiang, L., & Wan, J. (2017). Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice. Scientific Reports, 7, 41846. https://doi.org/10.1038/srep41846
Sun L, et al. Isolation and Characterization of a Spotted Leaf 32 Mutant With Early Leaf Senescence and Enhanced Defense Response in Rice. Sci Rep. 2017 01 31;7:41846. PubMed PMID: 28139777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice. AU - Sun,Liting, AU - Wang,Yihua, AU - Liu,Ling-Long, AU - Wang,Chunming, AU - Gan,Ting, AU - Zhang,Zhengyao, AU - Wang,Yunlong, AU - Wang,Di, AU - Niu,Mei, AU - Long,Wuhua, AU - Li,Xiaohui, AU - Zheng,Ming, AU - Jiang,Ling, AU - Wan,Jianmin, Y1 - 2017/01/31/ PY - 2016/09/23/received PY - 2016/12/29/accepted PY - 2017/2/1/entrez PY - 2017/2/1/pubmed PY - 2018/11/6/medline SP - 41846 EP - 41846 JF - Scientific reports JO - Sci Rep VL - 7 N2 - Leaf senescence is a complex biological process and defense responses play vital role for rice development, their molecular mechanisms, however, remain elusive in rice. We herein reported a rice mutant spotted leaf 32 (spl32) derived from a rice cultivar 9311 by radiation. The spl32 plants displayed early leaf senescence, identified by disintegration of chloroplasts as cellular evidence, dramatically decreased contents of chlorophyll, up-regulation of superoxide dismutase enzyme activity and malondialdehyde, as physiological characteristic, and both up-regulation of senescence-induced STAY GREEN gene and senescence-associated transcription factors, and down-regulation of photosynthesis-associated genes, as molecular indicators. Positional cloning revealed that SPL32 encodes a ferredoxin-dependent glutamate synthase (Fd-GOGAT). Compared to wild type, enzyme activity of GOGAT was significantly decreased, and free amino acid contents, particularly for glutamate and glutamine, were altered in spl32 leaves. Moreover, the mutant was subjected to uncontrolled oxidative stress due to over-produced reactive oxygen species and damaged scavenging pathways, in accordance with decreased photorespiration rate. Besides, the mutant showed higher resistance to Xanthomonas oryzae pv. Oryzae than its wild type, coupled with up-regulation of four pathogenesis-related marker genes. Taken together, our results highlight Fd-GOGAT is associated with the regulation of leaf senescence and defense responses in rice. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28139777/Isolation_and_characterization_of_a_spotted_leaf_32_mutant_with_early_leaf_senescence_and_enhanced_defense_response_in_rice_ L2 - https://doi.org/10.1038/srep41846 DB - PRIME DP - Unbound Medicine ER -