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Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.
Plant Mol Biol. 2017 Apr; 93(6):607-621.PM

Abstract

Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control.

Authors+Show Affiliations

Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468, C1440FFX, Buenos Aires, Argentina.Facultad de Bioquímica y Farmacia-Instituto de Biología Celular y Molecular de Rosario, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina.Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150, T4101XAC, Las Talitas, Tucumán, Argentina. paulafilippone@eeaoc.org.ar.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28155188

Citation

Sendín, Lorena Noelia, et al. "Inducible Expression of Bs2 R Gene From Capsicum Chacoense in Sweet Orange (Citrus Sinensis L. Osbeck) Confers Enhanced Resistance to Citrus Canker Disease." Plant Molecular Biology, vol. 93, no. 6, 2017, pp. 607-621.
Sendín LN, Orce IG, Gómez RL, et al. Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease. Plant Mol Biol. 2017;93(6):607-621.
Sendín, L. N., Orce, I. G., Gómez, R. L., Enrique, R., Grellet Bournonville, C. F., Noguera, A. S., Vojnov, A. A., Marano, M. R., Castagnaro, A. P., & Filippone, M. P. (2017). Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease. Plant Molecular Biology, 93(6), 607-621. https://doi.org/10.1007/s11103-017-0586-8
Sendín LN, et al. Inducible Expression of Bs2 R Gene From Capsicum Chacoense in Sweet Orange (Citrus Sinensis L. Osbeck) Confers Enhanced Resistance to Citrus Canker Disease. Plant Mol Biol. 2017;93(6):607-621. PubMed PMID: 28155188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease. AU - Sendín,Lorena Noelia, AU - Orce,Ingrid Georgina, AU - Gómez,Rocío Liliana, AU - Enrique,Ramón, AU - Grellet Bournonville,Carlos Froilán, AU - Noguera,Aldo Sergio, AU - Vojnov,Adrián Alberto, AU - Marano,María Rosa, AU - Castagnaro,Atilio Pedro, AU - Filippone,María Paula, Y1 - 2017/02/02/ PY - 2016/07/19/received PY - 2017/01/13/accepted PY - 2017/2/6/pubmed PY - 2017/4/4/medline PY - 2017/2/4/entrez KW - Agrobacterium-mediated transformation KW - AvrBs2 gene KW - Citrus breeding KW - Glutathione S-transferase promoter KW - Resistance genes KW - Xanthomonas citri subsp. citri SP - 607 EP - 621 JF - Plant molecular biology JO - Plant Mol Biol VL - 93 IS - 6 N2 - Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/28155188/Inducible_expression_of_Bs2_R_gene_from_Capsicum_chacoense_in_sweet_orange__Citrus_sinensis_L__Osbeck__confers_enhanced_resistance_to_citrus_canker_disease_ L2 - https://doi.org/10.1007/s11103-017-0586-8 DB - PRIME DP - Unbound Medicine ER -