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Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress.
BMC Res Notes. 2017 Feb 01; 10(1):77.BR

Abstract

BACKGROUND

The Curcuma longa L. (CLL) rhizome has long been used to treat patients with hepatic dysfunction. CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed to evaluate the hepatoprotective activity of CLL extract and its active component curcumin in an acute carbon tetrachloride (CCl4)-induced liver stress model.

METHODS

Acute hepatic stress was induced by a single intraperitoneal injection of CCl4 (0.1 ml/kg body weight) in rats. CLL extract was administered once a day for 3 days at three dose levels (100, 200, and 300 mg/kg/day) and curcumin was administered once a day at the 200 mg/kg/day. We performed alanine transaminase (ALT) and aspartate transaminase (AST). activity analysis and also measured total lipid, triglyceride, and cholesterol levels, and lipid peroxidation.

RESULTS

At 100 g CLL, the curcuminoid components curcumin (901.63 ± 5.37 mg/100 g), bis-demethoxycurcumin (108.28 ± 2.89 mg/100 g), and demethoxycurcumin (234.85 ± 1.85 mg/100 g) were quantified through high liquid chromatography analysis. In CCl4-treated rats, serum AST and ALT levels increased 2.1- and 1.2-fold compared with the control. AST but not ALT elevation induced by CCl4 was significantly alleviated in CLL- and curcumin-treated rats. Peroxidation of membrane lipids in the liver was significantly prevented by CLL (100, 200, and 300 mg/kg/day) on tissue lipid peroxidation assay and immunostaining with anti-4HNE antibody. We found that CLL extract and curcumin exhibited significant protection against liver injury by improving hepatic superoxide dismutase (p < 0.05) and glutathione peroxidase activity, and glutathione content in the CCl4-treated group (p < 0.05), leading to a reduced lipid peroxidase level.

CONCLUSION

Our data suggested that CLL extract and curcumin protect the liver from acute CCl4-induced injury in a rodent model by suppressing hepatic oxidative stress. Therefore, CLL extract and curcumin are potential therapeutic antioxidant agents against acute hepatotoxicity.

Authors+Show Affiliations

Department of Pharmacology and New Drug Development Institute, Chonbuk National University Medical School, Jeonju, Chonbuk, 561-180, Republic of Korea.Department of Pharmacology and New Drug Development Institute, Chonbuk National University Medical School, Jeonju, Chonbuk, 561-180, Republic of Korea.Department of Pharmacology and New Drug Development Institute, Chonbuk National University Medical School, Jeonju, Chonbuk, 561-180, Republic of Korea.Department of Pharmacology and New Drug Development Institute, Chonbuk National University Medical School, Jeonju, Chonbuk, 561-180, Republic of Korea.CS1 Center, Ottogi Research Center, Ottogi Corporation, Kyeonggi-do, 14060, Republic of Korea. Chemical Genomics National Research Laboratory, Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-752, Republic of Korea.Department of Pharmacology and New Drug Development Institute, Chonbuk National University Medical School, Jeonju, Chonbuk, 561-180, Republic of Korea. hjchae@jbnu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28143589

Citation

Lee, Geum-Hwa, et al. "Protective Effect of Curcuma Longa L. Extract On CCl4-induced Acute Hepatic Stress." BMC Research Notes, vol. 10, no. 1, 2017, p. 77.
Lee GH, Lee HY, Choi MK, et al. Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Res Notes. 2017;10(1):77.
Lee, G. H., Lee, H. Y., Choi, M. K., Chung, H. W., Kim, S. W., & Chae, H. J. (2017). Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Research Notes, 10(1), 77. https://doi.org/10.1186/s13104-017-2409-z
Lee GH, et al. Protective Effect of Curcuma Longa L. Extract On CCl4-induced Acute Hepatic Stress. BMC Res Notes. 2017 Feb 1;10(1):77. PubMed PMID: 28143589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. AU - Lee,Geum-Hwa, AU - Lee,Hwa-Young, AU - Choi,Min-Kyung, AU - Chung,Han-Wool, AU - Kim,Seung-Wook, AU - Chae,Han-Jung, Y1 - 2017/02/01/ PY - 2016/03/05/received PY - 2017/01/25/accepted PY - 2017/2/2/entrez PY - 2017/2/2/pubmed PY - 2017/2/17/medline KW - CLL extract KW - Curcumin KW - GSH KW - Hepatotoxicity KW - Lipid peroxidation SP - 77 EP - 77 JF - BMC research notes JO - BMC Res Notes VL - 10 IS - 1 N2 - BACKGROUND: The Curcuma longa L. (CLL) rhizome has long been used to treat patients with hepatic dysfunction. CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed to evaluate the hepatoprotective activity of CLL extract and its active component curcumin in an acute carbon tetrachloride (CCl4)-induced liver stress model. METHODS: Acute hepatic stress was induced by a single intraperitoneal injection of CCl4 (0.1 ml/kg body weight) in rats. CLL extract was administered once a day for 3 days at three dose levels (100, 200, and 300 mg/kg/day) and curcumin was administered once a day at the 200 mg/kg/day. We performed alanine transaminase (ALT) and aspartate transaminase (AST). activity analysis and also measured total lipid, triglyceride, and cholesterol levels, and lipid peroxidation. RESULTS: At 100 g CLL, the curcuminoid components curcumin (901.63 ± 5.37 mg/100 g), bis-demethoxycurcumin (108.28 ± 2.89 mg/100 g), and demethoxycurcumin (234.85 ± 1.85 mg/100 g) were quantified through high liquid chromatography analysis. In CCl4-treated rats, serum AST and ALT levels increased 2.1- and 1.2-fold compared with the control. AST but not ALT elevation induced by CCl4 was significantly alleviated in CLL- and curcumin-treated rats. Peroxidation of membrane lipids in the liver was significantly prevented by CLL (100, 200, and 300 mg/kg/day) on tissue lipid peroxidation assay and immunostaining with anti-4HNE antibody. We found that CLL extract and curcumin exhibited significant protection against liver injury by improving hepatic superoxide dismutase (p < 0.05) and glutathione peroxidase activity, and glutathione content in the CCl4-treated group (p < 0.05), leading to a reduced lipid peroxidase level. CONCLUSION: Our data suggested that CLL extract and curcumin protect the liver from acute CCl4-induced injury in a rodent model by suppressing hepatic oxidative stress. Therefore, CLL extract and curcumin are potential therapeutic antioxidant agents against acute hepatotoxicity. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/28143589/Protective_effect_of_Curcuma_longa_L__extract_on_CCl4_induced_acute_hepatic_stress_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2409-z DB - PRIME DP - Unbound Medicine ER -