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TGF-β1 protects intestinal integrity and influences Smads and MAPK signal pathways in IPEC-J2 after TNF-α challenge.
Innate Immun. 2017 04; 23(3):276-284.II

Abstract

The aim of this study was to investigate the protective effects of TGF-β1 on intestinal epithelial barrier, as well as canonical Smad and MAPK signal pathways involved in these protection processes by a IPEC-J2 model stimulated with TNF-α. IPEC-J2 monolayers were treated without or with TNF-α in the absence or presence of TGF-β1. The results showed that TGF-β1 pretreatment ameliorated TNF-α-induced intestinal epithelial barrier disturbances as indicated by decrease of transepithelial electrical resistance (TER) and increase of paracellular permeability. TGF-β1 also dramatically alleviated TNF-α-induced alteration of TJ proteins ZO-1 and occludin. Moreover, TGF-β1 pretreatment increased TβRII protein expression in IPEC-J2 monolayers challenged with TNF-α. In addition, a significant increase of Smad4 and Smad7 mRNA was also observed in the TGF-β1 pretreatment after TNF-α challenge compared with the control group. Furthermore, TGF-β1 pretreatment enhanced smad2 protein activation. These results indicated that the canonical Smad signaling pathway was activated by TGF-β1 pretreatment. Finally, TGF-β1 pretreatment decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38) and increased the ratio of ERK (p-ERK/ERK). Anti-TGF-β1 Abs reduced these TGF-β1 effects. These results indicated that TGF-β1 protects intestinal integrity and influences Smad and MAPK signal pathways in IPEC-J2 after TNF-α challenge.

Authors+Show Affiliations

Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.Animal Science College, Zhejiang University; Key Laboratory of Animal Feed and Nutrition of Zhejiang Province; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28142299

Citation

Xiao, Kan, et al. "TGF-β1 Protects Intestinal Integrity and Influences Smads and MAPK Signal Pathways in IPEC-J2 After TNF-α Challenge." Innate Immunity, vol. 23, no. 3, 2017, pp. 276-284.
Xiao K, Cao S, Jiao L, et al. TGF-β1 protects intestinal integrity and influences Smads and MAPK signal pathways in IPEC-J2 after TNF-α challenge. Innate Immun. 2017;23(3):276-284.
Xiao, K., Cao, S., Jiao, L., Song, Z., Lu, J., & Hu, C. (2017). TGF-β1 protects intestinal integrity and influences Smads and MAPK signal pathways in IPEC-J2 after TNF-α challenge. Innate Immunity, 23(3), 276-284. https://doi.org/10.1177/1753425917690815
Xiao K, et al. TGF-β1 Protects Intestinal Integrity and Influences Smads and MAPK Signal Pathways in IPEC-J2 After TNF-α Challenge. Innate Immun. 2017;23(3):276-284. PubMed PMID: 28142299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TGF-β1 protects intestinal integrity and influences Smads and MAPK signal pathways in IPEC-J2 after TNF-α challenge. AU - Xiao,Kan, AU - Cao,Shuting, AU - Jiao,Lefei, AU - Song,Zehe, AU - Lu,Jianjun, AU - Hu,Caihong, Y1 - 2017/01/31/ PY - 2017/2/2/pubmed PY - 2017/6/27/medline PY - 2017/2/2/entrez KW - IPEC-J2 KW - MAPK KW - Smads KW - TGF-β1 KW - intestinal barrier SP - 276 EP - 284 JF - Innate immunity JO - Innate Immun VL - 23 IS - 3 N2 - The aim of this study was to investigate the protective effects of TGF-β1 on intestinal epithelial barrier, as well as canonical Smad and MAPK signal pathways involved in these protection processes by a IPEC-J2 model stimulated with TNF-α. IPEC-J2 monolayers were treated without or with TNF-α in the absence or presence of TGF-β1. The results showed that TGF-β1 pretreatment ameliorated TNF-α-induced intestinal epithelial barrier disturbances as indicated by decrease of transepithelial electrical resistance (TER) and increase of paracellular permeability. TGF-β1 also dramatically alleviated TNF-α-induced alteration of TJ proteins ZO-1 and occludin. Moreover, TGF-β1 pretreatment increased TβRII protein expression in IPEC-J2 monolayers challenged with TNF-α. In addition, a significant increase of Smad4 and Smad7 mRNA was also observed in the TGF-β1 pretreatment after TNF-α challenge compared with the control group. Furthermore, TGF-β1 pretreatment enhanced smad2 protein activation. These results indicated that the canonical Smad signaling pathway was activated by TGF-β1 pretreatment. Finally, TGF-β1 pretreatment decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38) and increased the ratio of ERK (p-ERK/ERK). Anti-TGF-β1 Abs reduced these TGF-β1 effects. These results indicated that TGF-β1 protects intestinal integrity and influences Smad and MAPK signal pathways in IPEC-J2 after TNF-α challenge. SN - 1753-4267 UR - https://www.unboundmedicine.com/medline/citation/28142299/TGF_β1_protects_intestinal_integrity_and_influences_Smads_and_MAPK_signal_pathways_in_IPEC_J2_after_TNF_α_challenge_ L2 - https://journals.sagepub.com/doi/10.1177/1753425917690815?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -