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Neisseria gonorrhoeae DNA bacterial load in men with symptomatic and asymptomatic gonococcal urethritis.
Sex Transm Infect. 2017 11; 93(7):478-481.ST

Abstract

OBJECTIVE

Previous studies have quantified bacterial loads of Neisseria gonorrhoeae in the pharynx and rectum of men but not the urethra. We quantified the bacterial load of N. gonorrhoeae in men with symptomatic and asymptomatic urethral gonorrhoea infections.

METHODS

Consecutive men diagnosed with urethral gonorrhoea by Aptima Combo 2 testing of urine at the Melbourne Sexual Health Centre between March and July 2016 were eligible for the study: symptomatic men with purulent urethral discharge and asymptomatic men with no urethral symptoms. The gonococcal bacterial load in both groups was measured by urethral swab using a standardised collection method and real-time quantitative PCR targeting the opa gene.

RESULTS

Twenty men were recruited into the study: 16 had purulent urethral discharge and 4 had asymptomatic urethral gonorrhoea. The median gonococcal bacterial load was significantly higher among symptomatic men (3.7×106 copies per swab, IQR 2.5×106-4.7×106) compared with asymptomatic men (2.0×105 copies per swab, IQR 2.7×104-4.5×105) (p=0.002).

CONCLUSIONS

Gonococcal loads in men with urethral discharge were higher than loads seen with asymptomatic urethral gonorrhoea and loads seen in asymptomatic pharyngeal and rectal gonorrhoea infections in previous studies.

Authors+Show Affiliations

Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia. Regional HPV Lab Net Reference laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia. Regional HPV Lab Net Reference laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia. Regional HPV Lab Net Reference laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.Melbourne Sexual Health Centre, Carlton, Victoria, Australia. Central Clinical School, Monash University, Clayton, Victoria, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28148678

Citation

Priest, David, et al. "Neisseria Gonorrhoeae DNA Bacterial Load in Men With Symptomatic and Asymptomatic Gonococcal Urethritis." Sexually Transmitted Infections, vol. 93, no. 7, 2017, pp. 478-481.
Priest D, Ong JJ, Chow EPF, et al. Neisseria gonorrhoeae DNA bacterial load in men with symptomatic and asymptomatic gonococcal urethritis. Sex Transm Infect. 2017;93(7):478-481.
Priest, D., Ong, J. J., Chow, E. P. F., Tabrizi, S., Phillips, S., Bissessor, M., Fairley, C. K., Bradshaw, C. S., Read, T. R. H., Garland, S., & Chen, M. (2017). Neisseria gonorrhoeae DNA bacterial load in men with symptomatic and asymptomatic gonococcal urethritis. Sexually Transmitted Infections, 93(7), 478-481. https://doi.org/10.1136/sextrans-2016-052950
Priest D, et al. Neisseria Gonorrhoeae DNA Bacterial Load in Men With Symptomatic and Asymptomatic Gonococcal Urethritis. Sex Transm Infect. 2017;93(7):478-481. PubMed PMID: 28148678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neisseria gonorrhoeae DNA bacterial load in men with symptomatic and asymptomatic gonococcal urethritis. AU - Priest,David, AU - Ong,Jason J, AU - Chow,Eric P F, AU - Tabrizi,Sepehr, AU - Phillips,Sam, AU - Bissessor,Melanie, AU - Fairley,Christopher K, AU - Bradshaw,Catriona S, AU - Read,Tim R H, AU - Garland,Suzanne, AU - Chen,Marcus, Y1 - 2017/02/01/ PY - 2016/10/23/received PY - 2016/12/20/revised PY - 2017/01/11/accepted PY - 2017/2/6/pubmed PY - 2018/1/16/medline PY - 2017/2/3/entrez KW - BACTERIAL INFECTION KW - GONORRHOEA KW - MODES OF TRANSMISSION KW - NEISSERIA GONORRHOEA KW - URETHRITIS SP - 478 EP - 481 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 93 IS - 7 N2 - OBJECTIVE: Previous studies have quantified bacterial loads of Neisseria gonorrhoeae in the pharynx and rectum of men but not the urethra. We quantified the bacterial load of N. gonorrhoeae in men with symptomatic and asymptomatic urethral gonorrhoea infections. METHODS: Consecutive men diagnosed with urethral gonorrhoea by Aptima Combo 2 testing of urine at the Melbourne Sexual Health Centre between March and July 2016 were eligible for the study: symptomatic men with purulent urethral discharge and asymptomatic men with no urethral symptoms. The gonococcal bacterial load in both groups was measured by urethral swab using a standardised collection method and real-time quantitative PCR targeting the opa gene. RESULTS: Twenty men were recruited into the study: 16 had purulent urethral discharge and 4 had asymptomatic urethral gonorrhoea. The median gonococcal bacterial load was significantly higher among symptomatic men (3.7×106 copies per swab, IQR 2.5×106-4.7×106) compared with asymptomatic men (2.0×105 copies per swab, IQR 2.7×104-4.5×105) (p=0.002). CONCLUSIONS: Gonococcal loads in men with urethral discharge were higher than loads seen with asymptomatic urethral gonorrhoea and loads seen in asymptomatic pharyngeal and rectal gonorrhoea infections in previous studies. SN - 1472-3263 UR - https://www.unboundmedicine.com/medline/citation/28148678/Neisseria_gonorrhoeae_DNA_bacterial_load_in_men_with_symptomatic_and_asymptomatic_gonococcal_urethritis_ L2 - http://sti.bmj.com/cgi/pmidlookup?view=long&pmid=28148678 DB - PRIME DP - Unbound Medicine ER -